تأثیر تمرینات هوازی همراه با مصرف ترکیب گالیک اسید و کامپفرول بر نوروژنز ناشی از عوارض جانبی مصرف داروی پاکلیتاکسل در موش‌های مبتلا به سرطان پستان

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

2 دانشیار، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

چکیده

مقاله پژوهشی




مقدمه: هدف پژوهش حاضر، بررسی تمرینات هوازی همراه با مصرف گالیک اسید و کامپفرول بر نوروژنر ناشی از عوارض جانبی مصرف داروی پاکلیتاکسل در موش‌های مبتلا به سرطان پستان بود.
روش‌ها: 36 سر موش ماده BALB/c به صورت تصادفی به 6 گروه تقسیم شدند که شامل گروه شاهد منفی، گروه سرطان پستان (القا با تزریق رده‌ی سلولی 4T1)، سرطانی- شیمی‌درمانی شده (۱۰ میلی‌گرم بر کیلوگرم وزن بدن موش، تزریق پاکلیتاکسل)، سرطانی شیمی‌درمانی شده+مکمل (گالیک اسید و کامپفرول)، سرطانی شیمی‌درمانی شده+تمرین هوازی و سرطانی شیمی‌درمانی شده+مکمل+تمرین هوازی به مدت هشت هفته انجام شد. بیان ژن‌ها به روش Real Time-PCR سنجیده شد. ژن‌ها به کمک نرم‌افزارهای بیوانفورماتیک انتخاب شدند.
یافته‌ها: میزان بیان JAG1 در گروه شیمی‌درمانی کاهش یافته است. بیان JAG1 در اثر مکمل‌های گالیک اسید و کامپفرول و تمرینات هوازی بطور معنی‌داری نسبت به موش‌های القا شده به سرطان سینه و شیمی‌درمانی شده کاهش یافته است. مصرف همزمان مکمل‌های گالیک اسید و کامپفرول همراه با انجام تمرینات هوازی، سبب کاهش معنی‌دار بیان ژن JAG1 نسبت به سایر گروه‌ها شده است. میزان بیان ژن‌های BDNF و NGF در گروه سرطانی شیمی‌درمانی شده+مکمل، سرطانی شیمی‌درمانی شده+تمرین هوازی افزایش یافته است. بیان BDNF و NGF در گروه سرطانی شیمی‌درمانی شده+مکمل+تمرین هوازی بطور معنی‌دار نسبت به سایر گروه‌ها افزایش یافت.
نتیجه‌گیری: تمرینات هوازی+مکمل، سبب کاهش عوارض جانبی پاکلیتاکسل و افزایش میزان نوروژنز گردید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Aerobic Exercise Combined with the Combination of Gallic Acid and Kaempferol on Neurogenesis Caused by the Side Effects of Paclitaxel in Mice with Breast Cancer

نویسندگان [English]

  • Zeytoon Malekpoor 1
  • Farzaneh Taghian 2
  • khosro Jalali Dehkordi 2
1 PhD Student, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 Associate Professor, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
چکیده [English]

Background: The research aims to determine the effect of aerobic exercise, Gallic acid, and Kaempferol on neurogenesis caused by the side effects of the paclitaxel drug in mice with breast cancer.
Methods: 36 female BALB/c mice were randomly divided in to six groups, including the negative control group, breast cancer group (induced with 4T1 cell line injection), cancer-chemotherapy group (10 mg/kg injected paclitaxel), cancer mice treated with chemotherapy+supplement (Gallic acid and Kaempferol), cancer mice treated with chemotherapy+aerobic exercise, cancer mice treated with chemotherapy+supplement+aerobic exercise for eight weeks. The gene expressions were measured by the Real Time-PCR method. Genes were selected using bioinformatics software.
Findings: The expression level of JAG1 was decreased in the chemotherapy group. Moreover, the expression level of JAG1 was significantly reduced by Gallic acid, Kaempferol supplements, and aerobic exercise compared with the breast cancer and cancer-chemotherapy groups. In addition, Gallic acid and Kaempferol supplements, along with aerobic exercise, significantly declined JAG1 gene expression compared with other groups. The expression levels of the BDNF and NGF genes were increased in the cancer group treated with chemotherapy+supplement and chemotherapy+aerobic exercise group. The expression genes of BDNF and NGF in the cancer group treated with chemotherapy+supplements+aerobic exercise significantly increased compared to other groups.
Conclusion: Aerobic exercises+supplements declined the side effects of paclitaxel and improved the neurogenesis.

کلیدواژه‌ها [English]

  • Chemotherapy-Induced cognitive impairments
  • Aerobic exercise
  • Breast cancer
  • Paclitaxel
  • Gallic acid
  • Kaempferol
  • Neurogenesis

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 722
هفته 2 مرداد
مرداد و شهریور 1402
صفحه 427-437

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  • تاریخ دریافت: 06 فروردین 1402
  • تاریخ پذیرش: 15 تیر 1402

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