اثر فعالیت شنای اختیاری بر محتوای پروتئین‌های iNOS و COX-2 ، حافظه و عملکرد حرکتی رت‌های مدل مالتیپل اسکلروزیس القا شده با کوپریزون

نوع مقاله : Original Article(s)

نویسندگان

1 کارشناسی ارشد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 استادیار، واحد تحقیقات سلامت از طریق ورزش و زندگی فعال (HEAL)، گروه علوم زیست پزشکی، دانشکده‌ی پزشکی و علوم بهداشتی، دانشگاه صباح مالزی، جالان UMS، کوتا کینابالو، صباح، مالزی

10.48305/jims.v41.i748.1118

چکیده

مقاله پژوهشی




مقدمه: مالتیپل اسکلروزیس (Multiple sclerosis) MS، یکی از بیماری‌های التهابی مزمن، با واسطه‌گری دستگاه ایمنی، در دستگاه عصبی مرکزی است. از آن‌جایی‌که فعالیت بدنی دارای اثرات ضد التهابی و ضد اکسایشی است، لذا هدف مطالعه‌ی حاضر، بررسی اثر فعالیت شنای اختیاری بر محتوای پروتئین‌های iNOS و COX-2 و عملکرد شناختی و حرکتی رت‌های مدل MS می‌باشد.
روش‌ها: در این مطالعه، تعداد 20 سر رت با میانگین سن 12 هفته به چهار گروه شاهد سالم، شاهد بیمار، سالم تمرین و تمرین بیمار تقسیم شدند. به منظور القاء MS، رت‌های گروه‌های بیمار در تمام 12 هفته طول پروتکل تحقیق با غذای محتوی کوپریزون 0/5 درصد تغذیه شدند. پس از پایان 6 هفته پروتکل تمرینی و انجام آزمون‌های رفتاری، بافت هیپوکمپ استخراج و مقادیر پروتئین‌ها با استفاده از روش وسترن بلات اندازه‌گیری شد.
یافته‌ها: نتایج آزمون‌های حافظه و عملکرد حرکتی نشان داد که عملکرد حافظه و فعالیت حرکتی و حفظ تعادل در گروه بیمار دچار اختلال شده و متعاقب یک دوره فعالیت شنا بهبود قابل توجهی یافت. همچنین نتایج نشان داد که محتوای پروتئین‌های iNOS و COX-2 در گروه شاهد بیمار به صورت معنی‌دار نسبت به گروه شاهد سالم افزایش یافت، همچنین مقادیر این پروتئین‌ها در گروه تمرین بیمار نسبت به گروه شاهد بیمار به صورت قابل توجهی کاهش پیدا کرد.
نتیجه‌گیری: بر اساس نتایج مطالعه‌ی حاضر، فعالیت ورزشی شنای اختیاری احتمالاً می‌تواند منجر به حفاظت بیشتری از عصب و ارتقاء عملکرد حافظه و فعالیت حرکتی و تعادل بیماران مبتلا به MS گردد و به عنوان یک راهبرد غیر دارویی امیدوارکننده باشد.

تازه های تحقیق

سید شفا مرعشی: Google Scholar

محمد رمی: PubMed ,Google Scholar 

سعید شاکریان:  PubMed ,Google Scholar 

دی ماریاما آوانگ داود: PubMed ,Google Scholar 

 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effects of Voluntary Swimming on iNOS and COX-2 Protein Contents as Well as Memory and Motor Performance in Cuprizone-Induced Multiple Sclerosis Rats Model

نویسندگان [English]

  • Sayed Shafa Marashi 1
  • Mohammad Rami 2
  • Saeed Shakeryan 3
  • Maryama Awang Daud 4
1 MSc, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Assistant Professor, Health Through Exercise and Active Living (HEAL) Research Unit, Department of Biomedical Sciences, School of Medicine and Health Sciences, University Malaysia Sabah, Kota Kinabalu 88400, Jalan UMS, Kota Kinabalu 88450, Sabah, Malaysia
چکیده [English]

Background: Multiple sclerosis (MS) is a chronic inflammatory disease that affects the central nervous system and is mediated by the immune system. Since physical activity has anti-inflammatory and anti-oxidative effects, Therefore, this study aims to investigate the effect of voluntary swimming activity on the iNOS & COX-2 protein content, as well as the cognitive and motor performance of the Multiple Sclerosis rats model.
Methods: In the present study, 20 rats with an average age of 12 weeks were categorized into four groups, including healthy control, MS control, healthy training, and MS training. To induce MS, the rats of the MS groups were fed food infused with 0.5%cuprizone throughout the entire 12-week research protocol. After the end of 6 weeks of training protocol and conducting behavioral tests, the hippocampus tissue was extracted, and the number of proteins was measured using the western blot method.
Findings: The results of the memory and motor function tests revealed that subjects with MS had impaired memory function, motor activity, and difficulty maintaining balance. However, following a period of swimming activity, these functions significantly improved. The results also showed that the levels of iNOS and COX-2 proteins in the MS control group were significantly elevated compared to the healthy control group. On the other hand, the MS training group showed significant reductions in the levels of these proteins compared to the MS control group.
Conclusion: Based on the results of the current research, voluntary swimming exercise activity could potentially lead to more nerve protection and improvement of memory function, motor activity, and balance in patients with MS and can be promising as a non-pharmacological treatment strategy.

کلیدواژه‌ها [English]

  • Swimming
  • Multiple sclerosis
  • Hippocampus
  • Exercise

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 748
هفته 4، بهمن
بهمن و اسفند 1402
صفحه 1118-1127

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