نوع مقاله : Original Article(s)
نویسندگان
1 استادیار، گروه فارماسیوتیکس، دانشکدهی داروسازی و مرکز تحقیقات سیستمهای نوین دارورسانی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دانشجوی داروسازی، گروه فارماسیوتیکس، دانشکدهی داروسازی و مرکز تحقیقات سیستمهای نوین دارورسانی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 استادیار، گروه بیوتکنولوژی دارویی، دانشکدهی داروسازی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
4 استاد، گروه فارماسیوتیکس، دانشکدهی داروسازی و مرکز تحقیقات سیستمهای نوین دارورسانی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
تازه های تحقیق
سمیه تیموری : PubMed, Google Scholar
ژاله ورشوساز: PubMed, Google Scholar
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: Immunocompromised persons are very susceptible to fungal infections. It is desirable to treat pulmonary fungal infections with specific delivery of drugs to the lungs.
Methods: Zein nanoparticles containing fluconazole were prepared by the anti-solvent method. Their physico-chemical properties were investigated. From the optimal formulation, the inhalation powder was prepared with lactose or mannitol using spray drying, and aerosolization characteristics of the dry powders were evaluated using Anderson's cascade impactor. Antifungal effect was investigated against candida albicans and minimum inhibitory concentration was determined. Cytotoxicity of free fluconazole, a dry powder inhaler containing drug loaded nanoparticles, and dry powder inhaler containing nanoparticles without a drug was also evaluated against the A549 cell line by the MTT method.
Findings: The size of nanoparticles was in the range of 88.70 ± 1.35 to 226.90 ± 5.40 nm, and the percentage of loading efficiency was in the range of 57.58 ± 3.61 to 92.94 ± 0.34. In the prepared dry powder samples, all the powder samples had a high emitted dose percentage. Cytotoxicity test showed that in general none of the groups had significant cytotoxic effects even at the highest studied concentration.
Conclusion: Using lactose as the carrier, can improve the aerodynamic properties of dry powder inhaler compared with mannitol. The prepared inhaled fluconazole powder has a suitable fine particle fraction % and a low median mass aerodynamic diameter and can be well deposited in the lung. In addition, powder containing fluconazole was safe and its effect was comparable with fluconazole.
کلیدواژهها [English]