Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 35 454 2017 12 22 The Effect of Erythropoietin on Neuronal Apoptosis in Hippocampal Dentate Gyrus in Streptozotocin-Induced Alzheimer's Disease in Rats The Effect of Erythropoietin on Neuronal Apoptosis in Hippocampal Dentate Gyrus in Streptozotocin-Induced Alzheimer's Disease in Rats 1593 1598 15415 10.22122/jims.v35i454.8745 FA Moloud Shabrang Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Bahman Rashidi Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0003-1133-9571 Hojjatallah Alaei Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-9474-5756 Mohammad Reza Sharifi Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-1538-9034 Parham Reisi Associate Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article 2017 09 02 Background: Alzheimer's disease is a disorder associated with neuronal degeneration in the areas involve in learning and memory. It has been proposed that erythropoietin probably has neuroprotective effects. Therefore, the aim of the present study was to evaluate the effect of erythropoietin on neuronal apoptosis in the granular layer of dentate gyrus of the hippocampus in an animal model of Alzheimer's disease.Methods: Animal model of Alzheimer's was established via bilateral intracerebroventricular (ICV) injection of streptozotocin (STZ). After 2 weeks and observation of cognitive disorder, rats received intraperitoneal (IP) injection of erythropoietin every other day with a dose of 5000 IU/kg for 2 weeks. Finally, the rats were anesthetized and their brains were dissected for immunohistochemical study. Terminal deoxynucleotidyl transferase biotin-dUTP Nick-End Labeling (TUNEL) method was used to study neuronal apoptosis.Findings: Intracerebroventricular injection of streptozotocin increased the number of apoptotic cells in the granular layer of dentate gyrus significantly (P < 0.05). Although erythropoietin had no effect on the control rats, it significantly reduced the number of apoptotic cells in the lesion group (P < 0.01)Conclusion: The results of this study indicate that erythropoietin may be effective in reducing the complications of neurodegenerative diseases by reducing neuronal apoptosis. Background: Alzheimer's disease is a disorder associated with neuronal degeneration in the areas involve in learning and memory. It has been proposed that erythropoietin probably has neuroprotective effects. Therefore, the aim of the present study was to evaluate the effect of erythropoietin on neuronal apoptosis in the granular layer of dentate gyrus of the hippocampus in an animal model of Alzheimer's disease.Methods: Animal model of Alzheimer's was established via bilateral intracerebroventricular (ICV) injection of streptozotocin (STZ). After 2 weeks and observation of cognitive disorder, rats received intraperitoneal (IP) injection of erythropoietin every other day with a dose of 5000 IU/kg for 2 weeks. Finally, the rats were anesthetized and their brains were dissected for immunohistochemical study. Terminal deoxynucleotidyl transferase biotin-dUTP Nick-End Labeling (TUNEL) method was used to study neuronal apoptosis.Findings: Intracerebroventricular injection of streptozotocin increased the number of apoptotic cells in the granular layer of dentate gyrus significantly (P < 0.05). Although erythropoietin had no effect on the control rats, it significantly reduced the number of apoptotic cells in the lesion group (P < 0.01)Conclusion: The results of this study indicate that erythropoietin may be effective in reducing the complications of neurodegenerative diseases by reducing neuronal apoptosis. https://jims.mui.ac.ir/article_15415_a59a9c2e2ec7250a16e4fff6367e953e.pdf