Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 37 557 2020 02 20 Green Synthesis of Silver Nanoparticles Using Crocus Sativus (Saffron) in Various Physical Conditions Green Synthesis of Silver Nanoparticles Using Crocus Sativus (Saffron) in Various Physical Conditions 1381 1387 16000 10.22122/jims.v37i557.12114 FA Saeedeh Askarian Assistant Professor, Department of Medical Biotechnology, School of Paramedical Sciences AND Neuroscience Research Center, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran 0000-0001-7092-5947 Leila Sadat Seyedabadi Student, Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran 0000-0002-1484-7005 Reza Kazemi-Oskuee Associate Professor, Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 0000-0001-9166-2939 Majid Darroudi Associate Professor, Nuclear Medicine Research Center (NMRC), Mashhad University of Medical Sciences, Mashhad, Iran 0000-0002-2624-7242 Journal Article 2019 08 03 Background: Synthesis of nanoparticles by biological systems is a reliable and eco-friendly process. Silver nanoparticles are applied to medical and industrial processes because of their specific properties. Because of antioxidant property of saffron plant (Crocus sativus), it is considered as a reducing agent to synthesize metal nanoparticles. The objective of this study was to investigate the ability of different parts of the saffron plant in reducing silver salt to silver nanoparticles (Ag-NPs) in various temperatures, and present or absent of light.Methods: Three aqueous extracts were prepared from different parts of the plant (stigma, stamen, and purple petal). The Ag-NP synthesize was performed at various temperatures in the present and absent of light in final concentration of 1 mM silver nitrate solution. The prepared Ag-NPs were characterized by ultraviolet-visible spectroscopy (UV-Vis). The mean particle size was investigated using dynamic light scattering (DLS).Findings: The UV-Vis spectrum showed absorption peaks at 400-460 nm. Moreover, the size range of the synthesized nanoparticles was about 15-20 nm. The red stigma and purple petal extracts produced Ag-NPs in the present of light at room temperature. In the absent of light, the absorbance was also observed for the petal extract, whereas the UV-Vis absorption was not observed in stigma extract in the absence of light at room temperature. The stamen extract produced nanoparticles at 60 0C and 80 0C in the presence and absent of light, respectively.Conclusion: Various parts of saffron flower (except green part) showed appropriate ability as reducing and capping agent for synthesis of silver nanoparticles. In addition, light and temperature seemed to have critical effects on the efficiency of plant extracts for producing nanoparticles. Background: Synthesis of nanoparticles by biological systems is a reliable and eco-friendly process. Silver nanoparticles are applied to medical and industrial processes because of their specific properties. Because of antioxidant property of saffron plant (Crocus sativus), it is considered as a reducing agent to synthesize metal nanoparticles. The objective of this study was to investigate the ability of different parts of the saffron plant in reducing silver salt to silver nanoparticles (Ag-NPs) in various temperatures, and present or absent of light.Methods: Three aqueous extracts were prepared from different parts of the plant (stigma, stamen, and purple petal). The Ag-NP synthesize was performed at various temperatures in the present and absent of light in final concentration of 1 mM silver nitrate solution. The prepared Ag-NPs were characterized by ultraviolet-visible spectroscopy (UV-Vis). The mean particle size was investigated using dynamic light scattering (DLS).Findings: The UV-Vis spectrum showed absorption peaks at 400-460 nm. Moreover, the size range of the synthesized nanoparticles was about 15-20 nm. The red stigma and purple petal extracts produced Ag-NPs in the present of light at room temperature. In the absent of light, the absorbance was also observed for the petal extract, whereas the UV-Vis absorption was not observed in stigma extract in the absence of light at room temperature. The stamen extract produced nanoparticles at 60 0C and 80 0C in the presence and absent of light, respectively.Conclusion: Various parts of saffron flower (except green part) showed appropriate ability as reducing and capping agent for synthesis of silver nanoparticles. In addition, light and temperature seemed to have critical effects on the efficiency of plant extracts for producing nanoparticles. https://jims.mui.ac.ir/article_16000_67510c8942bad17d29a67491c18d138f.pdf