In-Vitro Study of Homocysteine and Aspirin Effects on Coagulation and Clot Permeability

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

3 Associate Professor, Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Department of Biochemistry, School of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Abstract

Background: Some studies suggest that increased homocystein in blood leads to alterations in coagulation; however, the precise mechanism is not clear. The aim of this study was to compare different concentrations of homocysteine and aspirin on coagulation parameters and permeability coefficient of clot in the plasma of healthy individuals in vitro.Methods: Different concentrations of homocysteine (50, 100, 200 µM) and aspirin (1, 10, 100 mg/l) were added to the plasma citrate. They were incubated at 37°C for 24 hours. Then, coagulation parameters were analyzed by the turbidimetric procedure at 405 nm and permeability coefficient of clot was determined. The independent samples t-test was utilized to compare the results.Findings: Homocysteine at 200 µM with aspirin 100 mg/l had significant changes in the total coagulation time (820.00 ± 1.15 s), maximum coagulation velocity (0.232 ± 0.003), the permeability coefficient of clot [(1.108 × 10-6) ± 0.151] compared to homocysteine at 200 µM [555.00 ± 12.89, 0.258 ± 0.008, (0.493 × 10-6) ± 0.038, respectively] (P < 0.05). Homocysteine at 200 µM with aspirin 1 mg/l did not significantly change in either parameter (P > 0.05). Homocysteine at 100 µM with aspirin 1 mg/l significantly changed only in the permeability coefficient [(0.787 × 10-6) ± 0.071] compared to homocysteine at 100 µM [(0.636 × 10-6) ± 0.053] (P < 0.05).Conclusion: Aspirin with the dose more than 1 mg/l had more effect on higher concentrations of homocysteine and delayed forming clot by impeding the coagulation. It increased clot permeability, too.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 262 - Serial Number 262
September and October 2014
Pages 1932-1940

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History

  • Receive Date: 01 December 2013
  • Revise Date: 23 May 2024
  • Accept Date: 06 April 2022

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