Study of SFRP1 and SFRP2 Genes Methylation Status in Patients with De Novo Acute Myeloblastic Leukemia

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

2 Hematology-Oncology and Stem cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: Acute myeloid leukemia (AML) is a heterogeneous group of hematologic malignancies with great variability in the pathogenesis and clinical course. DNA methylation of CpG islands within the promoters of specific genes may play roles in tumor initiation and progression. Secreted frizzled-related proteins (SFRPs) are negative regulator of the Wnt signaling pathway. In the present study, we compared the methylation status of SFRP1 and SFRP2 genes promoter in patients with AML and healthy individuals.Methods: In peripheral blood from 43 patients afflicted with de novo AML and 25 healthy controls, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction (MSP) with primers specific for methylated and unmethylated promoter sequences of the SFRP1 and SFRP2 genes. We used Mann-Whitney u-tests to investigate the correlation between SFRP1 and SFRP2 genes hypermethylation and clinical parameters.Findings: The frequency of aberrant hypermethylation of SFRP1 and SFRP2 genes promoter in patients with AML was determined 30.2% (13/43) and 20.9% (9/43), respectively. In addition, for all subjects in control group, methylation of SFRP1 and SFRP2 genes promoter were negative. Patients with M0 subtype of FAB-AML had the highest incidence of hypermethylation of SFRP1 (P = 0.028) and SFRP2 (P = 0.004) genes promoter.Conclusion: The present study showed that, like many solid tumors, SFRPs genes methylation also occurs in AML. Therefore, the methylation of these genes may play a role in leukemogenesis initiation.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 286 - Serial Number 286
March and April 2014
Pages 702-711

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History

  • Receive Date: 27 February 2014
  • Revise Date:
  • Accept Date: 06 April 2022

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