Isolation and Propagation of Colon Cancer Stem Cells In vit

Document Type : Original Article (s)

Authors

1 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan

2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Associate Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

6 Associate Professor, Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran

7 Assistant Professor, Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

8 Associate Professor, Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Recent investigations in colon cancer biology suggest that cancer growth is driven by colon cancer stem cells (CSCs). CSCs are responsible for tumor initiation, maintenance, spreading and relapse. The aim of this study was to isolate and propagate tumorigenic colon CSCs in vitro.Methods: Tumor samples from colon cancers were subjected to mechanical and enzymatic disassociation. After passing of tumor cell suspensions through the cell strainers, tumor cells were counted and their viability was determined. Then tumor cells were plated in the special stem cells medium (contains serum-free DMEM/F12 supplemented by growth factors). Tumor cells growth was evaluated by inverted microscope.Findings: After about 3 weeks of plating in specific medium, colon CSCs were appeared as cell spheres whereas differentiated tumor cells and nonmalaginent cells were unable to grow. Colon cancer spheres were able to propagated and passaged in the specific stem cells medium.Conclusion: Isolation and propagation of tumorigenic colon CSCs in vitro would help to devise novel diagnostic and therapeutic methods. Isolated colon CSCs can be applied for the studying of cell signaling and assessment of the effect of anti-cancer drugs.

Keywords

References

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Journal of Isfahan Medical School
Volume 29, Issue 154 - Serial Number 154
July and August 2011
Pages 1356-1364

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History

  • Receive Date: 05 October 2011
  • Revise Date: 27 May 2024
  • Accept Date: 06 April 2022

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