بررسی فراوانی پلی‌مورفیسم rs4680 ژن COMT در عملکرد رقابتی ورزشکاران نخبه‌ی رزمی‌کار زن و مرد ایرانی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده‌ی ادبیات و علوم انسانی، گروه تربیت بدنی، دانشگاه لرستان، لرستان، ایران

2 استاد، گروه تربیت بدنی و علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران

3 دانشیار، گروه تربیت بدنی و علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران

4 استادیار، گروه زیست‌شناسی، دانشکده‌ی علوم پایه، دانشگاه لرستان، لرستان، ایران

چکیده

مقاله پژوهشی




مقدمه: ژن COMT می‌تواند بر خواص و فعالیت آنزیم‌ها، سطح دوپامین، توانایی شناختی و در نتیجه در کنترل و اجرای بهتر در ورزش نقش مهمی بازی کند. هدف پژوهش حاضر، بررسی فراوانی پلی‌مورفیسم rs4680 ژن COMT در عملکرد رقابتی ورزشکاران نخبه‌ی رزمی‌کار زن و مرد ایرانی بود.
روش‌ها: آزمودنی‌های شامل 45 رزمی‌کار مرد (20 کشتی‌گیر، 20 تکواندوکار و 5 جودوکار) با میانگین سنی (7/18 ± 22/48)، 40 رزمی‌کار زن (16 وشوکار،
4 جودوکار و 20 تکواندوکار) با میانگین سنی (7/13 ± 19/11) و 60 غیر ورزشکار با میانگین سنی (73/20 ± 20/15) بودند، استخراج DNA از نمونه‌ی بزاق انجام گرفت. برای تعیین ژنوتیپ، از روش Tetra ARMS PCR استفاده شد، با استفاده از سرور RNAsnp و PolyPhen-2 اثر جهش‌ها بر روی ساختار دوم mRNA و عملکرد ژن COMT بررسی گردید. از آزمون Chi-square جهت بررسی فراوانی ژنوتیپ‌ها استفاده شد.
یافته‌ها: در بررسی ژنوتیپ‌های ژن COMT فراوانی ژنوتیپ GA در گروه مردان و زنان نخبه‌ی رزمی‌کار به ترتیب 25 و 27/5 درصد از گروه غیر ورزشکار بیشتر شد که این اختلاف معنی‌دار بود و همچنین در مجموع آلل A در گروه نخبه‌ی زن و مرد 23/75درصد بیشتر از گروه شاهد و اختلاف نیز معنی‌دار شد.
نتیجه‌گیری: به نظر می‌رسد که ارتباط مثبتی میان ژنوتیپ GA و آلل A پلی‌مورفیسم ژن COMT با نخبه شدن در ورزش‌های رزمی وجود دارد که می‌توان به ‌عنوان یکی از عوامل ژنتیکی مؤثر در موفقیت افراد رزمی‌کار در سطح حرفه‌ای به‌حساب آورد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Examining the Frequency of COMT Gene rs4680 Polymorphism Iranian Male and Female Elite Combat Athletes

نویسندگان [English]

  • Vahid Taeid 1
  • Massoud Rahmati 2
  • Muhammad Fathi 3
  • Faranak Hadi 4
1 PhD Student in Sports Physiology, School of Literature and Humanities, Department of Physical Education, Lorestan University, Lorestan, Iran
2 Professor, Department of Physical Education and Sport Sciences, School of Literature and Humanities, Lorestan University, Lorestan, Iran
3 Assistant Professor, Department of Physical Education and Sport Sciences, School of Literature and Humanities, Lorestan University, Lorestan, Iran
4 Assistant Professor, Department of Biology, School of Basic Sciences, Lorestan University, Lorestan, Iran
چکیده [English]

Background: COMT gene can play an important role on the properties and activity of enzymes, dopamine level, cognitive ability and as a result in better control and performance in sports, the aim of the present study is to investigate the frequency of rs4680 polymorphism of COMT gene in the competitive performance of Iranian male and female martial arts athletes.
Methods: The subjects included 45 male martial artists (20 wrestlers, 20 taekwondo players and 5 judo players) with an average age (22.48 ± 7.18), 40 female martial artists (16 wrestlers, 4 judo players and 20 taekwondo players) with an average age (11 ± 7.13) 19) and 60 were non-athletes with an average age of (20.15 ± 73.20), DNA extraction was done from saliva samples. Tetra ARMS PCR method was used to determine the genotype, using RNAsnp and PolyPhen-2 server, the effect of mutations on the second structure of mRNA and COMT gene function was investigated. Chi-square test was used to check the frequency of genotypes.
Findings: In the analysis of COMT gene genotypes, the frequency of GA genotype in elite martial arts men and women was 25% and 27.5%, respectively, more than the non-athletes group, which was a significant difference; Also, in total, the A allele in the elite group of men and women was 23.75% more than the control group and the difference was also significant.
Conclusion: It seems that there is a positive relationship between the GA genotype and the A allele of the COMT gene polymorphism with being elite in martial arts, which can be considered as one of the genetic factors that influence the success of martial artists at the professional level.

کلیدواژه‌ها [English]

  • Single nucleotide polymorphism
  • Allele frequency
  • Athletes
  • COMT protein
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