Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 30 205 2012 10 22 The Association between Gelatinase B and Lung Cancer in Smokers and Non-Smokers The Association between Gelatinase B and Lung Cancer in Smokers and Non-Smokers 13882 FA Majid Motovali-Bashi Assistant Professor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran 0000-0003-4380-0166 Simin Hemmati Assistant Professor, Department of Oncology and Radiotherapy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-7517-8987 Somayeh Taghvaei MSc Student, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran Journal Article 2011 11 29 Background: Gelatinase B is not only involved in metastasis, but also alters and processes growth factors, growth factor receptors, angiogenic factors, and other proteinases and thus affects early carcinogenesis. In other words, it has a fundamental role in initiation and development of cancer. Genetic polymorphism in the promoter of gelatinase B has been reported to be associated with the risk of several cancers including lung cancer. Methods: Genotyping of gelatinase B was carried out by taking blood samples from 172 patients with lung cancer and 100 controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. The observed numbers of each gelatinase B genotype were compared with that expected for a population in Hardy-Weinberg equilibrium by χ2 test. The significance of the differences of the observed alleles and genotypes between groups was tested using the odds ratio (OR) analysis. Findings: The percentage of smokers in patients was more than controls (57.5% vs. 30%). Distribution of gelatinase B genotype was significantly associated with the risk of lung cancer [OR = 2.56; 95% confidence interval (CI) = 0.06-23.82]. Conclusion: Our results indicated that smokers who carry TT and CT+TT genotypes have 4 (OR = 3.45; 95% CI = 1.28-9.24) and 15 (OR = 14.66; 95% CI = 3.95-53.47) fold risks of lung cancer, respectively. Keywords: Gelatinase B, Restriction fragment length polymorphism, Polymerase chain reaction, Genotyping, Smokers, Lung cancer Background: Gelatinase B is not only involved in metastasis, but also alters and processes growth factors, growth factor receptors, angiogenic factors, and other proteinases and thus affects early carcinogenesis. In other words, it has a fundamental role in initiation and development of cancer. Genetic polymorphism in the promoter of gelatinase B has been reported to be associated with the risk of several cancers including lung cancer. Methods: Genotyping of gelatinase B was carried out by taking blood samples from 172 patients with lung cancer and 100 controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. The observed numbers of each gelatinase B genotype were compared with that expected for a population in Hardy-Weinberg equilibrium by χ2 test. The significance of the differences of the observed alleles and genotypes between groups was tested using the odds ratio (OR) analysis. Findings: The percentage of smokers in patients was more than controls (57.5% vs. 30%). Distribution of gelatinase B genotype was significantly associated with the risk of lung cancer [OR = 2.56; 95% confidence interval (CI) = 0.06-23.82]. Conclusion: Our results indicated that smokers who carry TT and CT+TT genotypes have 4 (OR = 3.45; 95% CI = 1.28-9.24) and 15 (OR = 14.66; 95% CI = 3.95-53.47) fold risks of lung cancer, respectively. Keywords: Gelatinase B, Restriction fragment length polymorphism, Polymerase chain reaction, Genotyping, Smokers, Lung cancer https://jims.mui.ac.ir/article_13882_b7cda51a7b31b77fe2d5c1ee19f33496.pdf