Protective Effects of Okra Powder (Abelmoschus esculentus) on Histomorphometricl Changes of Testis and Sperm Parameters in High-Fat Diet/Streptozotocin (HFD/STZ) Diabetic Rat

Document Type : Original Article (s)

Authors

1 Professor, Department of Basic Sciences, School of Veterinary Medicine AND Stem Cell and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 PhD Student, Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor, Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Clinical Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Background: Diabetes tends to disorders in male reproductive system. Okra (Abelmoschus esculentus) has antidiabetic and anitoxidant effects. The aim of this study was to investigate the protective effects of okra powder on testis structure and sperm parameters in diabetic rats.Methods: 25 rats were divided randomly into five groups including control, diabetic, diabetic/okra (200 mg/kg), diabetic/metformin (200 mg/kg), and okra (200 mg/kg). Diabetes was induced by high-fat diet fed (HFD), and injection of streptozotocin (STZ) (35 mg/kg) for four weeks. Groups were treated for 4 weeks. After treatment, blood glucose levels, testosterone levels, sperm parameters, and histomorphological structure of testes were studied.Findings: Induction of diabetes decreased testosterone levels, and sperm movement, numbers, and survival. Number of spermatogonia, primary spermatocyte, sertoli, and leydig cells, diameter of seminiferous tubules, and spermiogenesis index decreased significantly in diabetic group as compared to control group (P < 0.05). Okra treatment improved significantly the harmful effects of diabetes on testis tissue and sperm parameters in diabetic rats.Conclusion: The results suggest that okra powder supplemented diet in HFD/STZ diabetic rats improves testosterone level and sperm parameters as well as ameliorates diabetes-induced structural disorders in rat male reproductive system.

