اثر فعالیت شنا بر محتوای پروتئین‌های AIM2، ASC و Caspase1 بافت هیپوکمپ موش‌های صحرایی نر نژاد ویستار مبتلا به مالتیپل اسکلروزیس

نوع مقاله : Original Article(s)

نویسندگان

1 کارشناسی ارشد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

10.48305/jims.v43.i836.1354

چکیده

مقاله پژوهشی




مقدمه: مالتیپل اسکلروزیس، یک بیماری خودایمنی است که به سیستم عصبی مرکزی آسیب می‌زند و باعث تخریب میلین می‌شود. شنا، به عنوان یک فعالیت ترکیبی هوازی و مقاومتی، می‌تواند تغییرات بافت هیپوکامپ ناشی از (Multiple Sclerosis) MS را تنظیم کند.
روش‌ها: در پژوهش تجربی حاضر از 32 سر موش صحرایی با مانگین سنی12 هفته استفاده و به‌طور تصادفی به 4 گروه 8‌تایی تقسیم شدند: کنترل، کنترل بیمار، تمرین، تمرین بیمار. مدل بیماری MS با استفاده از غذای کوپریزون 0/5 درصد ایجاد شد. پس از تأیید القای MS از طریق آزمون روتارود، پروتکل شنا به مدت 6 هفته انجام گرفت. بدین صورت که در هفته‌ی اول 10 دقیقه فعالیت شنا را بدون اعمال بار انجام دادند و به منظور اعمال اضافه بار مدت زمان شنا در هر هفته پنج دقیقه اضافه شد. میزان پروتئین‌ها در بافت هیپوکمپ با استفاده از روش وسترن بلات اندازه‌گیری شد. داده‌ها به وسیله‌ی آزمون ANOVA یک راهه و Tukey بررسی شد.
یافته‌ها: نتایج نشان داد محتوای پروتئین‌های AIM2، ASC و Caspase-1 در بافت هیپوکمپ در گروه کنترل بیمار نسبت به گروه کنترل سالم افزایش معنی‌داری داشت (0/05 > P). همچنین نتایج مقادیر پروتئین‌ها در گروه تمرین بیمار نسبت به گروه کنترل بیمار کاهش معنی‌داری داشت (0/05 > P).
نتیجه‌گیری: بر اساس نتایج مطالعه‌ی حاضر احتمالاً می‌توان گفت فعالیت شنا به‌طور معنی‌داری سطح پروتئین AIM2 را در بافت هیپوکمپ موش‌های صحرایی نر نژاد ویستار مبتلا به مالتیپل اسکلروزیس افزایش می‌دهد، که نشان‌دهنده‌ی نقش این پروتئین در پاسخ‌های ایمنی و التهابی مرتبط با بیماری است.

تازه های تحقیق

محمد رمی: Google Scholar, PubMed

روح اله رنجبر: Google Scholar, PubMed

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Swimming Activity on the Content of AIM2, ASC and Caspase1 Proteins in the Hippocampal Tissue of Male Wistar Rats with Multiple Sclerosis

نویسندگان [English]

  • Mona Sadat Kimiaei 1
  • Mohammad Rami 2
  • Rouhollah Ranjbar 3
1 MSc, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Associate Professor, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Background: Multiple sclerosis is an autoimmune disease that damages the central nervous system and causes myelin destruction. Swimming, as a combined aerobic and resistance activity, can modulate the changes in hippocampal tissue caused by MS.
Methods: In the present experimental study, 32 12-week-old male rats with MS were used and randomly divided into 4 groups of 8: control, patient control, training, and patient training. The MS disease model was created using 0.5% cuprizone diet. After confirming MS induction through the rotarod test, the swimming protocol was performed for 6 weeks. In the first week, 10 minutes of swimming activity was performed without applying load, and in order to apply overload, the swimming time was increased by five minutes each week. The amount of proteins in the hippocampal tissue was measured using the Masteron blot method. The data were analyzed by one-way and Tukey test. ANOVA.
Findings: The results showed that the content of AIM2, ASC and Caspase-1 proteins in the hippocampal tissue in the patient control group was significantly increased compared to the healthy control group (P < 0.05). Also, the results of the protein levels in the patient exercise group were significantly decreased compared to the patient control group (P < 0.05).
Conclusion: Based on the results of the present study, it can be said that swimming activity significantly increases the level of AIM2 protein in the hippocampal tissue of male Wistar rats with multiple sclerosis, which indicates the role of this protein in the immune and inflammatory responses associated with the disease.

کلیدواژه‌ها [English]

  • AIM2
  • ASC
  • Caspase-1
  • Hippocampus
  • Multiple Sclerosis

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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 836
هفته 4، آذر
مهر و آبان 1404
صفحه 1354-1363

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سابقه مقاله

  • تاریخ دریافت: 02 اردیبهشت 1404
  • تاریخ پذیرش: 16 آذر 1404

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