Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 34 415 2017 02 19 Expression of Epstein-Barr Virus Nuclear Antigen (EBNA) in Papillary Thyroid Carcinoma in a Population of Patients in Isfahan City, Iran Expression of Epstein-Barr Virus Nuclear Antigen (EBNA) in Papillary Thyroid Carcinoma in a Population of Patients in Isfahan City, Iran 1648 1652 15141 FA Mozhgan Mokhtari Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0003-1645-945X Mona Bahreini-Isfahani General Practitioner, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Faegheh Naji General Practitioner, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Ali Safaei Student of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-8718-6650 Journal Article 2016 07 17 Background: Papillary thyroid carcinoma is the most prevalent malignancy of thyroid gland and is significantly lymphtropic. Epstein-Barr virus (EBV) infection may play an important role in the development of this malignancy. The aim of this study was to investigate the expression of Epstein-Barr virus nuclear antigen (EBNA), as a probable risk factor, in papillary thyroid carcinoma in a population of patients in Isfahan city, Iran, in the year 2015.Methods: This study was performed on 43 cases of papillary thyroid carcinoma confirmed by pathologist. Using qualitative polymerase chain reaction (PCR) method in formalin-fixed paraffin-embedded tissue specimens, EBNA expression in tumor tissue was investigated. Information on age, sex, tumor size, lymph node status and metastasis were collected from the patients' medical records. The collected data were analyzed using independent t and chi-square tests via SPSS software.Findings: EBNA antigen expression was detected in 9 cases (20.9%). The relationship between different factors and EBNA antigen expression showed a significant relationship between patients' age and gene expression (P < 0.05). In contrast, other factors were not significantly correlated with EBNA gene expression (P > 0.05).Conclusion: According to the results of this study, Epstein-Barr virus may have a role in pathogenesis of papillary thyroid carcinoma. Further studies with control group are necessary to confirm the issue. Background: Papillary thyroid carcinoma is the most prevalent malignancy of thyroid gland and is significantly lymphtropic. Epstein-Barr virus (EBV) infection may play an important role in the development of this malignancy. The aim of this study was to investigate the expression of Epstein-Barr virus nuclear antigen (EBNA), as a probable risk factor, in papillary thyroid carcinoma in a population of patients in Isfahan city, Iran, in the year 2015.Methods: This study was performed on 43 cases of papillary thyroid carcinoma confirmed by pathologist. Using qualitative polymerase chain reaction (PCR) method in formalin-fixed paraffin-embedded tissue specimens, EBNA expression in tumor tissue was investigated. Information on age, sex, tumor size, lymph node status and metastasis were collected from the patients' medical records. The collected data were analyzed using independent t and chi-square tests via SPSS software.Findings: EBNA antigen expression was detected in 9 cases (20.9%). The relationship between different factors and EBNA antigen expression showed a significant relationship between patients' age and gene expression (P < 0.05). In contrast, other factors were not significantly correlated with EBNA gene expression (P > 0.05).Conclusion: According to the results of this study, Epstein-Barr virus may have a role in pathogenesis of papillary thyroid carcinoma. Further studies with control group are necessary to confirm the issue. https://jims.mui.ac.ir/article_15141_124243dcc405103dcf9419f38c89f078.pdf