Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 38 576 2020 06 21 The Effects of Aerobic Training in Polluted Air and Vitamin C Supplementation on Serum Levels of Endothelin-1 and Nitric Oxide in Wistar Rats The Effects of Aerobic Training in Polluted Air and Vitamin C Supplementation on Serum Levels of Endothelin-1 and Nitric Oxide in Wistar Rats 339 346 16076 10.22122/jims.v38i576.12581 FA Amin Eatemady-Boroujeni PhD Candidate, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, Iran 0000-0001-6815-7635 Mehdi Kargarfard Professor, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, Iran 0000-0002-9205-799X Hojatallah Alaei Professor, Department of Medical Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-9474-5756 Journal Article 2020 01 02 Background: There is little information on the effects of exercise in polluted air and vitamin C intake on plasma factors. The aim of this study was to investigate the effect of twelve weeks of aerobic training in polluted air and vitamin C supplementation on serum levels of endothelin-1 and nitric oxide in Wistar rats.Methods: 50 male Wistar rats were randomly divided into five groups of training, training-supplementation, training-pollution, training-pollution-supplementation, and control. Training protocol consisted of aerobic training for 40 minutes with intensity of 65%-80% of maximal speed for 12 weeks and 5 sessions per week. The amount of vitamin C consumed in each rat was 20 mg/kg/day used orally. Plasma levels of endothelin-1 and nitric oxide were measured at the end of the study. Data were analyzed using one-way analysis of variance.Findings: Endothelin-1 levels significantly increased in polluted air groups; vitamin C was able to decrease endothelin-1 levels (P < 0.05). The level of nitric oxide increased significantly in training in polluted air groups. These results were significantly different from the training-pollution-supplementation group (P < 0.05).Conclusion: According to the results of this study, vitamin C intake is effective in decreasing plasma levels of endothelin-1 during exposure to air pollution. The effect of exercise and polluted air on nitric oxide was significant, and vitamin C alone reduced nitric oxide in training in polluted air group. Background: There is little information on the effects of exercise in polluted air and vitamin C intake on plasma factors. The aim of this study was to investigate the effect of twelve weeks of aerobic training in polluted air and vitamin C supplementation on serum levels of endothelin-1 and nitric oxide in Wistar rats.Methods: 50 male Wistar rats were randomly divided into five groups of training, training-supplementation, training-pollution, training-pollution-supplementation, and control. Training protocol consisted of aerobic training for 40 minutes with intensity of 65%-80% of maximal speed for 12 weeks and 5 sessions per week. The amount of vitamin C consumed in each rat was 20 mg/kg/day used orally. Plasma levels of endothelin-1 and nitric oxide were measured at the end of the study. Data were analyzed using one-way analysis of variance.Findings: Endothelin-1 levels significantly increased in polluted air groups; vitamin C was able to decrease endothelin-1 levels (P < 0.05). The level of nitric oxide increased significantly in training in polluted air groups. These results were significantly different from the training-pollution-supplementation group (P < 0.05).Conclusion: According to the results of this study, vitamin C intake is effective in decreasing plasma levels of endothelin-1 during exposure to air pollution. The effect of exercise and polluted air on nitric oxide was significant, and vitamin C alone reduced nitric oxide in training in polluted air group. https://jims.mui.ac.ir/article_16076_5717490cd17470a679e7b314ba139a95.pdf