Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 36 479 2018 06 22 The Effect of Ultrasound Parameters on the Destruction of 4T1 Cells in the Presence of Gold Nanoparticles The Effect of Ultrasound Parameters on the Destruction of 4T1 Cells in the Presence of Gold Nanoparticles 524 529 15569 10.22122/jims.v36i479.10334 FA Ahmad Shanei Associate Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-5999-0932 Mohammad Reza Salamat Associate Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-8994-2541 Hossein Hejazi Associate Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Marziyeh Mirzaeiyan MSc Student, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article 2018 06 23 Background: The ultrasound parameters such as intensity and time of radiation play an important role in cellular degradation. In this study, the effect of ultrasound radiation parameters on the degradation of 4T1 cells in the presence of gold nanoparticles was investigated.Methods: The 4T1 cells were cultured. The cells were divided into 4 groups including control, gold nanoparticle, ultrasound alone, and ultrasound at the presence of gold nanoparticle. Ultrasound irradiation were performed on the cells at intensity of 0.5, 1.0, and 1.5 W/cm2, for 1, 1.5, and 2 minutes. The viability of cells was determined using MTT assay.Findings: There was a significant difference in cell viability between the ultrasound and gold nanoparticle group and the other groups at intensity of 1.0 and 1.5 W/cm2. Moreover, there was a significant difference between the ultrasound alone and the control groups at intensity of 1.0 and 1.5 W/cm2.Conclusion: Using ultrasound in the presence of gold nanoparticles increase cell death via increasing nuclei cavitation phenomenon. Background: The ultrasound parameters such as intensity and time of radiation play an important role in cellular degradation. In this study, the effect of ultrasound radiation parameters on the degradation of 4T1 cells in the presence of gold nanoparticles was investigated.Methods: The 4T1 cells were cultured. The cells were divided into 4 groups including control, gold nanoparticle, ultrasound alone, and ultrasound at the presence of gold nanoparticle. Ultrasound irradiation were performed on the cells at intensity of 0.5, 1.0, and 1.5 W/cm2, for 1, 1.5, and 2 minutes. The viability of cells was determined using MTT assay.Findings: There was a significant difference in cell viability between the ultrasound and gold nanoparticle group and the other groups at intensity of 1.0 and 1.5 W/cm2. Moreover, there was a significant difference between the ultrasound alone and the control groups at intensity of 1.0 and 1.5 W/cm2.Conclusion: Using ultrasound in the presence of gold nanoparticles increase cell death via increasing nuclei cavitation phenomenon. https://jims.mui.ac.ir/article_15569_77bd061c0e645ca42b087a6d0d06c019.pdf