اثر 8 هفته تمرین هوازی تناوبی قبل و بعد از القای سرطان پستان بر سطح سرمی آیریسین و رشد تومور در موش Balb/c

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

2 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

3 استاد، گروه تریبت بدنی، دانشکده‌ی علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

4 دانشجوی دکتری، گروه سلولی- مولکولی، دانشکده‌ی علوم پایه، دانشگاه آزاد علوم و تحقیقات، تهران، ایران

5 مرکز تحقیقات سرطان، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

مقدمه: آیریسین مایوکاینی است که می‌تواند به عنوان یک نشانگر جدید، ارتباط بین ورزش و کاهش شیوع و پیشرفت سرطان را نشان دهد. هدف از انجام این مطالعه، بررسی اثر 8 هفته تمرین هوازی تناوبی قبل و بعد از القای سرطان پستان بر سطح سرمی آیریسین و رشد تومور در موش Balb/c بود.روش‌ها: در این تحقیق نیمه‌تجربی، 40 سر موش آزمایشگاهی نژاد Balb/c ماده، به روش تصادفی در 4 گروه شاهد، تومور، ورزش و ورزش به همراه تومور قرار گرفتند. تمرین اصلی، 4 هفته قبل ‌و ‌4 ‌هفته ‌بعد از ایجاد تومور با شدت‌های 70 و 50 درصد حداکثر اکسیژن مصرفی (Maximum rate of oxygen consumption یا VO2max) اجرا گردید. موش‌های گروه تومور و گروه ورزش به همراه تومور، از طریق جراحی زیر جلدی با تومور آدنوکارسینومای پستان موشی، مبتلا به سرطان شدند. رشد تومور هر هفته اندازه‌گیری گردید. در پایان مطالعه، تومورها با جراحی برداشته شدند. میزان آیریسین سرم، با روش Enzyme-linked immunosorbent assay (ELISA) اندازه‌گیری شد. برای بررسی بیان ژن Vascular endothelial growth factor (VEGF) از روش Real-time polymerase chain reaction (Real-time PCR) استفاده شد. داده‌ها با استفاده از آزمون t و ضریب همبستگی Pearson در سطح معنی‌داری 05/0 > P تجزیه و تحلیل شدند.یافته‌ها: کاهش معنی‌داری در میزان رشد تومور در گروه ورزش به همراه تومور نسبت به گروه تومور مشاهده گردید. سطح سرمی آیریسین به ‌طور معنی‌داری در گروه ورزش و ورزش به همراه تومور، به ترتیب نسبت به گروه شاهد و گروه توموری افزایش یافت. میزان بیان VEGF در گروه ورزش به همراه تومور نسبت به گروه تومور کاهش معنی‌داری نشان داد (003/0 = P). ارتباط معنی‌داری بین سطوح آیریسین با نسبت رشد تومور درگروه ورزش به همراه تومور مشاهده شد (001/0 > P، 916/0- = r).نتیجه‌گیری: به نظر می‌رسد که نقش مثبت تمرینات منظم هوازی در کاهش رشد سلول‌های سرطانی پستان موش‌های Balb/c از طریق افزایش سطح آیریسین و کاهش بیان VEGF، باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 8 Weeks of Interval Aerobic Exercise before and after Induction of Breast Cancer on Serum Level of Irisin and Tumor Growth in Balb/c mice

نویسندگان [English]

  • Samaneh Khalighfard 1
  • Hamid Rajabi 2
  • Reza Gharakhanlou 3
  • Solmaz Khalighfard 4
  • Vahid Setoudeh 1
  • Ali Mohammad Alizadeh 5
1 PhD Student, Department of Physiology, School of Physical Education and Sports Sciences, Kharazmi University, Tehran, Iran
2 Professor, Department of Physiology, School of Physical Education and Sports Sciences, Kharazmi University, Tehran, Iran
3 Professor, Department of Physical Education, School of Humanities, Tarbiat Modares University, Tehran, Iran
4 PhD Student, Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
چکیده [English]

Background: Irisin is a myokine, can be used as a new indicator of relationship between exercise and reduction of incidence and progression of cancer. The aim of this study was to investigate the effect of 8 weeks of interval aerobic exercise before and after induction of breast cancer on serum level of irisin and tumor growth in Balb/c mice.Methods: In this quasi-experimental study, 40 adult female Balb/c mice were randomly divided into four groups of control, tumor, exercise, and exercise with tumor. The main training with 70% and 50% of the maximum rate of oxygen consumption (VO2max) intensity was performed 4 weeks before and 4 weeks after tumor induction. Tumor and exercise with tumor groups were cancerous with murine breast tumors of adenocarcinoma through subcutaneous surgery. Tumor growth was measured weekly, and at the end of the study, tumors were surgically removed. Serum irisin was measured using enzyme-linked immuonosorbent assay (ELISA) method. Real-time polymerase chain reaction (Real-time PCR) was used to investigate the expression of vascular endothelial growth factor (VEGF) gene. Data were analyzed using Student’s t and Pearson correlation tests at the significance level of P < 0.05.Findings: There was a significant decrease in tumor growth rate in exercise group with tumor compared to tumor group. Serum levels of irisin increased significantly in exercise and exercise with tumor groups compared to control and tumor groups, respectively. The expression of vascular endothelial growth factor in exercise with tumor group showed a significant decrease compared to tumor group (P = 0.003). There was a significant correlation between irisin levels and tumor growth ratio in exercise with tumor group (P < 0.001, r = -0.9162).Conclusion: It seems that positive regular aerobic exercises can reduce the growth of breast cancer cells in Balb/c mice by increasing the irisin level and decreasing expression of vascular endothelial growth factor.

کلیدواژه‌ها [English]

  • Aerobic Exercise
  • Irisin
  • Breast Cancer
  • Balb/c mice
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