Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 33 328 2015 04 21 Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line 400 409 14598 FA Maryam Khorramizadeh Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Seyed Ali Hosein Saberi Associate Professor, Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Parvaneh Shokrani Professor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mohammad Javad Tahmasebi-Birgani Professor, Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Seyed Hosein Hejazi Professor, Skin Disease and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Ali Reza Amouhedari Radiation Oncologist, Milad Hospital, Isfahan, Iran Journal Article 2015 01 12 Background: Renal cell carcinoma is considered to be a radioresistant tumor. Consequently, radiotherapy is used mainly for palliation of metastases or local tumor growth. The aim of this study was to evaluate the efficacy of the radiation protocols with small fractions on cell killing of ACHN human renal cell carcinoma cell line.Methods: After cell culture, the ACHN cells were exposed to 6, 8 and 10 Gy of single fraction and 3 × 2, 4 × 2 and 5 × 2 Gy of two fractions. Viability was calculated using MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium].Findings: Exposing cultured cells to small fractions of radiation resulted to a mean decrease of 70% in cell survival over 120 hours after radiation. Survival fraction reduced with increasing the dose per fraction. Two fractions 5 × 2 Gy showed significant effect on cell survival compared to single fraction 10 Gy (P = 0.02)Conclusion: Our results indicate that the small fraction is a valuable alternative to provide local tumor control. Background: Renal cell carcinoma is considered to be a radioresistant tumor. Consequently, radiotherapy is used mainly for palliation of metastases or local tumor growth. The aim of this study was to evaluate the efficacy of the radiation protocols with small fractions on cell killing of ACHN human renal cell carcinoma cell line.Methods: After cell culture, the ACHN cells were exposed to 6, 8 and 10 Gy of single fraction and 3 × 2, 4 × 2 and 5 × 2 Gy of two fractions. Viability was calculated using MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium].Findings: Exposing cultured cells to small fractions of radiation resulted to a mean decrease of 70% in cell survival over 120 hours after radiation. Survival fraction reduced with increasing the dose per fraction. Two fractions 5 × 2 Gy showed significant effect on cell survival compared to single fraction 10 Gy (P = 0.02)Conclusion: Our results indicate that the small fraction is a valuable alternative to provide local tumor control. https://jims.mui.ac.ir/article_14598_11add9933745e1c3ee82d10f03d853b5.pdf