دوره 31، شماره 268: هفته دوم اسفند ماه 1392:2238-2249

بررسی برون تنی عصاره‌ی هیدروالکلی سیر وحشی (Allium ampeloprasum) بر فعالیت حرکتی ایلئوم موش صحرایی

مهرنوش صدیقی هفشجانی, مصیب نوری احمدآبادی, حمید نصری, ملوک هادی, محمود رفیعیان کوپائی

چکیده


مقدمه: در این تحقیق، اثر ضد انقباضی عصاره‌ی هیدروالکلی برگ گیاه تره‌ی کوهی بر انقباضات ایلئوم موش‌های صحرایی نژاد ویستار و مکانیسم محتمل بر آن بررسی شد.

روش‌ها: در این تحقیق مداخله‌ای تعداد 48 سر موش صحرایی، از نژاد ویستار به طور تصادفی به شش گروه هشت‌تایی به شرح زیر تقسیم شدند: شاهد، دریافت کننده‌ی غلظت‌های تجمعی عصاره‌ی تره‌ی کوهی، دریافت کننده‌ی L-NAME (L-NG-nitroarginine methyl ester) و عصاره، نالوکسان و عصاره، پروپرانولول و عصاره و گروه دریافت کننده‌ی غلظت‌های تجمعی کلرور کلسیم. در روز آزمایش، ایلئوم موش‌ها جدا و انقباضات آن در حمام بافت تحت یك گرم كشش با اضافه کردن كلرور پتاسیم (mM 60) به روش ایزوتونیك ثبت شد. جهت بررسی مکانیسم اثر عصاره، بافت با پروپرانول، نالوکسان و یا L-NAME انکوبه شد و درصد تغییرات نیروی انقباضی بر روی کاغذ ثبت و محاسبه گردید. همچنین جهت نقش کانال‌های کلسیم در فعالیت حرکتی بافت، ایلئوم تحت تأثیر غلظت‌های تجمعی کلرور کلسیم قرار داده شد.

یافته‌ها: عصاره‌ی تجمعی تره‌ی کوهی (100، 200 و 400 میلی‌گرم بر کیلوگرم) به صورت وابسته به غلظت انقباضات ایلئوم به وسیله‌ی کلرور پتاسیم را کاهش داد (0001/0 > P). پروپرانولول اثر مهاری عصاره بر انقباضات ناشی از کلرور پتاسیم را به صورت معنی‌داری کاهش داد (0010/0 > P). اما، L-NAME و نالوکسان سبب کاهش اثر مهاری عصاره بر ایلئوم نشدند. همچنین کلسیم سبب انقباض بافت دپولاریزه شده توسط کلرور پتاسیم شد و این اثر انقباضی توسط غلظت‌های تجمعی عصاره به طور معنی‌داری کاهش یافت (0010/0 > P).

نتیجه‌گیری: عصاره‌ی هیدروالکلی برگ تره‌ی کوهی توانست با اثر بر گیرنده‌های بتاآدرنرژیک و کانال‌های کلسیم وابسته به ولتاژ، بر فعالیت حرکتی ایلئوم موش صحرایی اثر بگذارد و با توجه به نتیجه‌ی اثر، ممکن است بتوان از آن در درمان ناراحتی‌های گوارش استفاده کرد.


واژگان کلیدی


عصاره‌ی تره‌ی کوهی؛ پروپرانولول؛ ایلئوم؛ موش صحرایی

تمام متن:

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