Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 32 298 2014 09 23 The Effect of Extremely Low-Frequency Magnetic Fields on the Level of Serotonin Metabolite in the Raphe Nuclei of Adult Male Rat The Effect of Extremely Low-Frequency Magnetic Fields on the Level of Serotonin Metabolite in the Raphe Nuclei of Adult Male Rat 1354 1362 14416 FA Daryoush Shahbazi Professor, Department of Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-8538-1898 Leila Shiri MSc Student, Department of Biomedical Engineering, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran Hojatollah Alaei Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-9474-5756 Naser Naghdi Professor, Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Saeid Kermani Assistant Professor, Department of Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Hossein Afrouzi PhD Student, Department of Chemistry, School of Basic Sciences, Lorestan University, Khorramabad, Iran Ali Kiani PhD Student, Department of Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mojtaba Akbari PhD Student, Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran 0000-0002-5777-9612 Journal Article 2013 11 30 Background: According to several studies, the extremely low-frequency magnetic fields (ELF fields) affect extensively on biological system. These fields can influence the nervous and neurotransmitter system due to the electrical nature of them. The influences in some cases are therapeutic and sometimes are destructive. The purpose of this study was to investigate the effect of extremely low-frequency magnetic fields, with frequency of 10 Hz and intensity of 720 to 540 microtesla, on the level of serotonin metabolite, 5-Hydroxyindoleacetic Acid (5-HIAA), in the raphe nuclei of adult male rat.Methods: Using a magnetic coil, the extremely low-frequency magnetic field, with 690 microtesla strength at the center of the coil, was produced. 6 rats were under this field 3 hours daily for 15 consecutive days. Then, 6 samples were collected from the raphe nucleus of each rat using microdialysis technique. Each sample was 40 microliters in volume. Then, the serotonin metabolite levels in each sample were measured via high-pressure liquid chromatography and compared with its control sample.Findings: Extremely low-frequency magnetic field with mentioned characteristics significantly decreased the level of serotonin metabolite in the raphe nucleus compared to the control group (P < 0.05).Conclusion: It can be concluded that extremely low-frequency magnetic fields affect the serotonergic system and can be used to treat some diseases. Background: According to several studies, the extremely low-frequency magnetic fields (ELF fields) affect extensively on biological system. These fields can influence the nervous and neurotransmitter system due to the electrical nature of them. The influences in some cases are therapeutic and sometimes are destructive. The purpose of this study was to investigate the effect of extremely low-frequency magnetic fields, with frequency of 10 Hz and intensity of 720 to 540 microtesla, on the level of serotonin metabolite, 5-Hydroxyindoleacetic Acid (5-HIAA), in the raphe nuclei of adult male rat.Methods: Using a magnetic coil, the extremely low-frequency magnetic field, with 690 microtesla strength at the center of the coil, was produced. 6 rats were under this field 3 hours daily for 15 consecutive days. Then, 6 samples were collected from the raphe nucleus of each rat using microdialysis technique. Each sample was 40 microliters in volume. Then, the serotonin metabolite levels in each sample were measured via high-pressure liquid chromatography and compared with its control sample.Findings: Extremely low-frequency magnetic field with mentioned characteristics significantly decreased the level of serotonin metabolite in the raphe nucleus compared to the control group (P < 0.05).Conclusion: It can be concluded that extremely low-frequency magnetic fields affect the serotonergic system and can be used to treat some diseases. https://jims.mui.ac.ir/article_14416_547dcee2f9ccacd21cb7a6a758ca8697.pdf