Effect of Cholesterol, Iron and Vitamin E on Protamine Deficiency and DNA Fragmentation of Male Rabbit Sperm

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine AND Student Research Committee, Isfahan University of medical sciences, Isfahan, Iran

2 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Senior Demonstrator, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 MSc Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The role of vitamin E as an antioxidant and iron-containing compounds on DNA is not well defined. So, recent study was carried out to determine the role of vitamin E and iron, as both separate and combined effect on sperm protamine deficiency and DNA fragmentation in male rabbits.Methods: This study included 42 male rabbits which were randomly divided into seven groups each containing six rabbits.They were all treated under special diets for 6 weeks. After this period the animals were anesthetized and sacrificed .The epididymis duct and vas deferens were dissected and their sperms were extracted from it by Ham's f10 solution. Sperm DNA fragmentation and protamine deficiency were evaluated by the acridine orange and chromomycin A3 staining, respectively.Findings: The mean values of protamine deficiency and DNA fragmentation were significantly higher in group that concomitant use of cholesterol and iron. In addition, Vitamin E as an antioxidant agent has a significant effect in reducing these damages.Conclusion: Vitamin E as an antioxidant, decrease sperm DNA damage resulting from the consumption of foods high in cholesterol and iron.

Keywords

References

  1. Balhorn R. A model for the structure of chromatin in mammalian sperm. J Cell Biol 1982; 93(2): 298-305.
  2. Sakkas D, Urner F, Bianchi PG, Bizzaro D, Wagner I, Jaquenoud N, et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 1996; 11(4): 837-43.
  3. Marushige Y, Marushige K. Enzymatic unpacking of bull sperm chromatin. Biochim Biophys Acta 1975; 403(1): 180-91.
  4. Auger J, Mesbah M, Huber C, Dadoune JP. Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men. Int J Androl 1990; 13(6): 452-62.
  5. Iranpour FG, Nasr-Esfahani MH, Valojerdi MR, al-Taraihi TM. Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet 2000; 17(1): 60-6.
  6. Nasr-Esfahani MH, Abasi H, Razavi S, Ashrafi S, Tavalaee M. Varicocelectomy: semen parameters and protamine deficiency. Int J Androl 2009; 32(2): 115-22.
  7. Pogany GC, Corzett M, Weston S, Balhorn R. DNA and protein content of mouse sperm. Implications regarding sperm chromatin structure. Exp Cell Res 1981; 136(1): 127-36.
  8. Kemal DN, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 2000; 74(6): 1200-7.
  9. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9(4): 331-45.
  10. Halliwell B, Gutteridge JM. Free radicals in biology and medicine. 3rd ed. New York, NY: Oxford University Press; 1999. p. 617–783.
  11. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002; 29(4): 817-27.
  12. Hull MG, North K, Taylor H, Farrow A, Ford WC. Delayed conception and active and passive smoking. The Avon Longitudinal Study of Pregnancy and Childhood Study Team. Fertil Steril 2000; 74(4): 725-33.
  13. Suleiman SA, Ali ME, Zaki ZM, el-Malik EM, Nasr MA. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl 1996; 17(5): 530-7.
  14. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003; 49(2): 83-94.
  15. Chaler Jea SN. Clin chemistry. New Delhi, India: Jaypee Publishers: Medical Publications; 1999.
  16. Aitken RJ, Buckingham DW, West KM. Reactive oxygen species and human spermatozoa: analysis of the cellular mechanisms involved in luminol- and lucigenin-dependent chemiluminescence. J Cell Physiol 1992; 151(3): 466-77.
  17. Wellejus A, Poulsen HE, Loft S. Iron-induced oxidative DNA damage in rat sperm cells in vivo and in vitro. Free Radic Res 2000; 32(1): 75-83.
  18. Lucesoli F, Caligiuri M, Roberti MF, Perazzo JC, Fraga CG. Dose-dependent increase of oxidative damage in the testes of rats subjected to acute iron overload. Arch Biochem Biophys 1999; 372(1): 37-43.
  19. Perera D, Pizzey A, Campbell A, Katz M, Porter J, Petrou M, et al. Sperm DNA damage in potentially fertile homozygous beta-thalassaemia patients with iron overload. Hum Reprod 2002; 17(7): 1820-5.
  20. Corona G, Mannucci E, Schulman C, Petrone L, Mansani R, Cilotti A, et al. Psychobiologic correlates of the metabolic syndrome and associated sexual dysfunction. Eur Urol 2006; 50(3): 595-604.
  21. Demir T, Demir O, Kefi A, Comlekci A, Yesil S, Esen A. Prevalence of erectile dysfunction in patients with metabolic syndrome. Int J Urol 2006; 13(4): 385-8.
  22. Kasturi SS, Tannir J, Brannigan RE. The metabolic syndrome and male infertility. J Androl 2008; 29(3): 251-9.
  23. Pasquali R. Obesity, fat distribution and infertility. Maturitas 2006; 54(4): 363-71.
  24. Pasquali R, Patton L, Gambineri A. Obesity and infertility. Curr Opin Endocrinol Diabetes Obes 2007; 14(6): 482-7.
  25. Hammoud AO, Gibson M, Peterson CM, Hamilton BD, Carrell DT. Obesity and male reproductive potential. J Androl 2006; 27(5): 619-26.
  26. Traber MG, Packer L. Vitamin E: beyond antioxidant function. Am J Clin Nutr 1995; 62(6 Suppl): 1501S-9S.
  27. Ward WS, Coffey DS. DNA packaging and organization in mammalian spermatozoa: comparison with somatic cells. Biol Reprod 1991; 44(4): 569-74.
Journal of Isfahan Medical School
Volume 31, Issue 259 - Serial Number 259
September and October 2014
Pages 1769-1778

Files

History

  • Receive Date: 11 June 2013
  • Revise Date: 23 May 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics