Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 29 155 2011 10 23 A Study of CD4+Foxp3+Treg and CD8+Foxp3+Treg Cells in Patients with Rheumatoid Arthritis A Study of CD4+Foxp3+Treg and CD8+Foxp3+Treg Cells in Patients with Rheumatoid Arthritis 1412 1419 13530 FA Mahtab Tapak Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Alireza Andalib Associate Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-2032-6281 Peyman Mottaghi Associate Professor, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Shady Babazadeh Department of Radiotherapy and Oncology, Seyed-Al-Shohada Hospital, Isfahan University of Medical Sciences, Isfahan, Iran Abbas Rezaei Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mansoor Salesi Assistant Professor, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-7108-6188 Journal Article 2011 08 28 Background: Rheumatoid arthritis (RA) is defines as a Th1 dominant disease. Tregs cell are a rather new group of T cells that regulates other immune cells including Th1 and Th2. Foxp3 is a lineage-determining factor for Treg cells. Several subsets of Foxp3+regulatory T cells are ever identified. In this study we investigated the frequency of CD4+Foxp3+Treg and CD8+Foxp3+Treg cells in patients with rheumatoid arthritis.Methods: Peripheral blood samples were obtained from 31 patients with rheumatoid arthritis and 21 healthy controls. Monoclonal antibodies including anti-CD4 and anti-CD8 and anti-Foxp3 were used and the staining process was performed. Flow cytometry were applied to evaluate the markers.Findings: The percentage of CD4+Foxp3+Treg cells was 1.03% ± 0.28 % in rheumatoid arthritis and 1.25% ± 0.3% in control group (P = 0.010). The percentage of CD8+Foxp3+ Treg cells was 0.79 ± 0.18, and 0.63 ± 0.16 in rheumatoid arthritis and control groups respectively (P = 0.002). The WBC and Lymphocytes population in rheumatoid arthritis group were higher than control group (P = 0.001). Conclusion: These data demonstrate that frequency of Treg cells might be involved in the pathogenesis of rheumatoid arthritis. This may be a contributory factor in the susceptibility to rheumatoid arthritis (Th1 dominant), or it may achieved during the progression of the disease. Background: Rheumatoid arthritis (RA) is defines as a Th1 dominant disease. Tregs cell are a rather new group of T cells that regulates other immune cells including Th1 and Th2. Foxp3 is a lineage-determining factor for Treg cells. Several subsets of Foxp3+regulatory T cells are ever identified. In this study we investigated the frequency of CD4+Foxp3+Treg and CD8+Foxp3+Treg cells in patients with rheumatoid arthritis.Methods: Peripheral blood samples were obtained from 31 patients with rheumatoid arthritis and 21 healthy controls. Monoclonal antibodies including anti-CD4 and anti-CD8 and anti-Foxp3 were used and the staining process was performed. Flow cytometry were applied to evaluate the markers.Findings: The percentage of CD4+Foxp3+Treg cells was 1.03% ± 0.28 % in rheumatoid arthritis and 1.25% ± 0.3% in control group (P = 0.010). The percentage of CD8+Foxp3+ Treg cells was 0.79 ± 0.18, and 0.63 ± 0.16 in rheumatoid arthritis and control groups respectively (P = 0.002). The WBC and Lymphocytes population in rheumatoid arthritis group were higher than control group (P = 0.001). Conclusion: These data demonstrate that frequency of Treg cells might be involved in the pathogenesis of rheumatoid arthritis. This may be a contributory factor in the susceptibility to rheumatoid arthritis (Th1 dominant), or it may achieved during the progression of the disease. https://jims.mui.ac.ir/article_13530_e96560b68b0d11e80e48fb30b5f8b01d.pdf