Association Analysis of T-Cell Immunoglobulin and Mucin 1 Gene 5383-5397 Insertion/Deletion Polymorphism with Asthma and Total Serum Immunoglobulin E Levels in Patients with Asthma and Controls

Document Type : Original Article (s)

Authors

1 Professor, Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Assistant Professor, Cellular and Molecular Immunology Research Center AND Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Subspecialist in Asthma, Allergy and Clinical Immunology, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences Isfahan, Iran

6 Professor, Department of Genetics and Molecular Biology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

7 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

8 Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Background: A member of a T-cell immunoglobulin (Ig) domain and mucin domain (TIM) gene family, TIM-1, in 5q31-33 region is shown to be correlated with development of T helper-2 (TH2) cells and allergic diseases. Polymorphism insertion/deletion (ins/del) of 5383-5397 may affect the length of TIM-1 mucin domain. The aim of this study was to investigate the association of polymorphism insertion/deletion (ins/del) 5383-5397 and susceptibility to asthma in Chaharmahal va Bakhtiari Province, Iran.Methods: Polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) were used for investigating the presence of (ins/del) 5383-5397 polymorphism in 300 patients with asthma and healthy controls. Total IgE was measured via the enzyme-linked immunosorbent assay (ELISA) technique in their serum samples as well.Findings: Significant associations were found between the insertion/insertion genotype in 5383-5397 insertion/deletion polymorphism with both asthma and levels of total serum IgE in patients with asthma (P < 0.05). Furthermore, we found that the frequency of the deletion allele is significantly lower in patients with asthma.Conclusion: Based on these findings, we identified a significant association between 5383-5397 ins/del polymorphism with asthma and with total serum IgE levels in Chaharmahal va Bakhtiari Province population.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 300 - Serial Number 300
July and August 2014
Pages 1423-1432

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History

  • Receive Date: 08 June 2014
  • Revise Date:
  • Accept Date: 06 April 2022

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