The Effect of High-Intensity Interval Training (HIIT) on the Contents of Dynamin-Like Protein 1 (DRP1) and Optic Atrophy 1 (OPA1) in the Left Ventricle of Aged Male Rats

Document Type : Original Article (s)

Authors

1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

2 Department of Physical Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran

Abstract

Background: Aging is accompanied by a gradual weakening of heart function, which can lead to an increase in the risk of cardiovascular diseases. HIIT, as a potent exercise intervention, seems to benefit heart function and mitochondrial health in elderly groups.
Methods: The present research is of an experimental type, conducted with 12 20-month-old Wistar male rats with an average weight of 400 ± 30 grams. The inclusion criteria were male rats aged 20 months and older, which were randomly divided into two HIIT and control groups (6 rats in each group). During the research, the control group had no activity, and the training group ran on the treadmill for eight weeks and five sessions weekly. The HIIT program was performed with high-intensity bouts of 85-95% of maximum speed (m/min) and active rest periods with an intensity of 40-55% of maximum speed (m/min) for each session per week. After 48 hours following the last training session, the rats were anesthetized, and the variables were measured using the western blot method. The data were analyzed through Shapiro-Wilk and independent t-test, and the significance level was considered P ≥ 0.05.
Findings: The results showed that eight weeks of HIIT caused a significant increase in DRP1 in the hearts of old rats, while it did not significantly change the content of OPA1 protein.
Conclusion: HIIT protocol seems to improve mitochondrial quality control in the heart of aged rats.

Highlights

Neda Aghaei Bahmanbeglou: Google Scholar, PubMed

Hamed Alizadeh Pahlavani: Google Scholar, PubMed

Habib Asgharpour: Google Scholar

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 42, Issue 776
4th Week, September
September and October 2024
Pages 615-623

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History

  • Receive Date: 22 July 2024
  • Accept Date: 03 September 2024

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