The Effect of Bystander Due to Combined Treatment of Ultrasound Waves and Cisplatin on Melanoma A375 Cell Line

Document Type : Original Article (s)

Authors

1 Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The use of ultrasound waves in combination with chemotherapy drugs increases the effectiveness of the drug in low doses. Moreover, in the treatment process, cells that have not been directly treated undergo a variety of changes such as death; this phenomenon is known as the bystander effect. This study aimed to investigate the effect of combined treatment of ultrasound waves and cisplatin on the bystander cells in melanoma cells.Methods: Melanoma A375 cells were first cultured in the laboratory. After culturing the cells, two target and bystander cell groups were considered for the experiments. After determining the optimal concentration of cisplatin for melanoma A375 cells by MTT test, the target group cells were treated with the optimal concentration of cisplatin and ultrasound with intensities of 0.5, 1, and 2 w/cm2. After 24 hours, the culture medium of the target cells was collected and added to the bystander cells using the medium transfer technique. At 24 hours, MTT assay was used to measure cell viability.Findings: The survival percentage of melanoma cells in the bystander group, which received the culture medium of the treated cells by combination of cisplatin and ultrasound waves with an intensity of 2 W/cm2, had a significant decrease compared to the control group.Conclusion: In combination therapies with ultrasound waves and chemotherapy drugs, the role of the bystander effect on the efficiency of treatment and cell survival should also be considered.

Keywords

References

  1. Pawlik TM, Sondak VK. Malignant melanoma: Current state of primary and adjuvant treatment. Crit Rev Oncol Hematol 2003; 45(3): 245-64.
  2. Shanei A, Baradaran M, Shanei MM. The effect of ultrasound waves on melanoma cells in presence of gold nanoparticles. J Isfahan Med Sch 2017; 34(412): 1550-5. [In Persian].
  3. Power ME, Olson ME, Domingue PA, Costerton JW. A rat model of Staphylococcus aureus chronic osteomyelitis that provides a suitable system for studying the human infection. J Med Microbiol 1990; 33(3): 189-98.
  4. Yahyapour R, Motevaseli E, Rezaeyan A, Abdollahi H, Farhood B, Cheki M, et al. Mechanisms of radiation bystander and non-targeted effects: Implications to radiation carcinogenesis and radiotherapy. Curr Radiopharm 2018; 11(1): 34-45.
  5. Marin A, Martin M, Linan O, Alvarenga F, Lopez M, Fernandez L, et al. Bystander effects and radiotherapy. Rep Pract Oncol Radiother 2015; 20(1): 12-21.
  6. Zefferino R, Piccoli C, Gioia SD, Capitanio N, Conese M. Gap junction intercellular communication in the carcinogenesis hallmarks: Is this a phenomenon or epiphenomenon? Cells 2019; 8(8): 896.
  7. Bazak J, Fahey JM, Wawak K, Korytowski W, Girotti AW. Bystander effects of nitric oxide in anti-tumor photodynamic therapy. Cancer Cell Microenviron 2017; 4(1): e1511.
  8. Rezaei M, Kamran Samani R, Kazemi M, Shanei A, Hejazi S. Induction of a bystander effect after therapeutic ultrasound exposure in human melanoma: In-vitro assay. Int J Radiat Res 2021; 19 (1): 183-9.
  9. Sabel MS, Sondak VK. Pros and cons of adjuvant interferon in the treatment of melanoma. Oncologist 2003; 8(5): 451-8.
  10. Sazgarnia A, Shanei A, Eshghi H, Hassanzadeh-Khayyat M, Esmaily H, Shanei MM. Detection of sonoluminescence signals in a gel phantom in the presence of Protoporphyrin IX conjugated to gold nanoparticles. Ultrasonics 2013; 53(1): 29-35.

Files

History

  • Receive Date: 04 July 2021
  • Revise Date: 23 May 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics