Effect of RGD Immobilization on Biocompatibility of Oxidized Cellulose Scaffold in Bone Tissue Engineering

Document Type : Original Article (s)

Authors

1 Instructor, Department of Radiology, School of Paramedical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran

2 Assistant Professor, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

3 Associate Professor, Department of Organic Chemistry, School of Chemistry, Iran University of Science and Technology, Tehran, Iran

4 PhD Student, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

5 Instructor, Department of Operating Room, School of Nursing and Midwifery, Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

Background: Human tissue failures caused by different damages or injuries are the most serious and costly problems in health care and have direct effect on life quality. Tissue Engineering, as a scaffold-based strategy, provides promising research field and may offer innovative viewpoints to treat diseases. Scientists in various fields have tried to functionalize polymers to achieve special surface cell interactions.Methods: Cellulose powder was oxidized with NO2 gas and the porous scaffold was fabricated via dry pressing. RGD peptide was immobilized on the surface of scaffold via grafting to make a hybrid scaffold. The hybrid scaffold was characterized by FTIR (Fourier transform infrared spectroscopy) and SEM (Scanning electron microscope) and its biocompatibility was examined through MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.Findings: FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed via SEM. The results of MTT showed significant increase of viable cells on hybrid scaffold.Conclusion: Porous structure and high biocompatibility were benefits of prepared hybrid scaffold. Cellulose oxidation can present suitable condition for RGD immobilization caused to enhance biocompatibility. In addition, existing pores in good size conditioned hybrid scaffold to engineer bone tissue.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 352 - Serial Number 352
July and August 2015
Pages 1597-1606

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History

  • Receive Date: 18 April 2015
  • Revise Date: 26 May 2024
  • Accept Date: 06 April 2022

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