تعدیل مسیر Nrf1/Sod1/Hmox1 توسط مصرف سینئول و تمرین ورزشی در محیط غنی‌شده جهت حفاظت کبد در برابر سمیت آکریل‌آمید

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران

2 استاد، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران

3 دانشیار، گروه فیزولوژی ورزشی، دانشکده‌ی علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران

10.48305/jims.v43.i833.1228

چکیده

مقاله پژوهشی




مقدمه: استرس اکسیداتیو، ناشی از چاقی و مواجهه با آکریل‌آمید، توانایی دفاع‌های آنتی‌اکسیدانی را کاهش می‌دهد. این پژوهش، اثرات مداخله‌ی ترکیبی فعالیت ‌بدنی در محیط غنی‌شده و مکمل سینئول بر تعدیل بیان ژن‌های مؤثر بر مسیر Nrf2/SOD1/HMOX1 در موش‌های القاء‌ شده‌ی چاق را مورد ارزیابی قرار داد.
روش‌ها: پژوهش حاضر از نوع تجربی و بنیادی می‌باشد. ۵۴ سر موش ماده C57BL/6 6-7 هفته‌ای به‌صورت تصادفی در ۹ گروه تقسیم شدند: کنترل، چاق با رژیم پرچرب حاوی آکریل‌آمید، چاق با رژیم پرچرب بدون آکریل‌آمید، چاق با رژیم آکریل‌آمید و تمرین در محیط غنی‌شده (8 هفته، 5 روز/هفته، 60-90 دقیقه روزانه، شدت کم- متوسط)، چاق با رژیم آکریل‌آمید و تیمار سینئول (غلظت 10 میکرومولار به صورت گاواژ)، چاق با محیط غنی‌شده، چاق با سینئول، چاق با رژیم آکریل‌آمید، محیط غنی‌شده و سینئول، چاق با محیط غنی‌شده و سینئول. بیان ژن‌ها Nrf2/SOD1/HMOX1 در بافت کبد با روش Real-Time-qPCR بررسی شد. داده‌ها با آزمون‌های آنالیز واریانس یک و سه راهه، در سطح معنی‌داری (0/05 > P) انجام شد.
یافته‌ها: فعالیت در محیط غنی‌شده و مصرف سینئول به‌طور مستقل و تعاملی باعث افزایش بیان این ژن‌ها گردید (0/001 P <). مسیر NRF2/SOD1/HMOX1 در گروه چاق با رژیم پرچرب حاوی آکریل‌آمید بطور معنی‌داری نسبت به سایر گروه‌ها کاهش یافت (0/001 = P). مصرف سینئول و فعالیت در محیط غنی شده سبب افزایش معنی‌دار بیان ژن‌های گردید (0/001 = P).
نتیجه‌گیری: این یافته‌ها بیانگر نقش محافظتی سینئول و فعالیت در تعدیل استرس اکسیداتیو و ارتقای ظرفیت آنتی‌اکسیدانی است. این دو مداخله می‌تواند به‌عنوان رویکردی مؤثر در پیشگیری از اختلالات متابولیکی مرتبط با چاقی مطرح شود.

تازه های تحقیق

فرزانه تقیان: Google Scholar 

خسرو جلالی دهکردی: Google Scholar 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Modulation of the Nrf1/Sod1/Hmox1 Pathway by Cineole Consumption and Exercise in an Enriched Environment Protects the Liver Against Acrylamide Toxicity

نویسندگان [English]

  • Masoud Ardeshirilordejani 1
  • Farzaneh Taghian 2
  • Khosro Jalali Dehkordi 3
1 Department of Sports Physiology, Isf.C., Islamic Azad University, Isfahan, Iran
2 Professor, Department of Sports Physiology, Isf.C., Islamic Azad University, Isfahan, Iran
3 Associate Professor, Department of Sports Physiology, Isf.C., Islamic Azad University, Isfahan, Iran
چکیده [English]

Background: Oxidative stress caused by obesity and exposure to acrylamide reduces the ability of antioxidant defenses. This study evaluated the effects of a combined intervention of exercise in an enriched environment and a cineole supplement on the modulation of gene expression in the Nrf2/SOD1/HMOX1 pathway in obese mice induced by a high-fat diet.
Methods: The present study is an experimental, basic study. Fifty-four 6–7-week-old female C57BL/6 mice were randomly divided into 9 groups: control, obese with a high-fat diet containing acrylamide, obese with a high-fat diet without acrylamide, obese with an acrylamide diet and exercise in an enriched environment (8 weeks, 5 days/week, 60–90 min daily, low-moderate intensity), obese with an acrylamide diet and cineole (10 μM administered by oral gavage), obese with an enriched environment, obese with cineole, obese with an acrylamide diet combined with an enriched environment and cineole, and obese with an enriched environment and cineole. The expression of Nrf2/SOD1/HMOX1 genes in the liver was examined by Real-Time qPCR. Data were analyzed via one-way and three-way ANOVA at a significance level of P < 0.05.
Findings: Both enriched environment exercise and cineole administration, individually and in combination, significantly increased the expression of these genes (P < 0.001). The Nrf2/SOD1/HMOX1 pathway was markedly downregulated in obese mice receiving a high-fat diet containing acrylamide compared to the other groups (P = 0.001). In contrast, cineole treatment and enriched environment exercise induced a significant upregulation of these antioxidant genes (P = 0.001).
Conclusion: These findings indicate the protective role of cineole and physical activity in modulating oxidative stress and enhancing antioxidant capacity. These two interventions can be proposed as an effective approach in preventing metabolic disorders associated with obesity.

کلیدواژه‌ها [English]

  • Obesity
  • Physical activity
  • Oxidative stress
  • Nrf2
  • Superoxide dismutase 1
  • 1,8-Cineole

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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 833
هفته 1، آذر
آذر و دی 1404
صفحه 1228-1240

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  • تاریخ دریافت: 16 آذر 1404
  • تاریخ پذیرش: 30 آذر 1404

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