Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75954067520220723The Relationship between the Gene Expression Level of MYC Gene and Non-Coding RNA Lnc-Myc-2-34 Dup1 in Acute Lymphoblastic LeukemiaThe Relationship between the Gene Expression Level of MYC Gene and Non-Coding RNA Lnc-Myc-2-34 Dup1 in Acute Lymphoblastic Leukemia4124202435610.48305/jims.v40.i675.0412FAKamalShahamiriCellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran0000-0001-7767-1484ArashAlghasiThalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0003-3482-2195NajmaldinSakiThalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0001-8494-5594Gholam AbbasKaydaniDepartment of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0003-0324-3238HosseinTeimoriCellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran0000-0002-5522-3290Journal Article20220814<strong>Background:</strong><em> </em>In recent years, an increasing trend in the incidence of acute lymphoblastic leukemia (ALL) has been reported. However, the molecular mechanisms involved are not fully understood. Because of the importance of c-MYC in ALL pathogenesis, it is important to consider the associated lncRNAs in identifying the molecular mechanisms involved in disease progression. The consequences of notch signals, depending on the dose and content can be highly pleiotropic. In hemolymphoid, notch signaling affects various cell lines at different stages of growth. The present study aimed to investigate the role of the <em>MYC</em> gene and related LncRNAs as a potential target for the treatment of acute lymphoblastic leukemia.
<strong>Methods:</strong><strong> </strong>This case-control study was performed on 40 ALL patients and 40 healthy controls during the years 2020-2021. For this purpose, total RNA was extracted from blood samples and after cDNA synthesis, <em>MYC</em>, <br />and-myc-2-34 dup1 expression was measured using Real-Time PCR. Statistical analysis of the results was performed using SPSS software and appropriate tests.
<strong>Findings:</strong><strong> </strong>The results of the gene expression study showed that in patients with ALL, <em>MYC</em> expression and related lncRNA lnc-myc-2-34 dup1 compared to controls had significant increases. These expression changes were not significantly different in age, sex, MRD, and T-ALL and B-ALL categories. lncRNA lnc-myc-2-34 dup1 correlated with the <em>MYC</em> gene, and the ROC curve indicated their potential as a strong biomarker.
<strong>Conclusion:</strong> Using lncRNAs as diagnostic, prognostic, and therapeutic markers can be an appropriate option that needs further research. According to the present study findings, the increased expression of myc-2-34 dup1 in patients with ALL has been reported for the first time thus, can be used as strong biomarkers.<strong>Background:</strong><em> </em>In recent years, an increasing trend in the incidence of acute lymphoblastic leukemia (ALL) has been reported. However, the molecular mechanisms involved are not fully understood. Because of the importance of c-MYC in ALL pathogenesis, it is important to consider the associated lncRNAs in identifying the molecular mechanisms involved in disease progression. The consequences of notch signals, depending on the dose and content can be highly pleiotropic. In hemolymphoid, notch signaling affects various cell lines at different stages of growth. The present study aimed to investigate the role of the <em>MYC</em> gene and related LncRNAs as a potential target for the treatment of acute lymphoblastic leukemia.
<strong>Methods:</strong><strong> </strong>This case-control study was performed on 40 ALL patients and 40 healthy controls during the years 2020-2021. For this purpose, total RNA was extracted from blood samples and after cDNA synthesis, <em>MYC</em>, <br />and-myc-2-34 dup1 expression was measured using Real-Time PCR. Statistical analysis of the results was performed using SPSS software and appropriate tests.
<strong>Findings:</strong><strong> </strong>The results of the gene expression study showed that in patients with ALL, <em>MYC</em> expression and related lncRNA lnc-myc-2-34 dup1 compared to controls had significant increases. These expression changes were not significantly different in age, sex, MRD, and T-ALL and B-ALL categories. lncRNA lnc-myc-2-34 dup1 correlated with the <em>MYC</em> gene, and the ROC curve indicated their potential as a strong biomarker.
