The Sperm and In-Vitro Fertilization (IVF) of Mice with Phenylhydrazine-Induced Hemolytic Anemia; Ameliorating the Effect of Royal Jelly and Vitamin C

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Basic Sciences, School of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Associate Professor, Department of Basic Sciences, School of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Assistant Professor, Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran

4 Professor, Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran

5 PhD Student, Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia , Iran

Abstract

Background: Hemolytic anemia induced by phenylhydrazine (PHZ), as a hemolytic compound, can cause disorders in spermatogenesis and fertility. The present study aimed to evaluate the effect of vitamin C (Vit C) and royal jelly (RJ) as effective antioxidant compounds against phenylhydrazine-induced hemolytic anemia.Methods: Adult male mice were randomly diveded into four groups of eight. The first group received 0.1 ml of normal saline intraperitonealy (IP). The second group received 60 mg/kg of phenylhydrazine in 48-hour intervals intraperitonealy. The third group received 60 mg/kg of phenylhydrazine along with 250 mg/kg of vitamin C intraperitonealy and100 mg/kg of royal jelly orally. The fourth group received the same doses of vitamin C and royal jelly as the third group. After 35 days, serum samples were taken, the sperms were collected from epididymis and in-vitro fertilization (IVF) was evaluated.Findings: Phenylhydrazine-induced hemolytic anemia significantly decreased serum testosterone concentration and increased damaged DNA and immature nuclear sperm. Moreover, phenylhydrazine decreased the number of fertilized oocytes, two-cell and four-cell embryos, morula and blastocyst and increased arrested embryos. However, administrating of royal jelly and vitamin C improved these parameters significantly.Conclusion: It seems that royal jelly and vitamin C, as free radicals scavengers, have the potential to decrease oxidative damages on reproductive organ in phenylhydrazine-induced hemolytic anemia.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 363 - Serial Number 363
November and December 2016
Pages 2193-2203

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History

  • Receive Date: 22 September 2015
  • Revise Date:
  • Accept Date: 06 April 2022

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