Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 41 722 2023 07 23 The Effect of Aerobic Exercise Combined with the Combination of Gallic Acid and Kaempferol on Neurogenesis Caused by the Side Effects of Paclitaxel in Mice with Breast Cancer The Effect of Aerobic Exercise Combined with the Combination of Gallic Acid and Kaempferol on Neurogenesis Caused by the Side Effects of Paclitaxel in Mice with Breast Cancer 427 437 30996 10.48305/jims.v41.i722.0427 FA Zeytoon Malekpoor PhD Student, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 0000-0002-8506-7436 Farzaneh Taghian Associate Professor, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 0000-0001-9531-2952 Khosro Jalali Dehkordi Associate Professor, Department of Sports Physiology, School of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 0000-0003-0210-5984 Journal Article 2023 03 26 <strong>Background:</strong><em> </em>The research aims to determine the effect of aerobic exercise, Gallic acid, and Kaempferol on neurogenesis caused by the side effects of the paclitaxel drug in mice with breast cancer.<br /><strong>Methods:</strong><strong> </strong>36 female BALB/c mice were randomly divided in to six groups, including the negative control group, breast cancer group (induced with 4T1 cell line injection), cancer-chemotherapy group (10 mg/kg injected paclitaxel), cancer mice treated with chemotherapy+supplement (Gallic acid and Kaempferol), cancer mice treated with chemotherapy+aerobic exercise, cancer mice treated with chemotherapy+supplement+aerobic exercise for eight weeks. The gene expressions were measured by the Real Time-PCR method. Genes were selected using bioinformatics software.<br /><strong>Findings:</strong><strong> </strong>The expression level of JAG1 was decreased in the chemotherapy group. Moreover, the expression level of JAG1 was significantly reduced by Gallic acid, Kaempferol supplements, and aerobic exercise compared with the breast cancer and cancer-chemotherapy groups. In addition, Gallic acid and Kaempferol supplements, along with aerobic exercise, significantly declined JAG1 gene expression compared with other groups. The expression levels of the BDNF and NGF genes were increased in the cancer group treated with chemotherapy+supplement and chemotherapy+aerobic exercise group. The expression genes of BDNF and NGF in the cancer group treated with chemotherapy+supplements+aerobic exercise significantly increased compared to other groups.<br /><strong>Conclusion:</strong> Aerobic exercises+supplements declined the side effects of paclitaxel and improved the neurogenesis. <strong>Background:</strong><em> </em>The research aims to determine the effect of aerobic exercise, Gallic acid, and Kaempferol on neurogenesis caused by the side effects of the paclitaxel drug in mice with breast cancer.<br /><strong>Methods:</strong><strong> </strong>36 female BALB/c mice were randomly divided in to six groups, including the negative control group, breast cancer group (induced with 4T1 cell line injection), cancer-chemotherapy group (10 mg/kg injected paclitaxel), cancer mice treated with chemotherapy+supplement (Gallic acid and Kaempferol), cancer mice treated with chemotherapy+aerobic exercise, cancer mice treated with chemotherapy+supplement+aerobic exercise for eight weeks. The gene expressions were measured by the Real Time-PCR method. Genes were selected using bioinformatics software.<br /><strong>Findings:</strong><strong> </strong>The expression level of JAG1 was decreased in the chemotherapy group. Moreover, the expression level of JAG1 was significantly reduced by Gallic acid, Kaempferol supplements, and aerobic exercise compared with the breast cancer and cancer-chemotherapy groups. In addition, Gallic acid and Kaempferol supplements, along with aerobic exercise, significantly declined JAG1 gene expression compared with other groups. The expression levels of the BDNF and NGF genes were increased in the cancer group treated with chemotherapy+supplement and chemotherapy+aerobic exercise group. The expression genes of BDNF and NGF in the cancer group treated with chemotherapy+supplements+aerobic exercise significantly increased compared to other groups.<br /><strong>Conclusion:</strong> Aerobic exercises+supplements declined the side effects of paclitaxel and improved the neurogenesis. https://jims.mui.ac.ir/article_30996_3277651248e7ccf4b6e6a8b1763508fe.pdf