Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 30 209 2012 11 21 Angiotensin Converting Enzyme Insertion/Deletion Polymorphism and Occlusion of Vein Grafts in Long-term Post-CABG Angiotensin Converting Enzyme Insertion/Deletion Polymorphism and Occlusion of Vein Grafts in Long-term Post-CABG 1622 1630 13906 FA Neda Zeinali Student of Pharmacy, Students' Research Committee, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Mohammad Hashemi Professor, Department of Cardiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mohsen Mirmohammad Sadeghi Assistant Professor, Department of Cardiothoracic Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Hamid Mirmohammad Sadeghi Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Ali Mohammad Sabzghabaee Associate Professor, Isfahan Clinical Toxicology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-8115-4615 Journal Article 2012 10 11 Background: The relation between angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism and cardiovascular diseases was reported previously but the role of this polymorphism and the occlusion of vein grafts in long-term post coronary artery bypass graft (CABG) surgery still has remained controversial. The aim of the present study was to investigate any probable relationship between ACE I/D polymorphism and the atherosclerotic occlusion of vein grafts. Methods: Patients who undergone CABG surgery more than five years ago, participated in this cross-sectional study. Occlusion of vein graft was determined by angiography. The ACE I/D polymorphism was detected by polymerase chain reaction (PCR) based restriction analysis. Findings: A total of 102 patients (84 males) were enrolled to the study. The frequency distribution of DD, ID, and II polymorphisms were 23.6%, 62.7%, and 13.7%, respectively. There were no differences among genotypic groups regarding the number of occluded, diseased, and atherosclerosis-free vein grafts (P = 0.6, 0.7, and 0.18, respectively). Patients with II genotype had the same number of completely occluded vein grafts in a marginally significant shorter time after CABG (P = 0.06) compared with the other groups. Conclusion: Although the results of our study indicated no association of ACE I/D polymorphisms and the occlusion of vein grafts long-term post-CABG, ACE II genotype may accelerate the rate of occlusion in vein grafts. Keywords: INDEL mutation, Angiotensin converting enzyme, Coronary artery bypass, Vascular graft occlusion Background: The relation between angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism and cardiovascular diseases was reported previously but the role of this polymorphism and the occlusion of vein grafts in long-term post coronary artery bypass graft (CABG) surgery still has remained controversial. The aim of the present study was to investigate any probable relationship between ACE I/D polymorphism and the atherosclerotic occlusion of vein grafts. Methods: Patients who undergone CABG surgery more than five years ago, participated in this cross-sectional study. Occlusion of vein graft was determined by angiography. The ACE I/D polymorphism was detected by polymerase chain reaction (PCR) based restriction analysis. Findings: A total of 102 patients (84 males) were enrolled to the study. The frequency distribution of DD, ID, and II polymorphisms were 23.6%, 62.7%, and 13.7%, respectively. There were no differences among genotypic groups regarding the number of occluded, diseased, and atherosclerosis-free vein grafts (P = 0.6, 0.7, and 0.18, respectively). Patients with II genotype had the same number of completely occluded vein grafts in a marginally significant shorter time after CABG (P = 0.06) compared with the other groups. Conclusion: Although the results of our study indicated no association of ACE I/D polymorphisms and the occlusion of vein grafts long-term post-CABG, ACE II genotype may accelerate the rate of occlusion in vein grafts. Keywords: INDEL mutation, Angiotensin converting enzyme, Coronary artery bypass, Vascular graft occlusion https://jims.mui.ac.ir/article_13906_19df34e5a4cbdb9c7a41acd3fbd71222.pdf