دوره 31، شماره 232: هفته دوم خردادماه 1392:439-455

تأثیر میدان مغناطیسی كم فرکانس بر سرعت رشد و تکثیر سلول‌های بنیادی مزانشیمی مشتق از بافت چربی انسانی

مرضیه سلیمی, داریوش شهبازی گهرویی, سعید کرباسی, سعید کرمانی, شهناز رضوی

چکیده


مقدمه: سلول‌های بنیادی به دلیل قابلیت تکثیر و تمایز به سلول‌های دیگر مدل خوبی برای بررسی اثرات میدان مغناطیسی كم فرکانس (Extremely low-frequency electromagnetic field یا ELF-EMF) بر سلول‌های بدن می‌باشند. بافت چربی به عنوان منبعی از سلول‌های بنیادی مزانشیمی شناخته می‌شود. در این مطالعه تأثیر تابش با شدت‌های مختلف بر میزان تکثیر بر سلول‌های بنیادی مزانشیمی مشتق‌شده از بافت چربی انسانی در زمان‌های تابش20 و 40 دقیقه بررسی شد.

روش‌ها: در این مطالعه تأثیر تابش با شدت 5/0 و 1 میلی‌تسلا و فرکانس 50 هرتز بر میزان تکثیر سلول‌های بنیادی مزانشیمی مشتق‌شده از بافت چربی انسانی با زمان‌های تابش20 و 40 دقیقه در روز به مدت 7 روز متوالی بررسی گردید. از تکنیک MTT assay برای بررسی رشد و میزان بقای سلول‌ها و از رنگ‌آمیزی تریپان بلو به منظور ارزیابی میزان تکثیر سلول‌ها استفاده گردید. آنالیز داده‌ها با استفاده از آزمون One way ANOVA انجام شد.

یافته‌ها: میزان تکثیر و بقای سلول‌ها در همه‌ی گروه‌های تابش به طور معنی‌داری بیشتر از گروه‌های شاهد بود (05/0 > P) و تنها گروه 1 میلی‌تسلا به مدت 40 دقیقه در روز، اختلاف معنی‌داری با گروه شاهد نداشت.

نتیجه‌گیری: نتایج مطالعه‌ی حاضر نشان داد که تابش میدان مغناطیسی با شدت‌های 5/0 و 1 میلی‌تسلا با توجه به زمان تابش می‌تواند باعث تحریک رشد و تکثیر سلول‌ها شود. مکانیسم این تأثیر هنوز ناشناخته می‌باشد.


واژگان کلیدی


ميدان مغناطيسي كم فرکانس؛ سلول‌هاي بنیادی مزانشيمي؛ چربی؛ سرعت تکثیر و رشد

تمام متن:

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