Effect of Acute Resistance Training on Plasma Irisin Protein Level and Expression of Muscle FNDC5 and Adipose Tissue UCP1 Genes in Male Rats

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Exercise Physiology, School of Physical Education, Kharazmi University, Tehran, Iran

2 Associate Professor, Department of Exercise Physiology, School of Physical Education, Kharazmi University, Tehran, Iran

3 Associate Professor, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran

4 Associate Professor, Department of Exercise Physiology, School of Physical Education, University of Isfahan, Isfahan, Iran

Abstract

Background: Change in adipose tissue phenotype caused by exercise training is the new theory raised recently. However, the identification of cellular and molecular mechanisms by which exercise training exert its benefits are being investigated. The purpose of this study was to determine the effect of resistance training on plasma irisin protein level and expression of soleus muscle FNDC5 and subcutaneous adipose tissue UCP1 genes in male rats.Methods: 16 Sprague dolly male rats (mean weight = 221.00 ± 2.31 g; age: 8 weeks) divided in 2 groups, resistance training and control, randomly. Training group exercised on a special ladder (1.2 meters height) and carried a weight with 50% of their body weight, which was closed to their tails, 3 sets of 5 reps with 3 minutes rest between training sessions and one minute rest between repetitions was done. The enzyme-linked immunosorbent assay (ELISA) method for measuring plasma irisin levels and real-time polymerase chain reaction (real-time PCR) method for the relative expression of mRNA of UCP1 and FNDC5 genes were used. Independent t-test was used to compare group differences.Findings: Irisin protein levels after resistance training significantly increased (P < 0.001). Besides, the relative expression of mRNA of FNDC5 (P < 0.001) and UCP1 (P < 0.001) genes significantly increased after exercise.Conclusion: Although research on this theory is a beginning pathway, it can be concluded from the present results that the resistance training through secretion of myokin-like irisin improves body composition possibly through increased energy expenditure in white adipose tissue.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 256 - Serial Number 256
September and October 2013
Pages 1657-1666

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History

  • Receive Date: 08 July 2013
  • Revise Date: 01 June 2024
  • Accept Date: 06 April 2022

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