Keywords

References

  1. Gheibi S, Bakhtiarzadeh F, Ghasemi A. A review of high fat diet-streptozotocin model for induction of type 2 diabetes in rat. Iran J Endocrinol Metab 2016; 18 (2):135-48. [In Persian].
  2. Pitteloud N, Hardin M, Dwyer AA, Valassi E, Yialamas M, Elahi D, et al. Increasing insulin resistance is associated with a decrease in Leydig cell testosterone secretion in men. J Clin Endocrinol Metab 2005; 90(5): 2636-41.
  3. Ballester J, Munoz MC, Dominguez J, Rigau T, Guinovart JJ, Rodriguez-Gil JE. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl 2004; 25(5): 706-19.
  4. Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res 2008; 40(4): 354-60.
  5. Khaki A, Nouri M, Fatemeh F, Ahmadi-Ashtiani HR, Rastgar H, Rezazadeh S. Protective effects of quercetin on spermatogenesis in streptozotocin induced diabetic rat. Journal of Medicinal Plants 2009: 8(5): 57-64. [In Persian].
  6. Mukesh R, Namita P. Medicinal plants with antidiabetic potential - a review. Am-Euras J Agric Environ Sci 2013; 13(1): 81-94.
  7. Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F. Nutritional quality and health benefits of okra (Abelmoschus esculentus): A review. J Food Process Technol 2015; 6: 458.
  8. Sengkhamparn N, Verhoef R, Schols HA, Sajjaanantakul T, Voragen AG. Characterisation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench). Carbohydr Res 2009; 344(14): 1824-32.
  9. Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K. Investigation of in vivo antioxidant property of Abelmoschus esculentus (L) moench. fruit seed and peel powders in streptozotocin-induced diabetic rats. J Ayurveda Integr Med 2012; 3(4): 188-93.
  10. Kumar DS, Tony DE, Kumar AP, Kumar KA, Rao DBS, Nadendla R. A review on: Abelmoschus esculentus (OKRA). Int Res J Pharm App Sci 2013; 3(4): 129-32.
  11. Kabiri N, Tabandeh MR, Tabatabaie SR. Beneficial effects of pioglitazone and metformin in murine model of polycystic ovaries via improvement of chemerin gene up-regulation. DARU J Pharm Sci 2014; 22: 39.
  12. Binh DV, Dung NTK, Thao LTB, Nhi NB, Chi PV. Macro- and microvascular complications of diabetes induced by high-fat diet and low-dose streptozotocin injection in rats model. J Diabetes Res 2013; 2(3): 50-5.
  13. Parastesh M, Heidarianpour A, Bayat M, Saremi A. Effects of resistance training on serum level of reproductive hormones and sperm parameters in type 2 diabetes rats. J Arak Univ Med Sci 2016; 19(8): 26-36. [In Persian].
  14. Kalantari Hesari A, Shahrooz R, Ahmadi A, Malekinejad H, Saboory E. Crocin prevention of anemia-induced changes in structural and functional parameters of mice testes. J Appl Biomed 2015; 13(3): 213-23.
  15. Skovso S. Modeling type 2 diabetes in rats using high fat diet and streptozotocin. J Diabetes Investig 2014; 5(4): 349-58.
  16. Zakerabasali A, Keshavarz M, Mozafar A, Takhshid M A, Meshkibaf M H. Protective effects of vitamin E and selenium on spermatogenesis in adult male rat insulin-resistant. J Fasa Univ Med Sci 2013; 2(4): 308-13. [In Persian].
  17. Fan S, Zhang Y, Sun Q, Yu L, Li M, Zheng B, et al. Extract of okra lowers blood glucose and serum lipids in high-fat diet-induced obese C57BL/6 mice. J Nutr Biochem 2014; 25(7): 702-9.
  18. Roy A, Shrivastava SL, Mandal SM. Functional properties of Okra Abelmoschus esculentus L. (Moench): Traditional claims and scientific evidences. Plant Science Today 2014; 1(3): 121-30.
  19. Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005; 52(4): 313-20.
  20. Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Rashidi Nooshabadi MR. Effects of hydro-alcoholic extract of Rhus coriaria (Sumac) seeds on reproductive complications of nicotinamide-streptozotocin induced type-2 diabetes in male mice. World J Mens Health 2014; 32(3): 151-8.
  21. Jarret RL, Wang ML, Levy IJ. Seed oil and fatty acid content in okra (Abelmoschus esculentus) and related species. J Agric Food Chem 2011; 59(8): 4019-24.
  22. Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozocin-induced diabetic rats. Comp Biochem Physiol C Toxicol Pharmacol 2003; 135C(3): 357-64.
  23. La Vignera S, Condorelli R, Vicari E, D'Agata R, Calogero AE. Diabetes mellitus and sperm parameters. J Androl 2012; 33(2): 145-53.
  24. Hadi MA, Zaidan HK, Natah TM, Al-Saadi AH. Protective effect of plants extracts mixture on sperm abnormalities, testicular and epididymal tissues in diabetic male rats. Journal of Natural Sciences Research 2013; 3(9): 28-37.
  25. Liu GL, Zhang YM, Dai DZ, Ding MJ, Cong XD, Dai Y. Male hypogonadism induced by high fat diet and low dose streptozotocin is mediated by activated endoplasmic reticulum stress and IkappaBbeta and attenuated by argirein and valsartan. Eur J Pharmacol 2013; 713(1-3): 78-88.
  26. Cheung KK, Luk AO, So WY, Ma RC, Kong AP, Chow FC, et al. Testosterone level in men with type 2 diabetes mellitus and related metabolic effects: A review of current evidence. J Diabetes Investig 2015; 6(2): 112-23.
  27. Turk G, Sonmez M, Aydin M, Yuce A, Gur S, Yuksel M, et al. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr 2008; 27(2): 289-96.
  28. Mokhtari M, Shariatie M, Gharamanie R. Effect of Trigonella foenum - graecum L. seed extract on concentration of testosterone and spermatogenesis in rats. Journal of Medicinal Plants 2008; 7(1): 12-20. [In Persian].
  29. Shariati M, Sharifi E, Kaveh M. The effect of phoenix dactylifera (Date-palm) pit powder on testosterone level and germ cells in adult male rats. J Zanjan Univ Med Sci 2007; 15(61): 21-8. [In Persian].
Journal of Isfahan Medical School
Volume 35, Issue 438
May and June 2017
Pages 834-841

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History

  • Receive Date: 07 May 2017
  • Revise Date:
  • Accept Date: 06 April 2022

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