تأثیر یک دوره تمرین ورزشی مقاومتی با شدت بالا بر سطوح اینترلوکین- 10، Tumor necrosis factor-alpha (TNF-α) و Brain-derived neurotrophic factor (BDNF) در مغز رت‌های Lewis مبتلا به آنسفالومیلیت خود ایمن تجربی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

3 استادیار، گروه بافت‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی بابل، بابل، ایران

چکیده

مقدمه: Multiple sclerosis (MS)، بیماری التهابی دمیلینه کننده‌ی مزمن Central nervous system (CNS) است. واکنش التهابی با تنظیم افزایشی انواعی از سیتوکاین‌ها در ضایعات MS همبستگی دارد. هدف از انجام این تحقیق، بررسی تأثیر برنامه‌ی ورزشی مقاومتی با شدت بالا در پیش‌گیری از بیماری آنسفالومیلیت خود ایمنی (Experimental autoimmune encephalomyelitis یا EAE) (مدلی برای مطالعه‌ی MS) بود.روش‌ها: تمرینات مقاومتی به مدت 6 هفته و 5 روز در هفته انجام شد. شروع برنامه‌ی تمرینی با بار معادل ۵۰ درصد وزن بدن رت بود. حیوانات در دو جلسه‌ی اول، 10-۸ تکرار (بالا رفتن از نردبان) را با فواصل استراحت ۲ دقیقه‌ای انجام دادند. رت‌ها با نخاع خوکچه‌ی هندی و Complete Freund's adjuvant ایمونیزه شدند. روند بیماری و تغییرات وزن رت‌ها روزانه مورد ارزیابی قرار گرفت. مدل آنسفالومیلیت خود ایمنی در انتهای هفته‌ی ششم تمرینات القا شد.یافته‌ها: تمرین مقاومتی در رت‌های ماده‌ی نژاد Lewis مبتلا به آنسفالومیلیت خود ایمنی، منجر به کاهش معنی‌دار Tumor necrosis factor alpha (TNF-α) (001/0 = P) و افزایش معنی‌دار اینترلوکین- 10 (Interleukin-10 یا IL-10) (001/0 = P) شد، اما تغییر معنی‌داری در عامل مشتق شده از مغز (Brain-derived neurotrophic factor یا BDNF) ایجاد نکرد (055/0 = P). همچنین، علایم بالینی در رت‌های تمرین کرده با تأخیر ظاهر شد.نتیجه‌گیری: با توجه به نتایج، به نظر می‌رسد تمرینات مقاومتی با مدت و شدت لازم موجب تأخیر در بروز و کاهش شدت علایم بالینی، عدم تغییر BDNF، کاهش TNF-α و افزایش IL-10 در رت‌های Lewis مبتلا به آنسفالومیلیت خود ایمنی می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of High-Intensity Resistance Exercise Period on the Levels of Interleukin-10 (IL-10), Tumor Necrosis Factor-Alpha (TNF-α), and Brain-Derived Neurotrophic Factor (BDNF) in the Brain of Lewis Rats with Experimental Autoimmune Encephalomyelitis

نویسندگان [English]

  • Seyed Mojtaba Hosseini 1
  • Zia Fallah-Mohammadi 2
  • Farideh Feizi 3
1 PhD Student, Department of Exercise Physiology, School of Sport Sciences, University of Mazandaran, Babolsar, Iran
2 Associate Professor, Department of Exercise Physiology, School of Sport Sciences, University of Mazandaran, Babolsar, Iran
3 Assistant Professor, Department of Histology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
چکیده [English]

Background: Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is a correlation between up-regulation of a variety of cytokines with inflammatory reactions in multiple sclerosis lesions. This study aimed to investigate the effects of high-intensity resistance exercise program on prevention of experimental autoimmune encephalomyelitis (EAE) (a model for studying multiple sclerosis).Methods: Resistance exercises were performed for 6 weeks, 5 days a week. Training program started with 50% of rats' body weight. In the first session, animals performed 8 to 10 repetitions (up the ladder) with 2-minute rest intervals. Rats were immunized with spinal cord and complete adjuvant of guinea pigs. Disease process and changes in rats' weights were measured every day. Experimental autoimmune encephalomyelitis model was induced at the end of the sixth week of training.Findings: Resistance training in Lewis female rats with experimental autoimmune encephalomyelitis resulted in a significant reduction in tumor necrosis factor-alpha (TNF-α) (P = 0.001) and a significant increase in interleukin-10 (IL-10) (P = 0.001). However, it did not cause significant changes in Brain-Derived Neurotrophic Factor (BDNF) (P = 0.055). In addition, clinical symptoms in exercised rats were postponed.Conclusion: Regarding the results, it appears that resistance exercises with necessary duration and intensity delay the onset, reduce the severity of clinical symptoms, and decrease TNF-α, increase IL-10, with no change in BDNF, in Lewis rats with experimental autoimmune encephalomyelitis.

کلیدواژه‌ها [English]

  • Multiple Sclerosis
  • Cytokines
  • Resistance Training
  • Experimental autoimmune encephalomyelitis

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مجله دانشکده پزشکی اصفهان
دوره 35، شماره 422 - شماره پیاپی 422
فروردین و اردیبهشت 1396
صفحه 270-277

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سابقه مقاله

  • تاریخ دریافت: 28 آبان 1395
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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