Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 31 250 2013 09 23 Prevalence of Rotavirus Gastroenteritis and Circulating Genotypes in Children with Acute Gastroenteritis Admitted in Imam Hossein Hospital, Isfahan, Iran; 2013 Prevalence of Rotavirus Gastroenteritis and Circulating Genotypes in Children with Acute Gastroenteritis Admitted in Imam Hossein Hospital, Isfahan, Iran; 2013 1360 1368 14141 FA Sharareh Moghim Assistant Professor, Nosocomial Infection Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-7134-3145 Hossein Fazeli Assistant Professor, Infectious Diseases and Tropical Medicine Research Center AND Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran Bahram Nasr Esfahani Associate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0003-3264-3361 Hoda Jafari-Modrek Student of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article 2013 08 04 Background: Human Rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. In recent years, rotavirus genotyping by reverse transcription polymerase chain reaction (RT-PCR) has provided valuable information about the diversity of rotaviruses circulating worldwide. The purpose of the present study was to monitor the prevalence of the different circulating G and P genotypes of rotaviruses in Isfahan, Iran.Methods: During the period of spring 2013, a total of 117 stool samples were collected from children less than 3 years old who were hospitalized for acute gastroenteritis in Imam Hossein Hospital, Isfahan, Iran, as the referral pediatrics hospital. Rotavirus-associated diarrhea was investigated in fecal specimens with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Rotavirus-positive specimens were typed by the nested reverse transcription (RT) polymerase chain reaction (Nested RT-PCR) using different types of specific primers.Findings: Out of the 117 collected samples, 35.3% (41 cases) tested positive for rotavirus. The frequency of G1P8, G8P4, G8P8, G1P4 and G1G4P8 types was 65.9%, 17.1%, 12.2%, 2.4% and 2.4%, respectively.Conclusion: In this study, the prevalence rotavirus infection among children less than 3 years of age in Isfahan was determined. The dominant genotyped was G1P8. Other studies in important areas of the country are needed to decide about future vaccine formulation in Iran.  Background: Human Rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. In recent years, rotavirus genotyping by reverse transcription polymerase chain reaction (RT-PCR) has provided valuable information about the diversity of rotaviruses circulating worldwide. The purpose of the present study was to monitor the prevalence of the different circulating G and P genotypes of rotaviruses in Isfahan, Iran.Methods: During the period of spring 2013, a total of 117 stool samples were collected from children less than 3 years old who were hospitalized for acute gastroenteritis in Imam Hossein Hospital, Isfahan, Iran, as the referral pediatrics hospital. Rotavirus-associated diarrhea was investigated in fecal specimens with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Rotavirus-positive specimens were typed by the nested reverse transcription (RT) polymerase chain reaction (Nested RT-PCR) using different types of specific primers.Findings: Out of the 117 collected samples, 35.3% (41 cases) tested positive for rotavirus. The frequency of G1P8, G8P4, G8P8, G1P4 and G1G4P8 types was 65.9%, 17.1%, 12.2%, 2.4% and 2.4%, respectively.Conclusion: In this study, the prevalence rotavirus infection among children less than 3 years of age in Isfahan was determined. The dominant genotyped was G1P8. Other studies in important areas of the country are needed to decide about future vaccine formulation in Iran.  https://jims.mui.ac.ir/article_14141_ca6d6b468064abd249fb3533d4ddaed7.pdf