Effect of Adenosine Receptor Agonist and Ascorbic Acid on Ultrastructure of Hippocampal CA1 Neurons after Ischemia-Reperfusion Injury

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Anatomy, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

2 Associate Professor, Cellular and Molecular Research Center AND Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

3 Assistant Professor, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

4 Associate Professor, Department of Anatomy, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Abstract

Background: In brain ischemia, blood and oxygen supply decrease and after reperfusion, free radicals and reactive oxygen species (ROS) cause severe damage. As hippocampal injury after ischemia-reperfusion causes some complications, in this study we analyzed the effect of adenosine receptor agonist (N6-cyclopentyladenosine or CPA) and ascorbic acid on ultrastructure of hippocampal CA1 neurons after ischemia-reperfusion.Methods: 35 male rats in 5 groups were used. Ischemia-reperfusion performed by occlusion of common carotids for 15 minutes. CPA and ascorbic acids were intraperitoneally injected for 7 days after ischemia, and 2 weeks before and for 7 days after ischemia, respectively. After 20 days, brain samples were isolated, prepared, and assayed using transmission electron microscopy (TEM).Findings: Ultrastructure assay of hippocampal CA1 neurons after ischemia-reperfusion with transmission electron microscopy showed recovery of intracellular organelles particularly mitochondria of treated groups. In combination therapy, these improvements were better.Conclusion: Intraperitoneal injection of CPA and ascorbic acid after ischemia-reperfusion can reduce neural damage in CA1 region of hippocampus.

Keywords

References

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Journal of Isfahan Medical School
Volume 35, Issue 439 - Serial Number 439
May and June 2017
Pages 862-869

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History

  • Receive Date: 16 May 2017
  • Revise Date:
  • Accept Date: 06 April 2022

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