Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 42 758 2024 04 20 Effects of Pomegranate Peel and Atorvastatin on the Inflammatory Factors and the Glucose Transport 4 in 3T3-L1 Cell Line Effects of Pomegranate Peel and Atorvastatin on the Inflammatory Factors and the Glucose Transport 4 in 3T3-L1 Cell Line 132 139 31202 10.48305/jims.v42.i758.0132 FA Behnoosh Etemadi Kermani MSc Student, Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran 0009-0009-6755-1337 Farzaneh Joudollah Zadeh MSc Student, Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran 0009-0004-3344-6890 Atousa Moradzadegan Assistant Professor, Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran 0000-0002-9838-3964 Journal Article 2023 09 04 <strong>Background:</strong><em> </em>Pomegranate peel extract (PPE) exhibits biological activities with potential therapeutic applications. In this research, the antioxidant properties of the combination of atorvastatin and PPE, and the effect of this combination on the regulation of inflammatory factors and the gene pathway of glucose transport regulation (GLUT4) in tissue fibroblast cells (3T3-L1). <strong>Methods:</strong><strong> </strong>This research was done in a descriptive-analytical way. The percentage of cell survival after treatment with suitable concentrations of aqueous extract of pomegranate peel (AEPP) and atorvastatin drug was obtained. The amount of inflammatory cytokines IL-1, IL-6, and TNF-α in the supernatant solution of treated cells was measured using the ELISA method. Then the antioxidant activity and GLUT4 expression were measured using real-time PCR. <strong>Findings:</strong><strong> </strong>The highest antioxidant capacity is related to the combination of AEPP with atorvastatin. Also, the level of IL-1, TGF-B, and IL-6 cytokines increased. Furthermore, atorvastatin significantly increased the level of GLUT4 in adipocytes compared to the combination of atorvastatin and AEPP concentration, as well as AEPP alone. <strong>Conclusion:</strong> The results showed that the combined use of AEPP and Atorvastatin can improve the efficiency of antioxidant pathways in the cell and significantly reduce the level of reactive oxygen. In addition, atorvastatin has a greater effect on regulating the function of inflammatory factors and the adipose glucose transporter 4 (GLUT4) regulatory gene pathway than the combination of atorvastatin and AEPP concentrations. However, more studies are needed in this area. <strong>Background:</strong><em> </em>Pomegranate peel extract (PPE) exhibits biological activities with potential therapeutic applications. In this research, the antioxidant properties of the combination of atorvastatin and PPE, and the effect of this combination on the regulation of inflammatory factors and the gene pathway of glucose transport regulation (GLUT4) in tissue fibroblast cells (3T3-L1). <strong>Methods:</strong><strong> </strong>This research was done in a descriptive-analytical way. The percentage of cell survival after treatment with suitable concentrations of aqueous extract of pomegranate peel (AEPP) and atorvastatin drug was obtained. The amount of inflammatory cytokines IL-1, IL-6, and TNF-α in the supernatant solution of treated cells was measured using the ELISA method. Then the antioxidant activity and GLUT4 expression were measured using real-time PCR. <strong>Findings:</strong><strong> </strong>The highest antioxidant capacity is related to the combination of AEPP with atorvastatin. Also, the level of IL-1, TGF-B, and IL-6 cytokines increased. Furthermore, atorvastatin significantly increased the level of GLUT4 in adipocytes compared to the combination of atorvastatin and AEPP concentration, as well as AEPP alone. <strong>Conclusion:</strong> The results showed that the combined use of AEPP and Atorvastatin can improve the efficiency of antioxidant pathways in the cell and significantly reduce the level of reactive oxygen. In addition, atorvastatin has a greater effect on regulating the function of inflammatory factors and the adipose glucose transporter 4 (GLUT4) regulatory gene pathway than the combination of atorvastatin and AEPP concentrations. However, more studies are needed in this area. https://jims.mui.ac.ir/article_31202_435ea5e88223276427a4d4613a7e06a5.pdf