<strong>Conclusion:</strong> Using lncRNAs as diagnostic, prognostic, and therapeutic markers can be an appropriate option that needs further research. According to the present study findings, the increased expression of myc-2-34 dup1 in patients with ALL has been reported for the first time thus, can be used as strong biomarkers.https://jims.mui.ac.ir/article_24356_8e8005fe9e75607ca4be6bc2cc0e1e2d.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75954067520220723Evaluation of Educational Self-Care Needs of Patients with Multiple Sclerosis: From the Perspective of Patients and ExpertsEvaluation of Educational Self-Care Needs of Patients with Multiple Sclerosis: From the Perspective of Patients and Experts4214282435710.48305/jims.v40.i675.0421FAFarinTatariAssistant Professor, Department of Public Health, Neyshabur University of Medical Sciences, Neyshabur, Iran0000-0002-9179-0225HassanEbrahimpour-SadagheyaniStudent Research Committee, Department of Health Information Technology, Neyshabur University of Medical Sciences, Neyshabur, IranJournal Article20220814<strong>Background:</strong><em> </em>Implementing a coherent multidisciplinary self-care program in patients with multiple sclerosis (MS) based on their needs can improve their health status. This study was conducted to investigate the self-care education needs in patients with MS from the perspective of experts and patients.<br /><strong>Methods:</strong><strong> </strong>The present study is a cross-sectional analytical study conducted in Neishabour in 2020. A total of 100 participants who were selected by non-probability sampling method participated in this study. Data were collected from two 22-item Likert-based researcher-made questionnaires. The validity of the content of the items was confirmed based on the Waltz and Basel index (CVI > 0.79) and the reliability of the instrument was retested with a correlation coefficient of 0.89. The questionnaires included two sections: demographics and education needs. Data were analyzed using descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (Mann-Whitney and t-test).<br /><strong>Findings:</strong><strong> </strong>With regards to the type and method of learning, 52 (86.7%) patients preferred to learn self-care educational materials electronically. According to experts, teaching effective physical exercises was most necessary, while from the patients' point of view, teaching complementary therapies and traditional medicine was most needed. The most important educational priority of patients was about how to overcome sexual problems, while according to experts, education on how to deal with intestinal and urinary disorders and management of weakness and fatigue caused by the disease had the highest priority.<br /><strong>Conclusion:</strong> The results showed the scope and content of self-care education in patients with M S differed from experts and patients point of view.<strong>Background:</strong><em> </em>Implementing a coherent multidisciplinary self-care program in patients with multiple sclerosis (MS) based on their needs can improve their health status. This study was conducted to investigate the self-care education needs in patients with MS from the perspective of experts and patients.<br /><strong>Methods:</strong><strong> </strong>The present study is a cross-sectional analytical study conducted in Neishabour in 2020. A total of 100 participants who were selected by non-probability sampling method participated in this study. Data were collected from two 22-item Likert-based researcher-made questionnaires. The validity of the content of the items was confirmed based on the Waltz and Basel index (CVI > 0.79) and the reliability of the instrument was retested with a correlation coefficient of 0.89. The questionnaires included two sections: demographics and education needs. Data were analyzed using descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (Mann-Whitney and t-test).<br /><strong>Findings:</strong><strong> </strong>With regards to the type and method of learning, 52 (86.7%) patients preferred to learn self-care educational materials electronically. According to experts, teaching effective physical exercises was most necessary, while from the patients' point of view, teaching complementary therapies and traditional medicine was most needed. The most important educational priority of patients was about how to overcome sexual problems, while according to experts, education on how to deal with intestinal and urinary disorders and management of weakness and fatigue caused by the disease had the highest priority.<br /><strong>Conclusion:</strong> The results showed the scope and content of self-care education in patients with M S differed from experts and patients point of view.https://jims.mui.ac.ir/article_24357_88894d2a720792fb60caa22d8cbeb66d.