The Histological Study of the Interactions between Rabbit Decellularized Esophagus Scaffold and the Blastema Tissue Obtained from the Pinna of New Zealand White Rabbit

Document Type : Original Article (s)

Authors

1 Professor, Department of Biology, School of Science AND Cell and Molecular Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran

3 Associate Professor, Department of Biology, School of Science AND Cell and Molecular Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

4 Associate Professor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran

5 Assistant Professor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Background: The extracellular matrix (ECM) is a key component during regeneration and maintenance of tissues and organs and has inductive effects on the behaviors of cells. The objective of this study was to investigate the interactions between rabbit's esophageal scaffold with blastema tissue cells obtained from the pinnas of New Zealand white rabbits. Blastema is a collection of undifferentiated cells that are able to propagate and differentiate in a manner similar to embryonic cells.Methods: In this experimental study, after removing the esophagus, physical and chemical decellularization methods including snap-freeze thaw and treatment with Triton X-100 and sodium dodecyl sulfate (SDS), as detergents, were performed. To prepare the blastema tissue, the pinnas of New Zealand white rabbits were punched and after 48 hours, the blastema tissue was obtained by a second punch. After washing, the scaffolds were assembled inside the blastema rings and cultured in Dulbecco's Modified Eagle's Medium (DMEM).Findings: Histological studies demonstrated that using snap-freeze thaw and then treatment with 1% Triton X-100 for 24 hours followed by 0.5% SDS for 48 hours was the best decellularization procedure for preparing scaffolds from rabbit's esophagus. Furthermore, adhesion and migration of blastema tissue cells to esophageal scaffold were observed after two weeks of culture.Conclusion: It can be concluded that the extracellular matrix of the decellularized esophagus can have inductive effects on the proliferation, migration and adhesion of the blastema tissue cells.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 261 - Serial Number 261
September and October 2014
Pages 1865-1875

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History

  • Receive Date: 26 June 2013
  • Revise Date: 26 May 2024
  • Accept Date: 06 April 2022

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