Effects of DAPT on Serum Vascular Endothelial Growth Factor and Its Soluble Receptor in Obese BALB/C Mice

Document Type : Original Article (s)

Authors

1 Student of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

2 PhD Student, Department of Physiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Today, obesity is considered as a risk factor for tumor growth. Angiogenesis is involved in physiological conditions like wound repair and pathological states such as tumor growth. Gamma-secretase is an enzyme that is involved during angiogenesis process. This study aimed to investigate the effects of DAPT on serum vascular endothelial growth factor (VEGF) and its soluble receptor (VEGFR1) in obese BALB/c mice. Methods: A total number of 12 BALB/c mice were purchased from Pasteur Institute (Tehran, Iran). Obesity was induced by feeding the mice on a high-fat diet (HFD) for 20 weeks. The animals were then randomized into two groups of HFD and HFD plus DAPT. In the second group, 10 mg/kg/day of subcutaneous DAPT was administered for 14 days. Afterward, blood samples were obtained and serum VEGF and VEGFR1 levels were measured. Body weights were measured every week. Findings: DAPT could not significantly change body weight. Moreover, the two groups were not significantly different in terms of serum VEGF concentrations. However, serum VEGFR1 levels were significantly lower in mice receiving DAPT than in the HFD group. Conclusion: Since DAPT alters serum VEGFR1, it may be involved during angiogenesis and inhibition of tumor growth. Keywords: Obesity, Vascular endothelial growth factor, High-fat diet
Journal of Isfahan Medical School
Volume 30, Issue 214 - Serial Number 214
September and October 2013
Pages 1942-1948

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History

  • Receive Date: 28 November 2012
  • Revise Date: 01 June 2024
  • Accept Date: 06 April 2022

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