Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen with high antibiotic resistance and biofilm production capability in the patients with burn wound. The present study aimed to determine the antibiotic resistance pattern and frequency of the multidrug-resistant (MDR) strains, and evaluate biofilm formation potential in the burn wound infection isolates.Methods: Biochemical and molecular tests were used for identification of the 117 strains collected from Isfahan City, Iran, during one-year period. Antibacterial susceptibility test was performed using disk diffusion and interpreted in accordance with the standards of Clinical and Laboratory Standards Institute (CLSI). Biofilm formation was evaluated using microtiter plate assay.Findings: Of 117 clinical strains of A. baumannii, 114 (97.49%) were MDR. Strains showed maximum resistant to ceftazidime (99.14%) followed by ciprofloxacin (94.01%). 81.19% of isolates were able to form biofilm, 1.7% of strains were strong-biofilm producers, and 18.80% of them were notable biofilm producers. In this assessment, 42.73% and 36.75% were considered as moderate and weak biofilm-forming isolates, respectively.Conclusion: The high prevalence of A. baumannii in patients with burn wound, and the significant relationship between biofilm formation and resistance among the burn isolates indicate that antibiotic resistance is increasing rapidly, and soon many usable antibiotics will become ineffective medicines for treating patients. This needs an emergency actions and alternative ways to control these problems.

Keywords

References

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Journal of Isfahan Medical School
Volume 37, Issue 552 - Serial Number 552
September and October 2020
Pages 1280-1285

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History

  • Receive Date: 29 December 2019
  • Revise Date: 01 June 2024
  • Accept Date: 06 April 2022

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