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75954067520220723The Relationship between the Length of the Greater Trochanter to the Lateral Epicondyle of the Femur Bone with the Distance between the Olecranon Type and the Finger Type of 5th HandsThe Relationship between the Length of the Greater Trochanter to the Lateral Epicondyle of the Femur Bone with the Distance between the Olecranon Type and the Finger Type of 5th Hands4294342435810.48305/jims.v40.i675.0429FAHoseinAkbari-AghdamAssistant Professor, Department of Orthopedics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-3274-2175Moahammad AliTalebi-DastenaeeStudent of Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0001-6983-4458MahshidTellooResident of Internal Medicine, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran0000-0001-7865-5961ZahraShokran-ArdakaniOperating Room Technologist, School of Nursing And Midwifery, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20220814<strong>Background:</strong><em> </em>Femoral bone fractures are one of the most important bone fractures particulary following trauma. Femur length measurements and insertion of properly sized intramedullary nail is among the major concerns of orthopedic surgeons. Nowadays the length of the femoral nail is calculated by measuring the trochanteric length of the femur to the upper patellar pole or lateral epicondyle. The aim of this study was to evaluate the relationship between the length of the greater trochanter to the lateral femoral epicondyle and the distance between the olecranon tip and the 5<sup>th</sup> fingertip.
<strong>Methods:</strong><strong> </strong>This cross-sectional study was performed on 380 outpatients (17 to 65 years old) in Kashani and Alzahra hospitals in Isfahan. The length of the olecranon tip to 5<sup>th</sup> fingertip and the length of the greater trochanter to the lateral femoral epicondyle were measured while overall the groups were gonna evaluated in terms of age, gender and body mass. T-test was used to analyze the research data for any statistical significance.
<strong>Findings:</strong><strong> </strong>Examination of the data showed that there was no significant difference between the mean length of the greater trochanter to the lateral epicondyle of the femur and the length of the olecranon to 5<sup>th</sup> fingertip. When the mean difference between the two lengths was examined separately in terms of sex and age and body mass index, similar results were obtained and no significant difference was observed.
<strong>Conclusion:</strong> There was no significant difference between the mean length of the greater trochanter to the lateral epicondyle of the femur and the length of the olecranon to 5<sup>th</sup> fingertip. There was no significant difference between the two measured lengths in different age, sex and weight groups.<strong>Background:</strong><em> </em>Femoral bone fractures are one of the most important bone fractures particulary following trauma. Femur length measurements and insertion of properly sized intramedullary nail is among the major concerns of orthopedic surgeons. Nowadays the length of the femoral nail is calculated by measuring the trochanteric length of the femur to the upper patellar pole or lateral epicondyle. The aim of this study was to evaluate the relationship between the length of the greater trochanter to the lateral femoral epicondyle and the distance between the olecranon tip and the 5<sup>th</sup> fingertip.
<strong>Methods:</strong><strong> </strong>This cross-sectional study was performed on 380 outpatients (17 to 65 years old) in Kashani and Alzahra hospitals in Isfahan. The length of the olecranon tip to 5<sup>th</sup> fingertip and the length of the greater trochanter to the lateral femoral epicondyle were measured while overall the groups were gonna evaluated in terms of age, gender and body mass. T-test was used to analyze the research data for any statistical significance.
<strong>Findings:</strong><strong> </strong>Examination of the data showed that there was no significant difference between the mean length of the greater trochanter to the lateral epicondyle of the femur and the length of the olecranon to 5<sup>th</sup> fingertip. When the mean difference between the two lengths was examined separately in terms of sex and age and body mass index, similar results were obtained and no significant difference was observed.
<strong>Conclusion:</strong> There was no significant difference between the mean length of the greater trochanter to the lateral epicondyle of the femur and the length of the olecranon to 5<sup>th</sup> fingertip. There was no significant difference between the two measured lengths in different age, sex and weight groups.https://jims.mui.ac.ir/article_24358_3548e4682a626fb125c56420344f8836.pdf