Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 42 779 2024 09 22 Investigating the Effect of a Combined Training Course on the Myogenesis Process Through the Decorin/Sparc Gene Pathway of Fast and Slow Twitch Fibers of Immature Male Rats Investigating the Effect of a Combined Training Course on the Myogenesis Process Through the Decorin/Sparc Gene Pathway of Fast and Slow Twitch Fibers of Immature Male Rats 696 702 31302 10.48305/jims.v42.i779.0696 FA Mohammad Reza Khalili Far PhD Candidate of Exercise Physiology, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran 0009-0003-4275-5348 Mohammad Reza Fadaei Chafy Assistant Professor, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran 0000-0003-0265-5329 Alireza Elmieh Associate Professor, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran 0000-0002-6266-0018 Journal Article 2024 04 25 Background: Skeletal muscle is a major endocrine organ that releases myokines and plays a pivotal role in the communication between muscle and other tissues. The present study aimed to determine the effect of a combined training course on the myogenesis process through the pathway of decorin and spark genes in the muscles of immature rats. Methods: In the present study, ten two-week-old male rats were randomly divided into two control and training groups. The resistance-aerobic training program was carried out for 6 weeks on separate days. The rats were dissected 48 hours after the last training session, and the gastrocnemius and soleus muscles were separated and used for pathological tests. Findings: Based on statistical analysis, it was found that in the training group, the expression of Spark gene (P ≤ 0.001) and decorin (P ≤ 0.001) in the soleus muscle and the expression of Spark gene (P ≤ 0.028) and decorin (P ≤ 0.001) in the gastrocnemius muscle had higher values than the control group. However, the expression values of Spark (P ≤ 0.015) and decorin (P ≤ 0.001) genes in the soleus muscle were significantly higher compared to the calf muscle. Conclusion: The results showed that myogenesis increased due to resistance-aerobic training in immature male rats, which was associated with increased expression of the decorin spark gene in both slow and fast-twitch muscle fibers. However, the expression of both spark and decorin genes in the slow-twitch fiber had higher values than the fast-twitch fibers, which indicates that this type of muscle fiber is more trainable. Background: Skeletal muscle is a major endocrine organ that releases myokines and plays a pivotal role in the communication between muscle and other tissues. The present study aimed to determine the effect of a combined training course on the myogenesis process through the pathway of decorin and spark genes in the muscles of immature rats. Methods: In the present study, ten two-week-old male rats were randomly divided into two control and training groups. The resistance-aerobic training program was carried out for 6 weeks on separate days. The rats were dissected 48 hours after the last training session, and the gastrocnemius and soleus muscles were separated and used for pathological tests. Findings: Based on statistical analysis, it was found that in the training group, the expression of Spark gene (P ≤ 0.001) and decorin (P ≤ 0.001) in the soleus muscle and the expression of Spark gene (P ≤ 0.028) and decorin (P ≤ 0.001) in the gastrocnemius muscle had higher values than the control group. However, the expression values of Spark (P ≤ 0.015) and decorin (P ≤ 0.001) genes in the soleus muscle were significantly higher compared to the calf muscle. Conclusion: The results showed that myogenesis increased due to resistance-aerobic training in immature male rats, which was associated with increased expression of the decorin spark gene in both slow and fast-twitch muscle fibers. However, the expression of both spark and decorin genes in the slow-twitch fiber had higher values than the fast-twitch fibers, which indicates that this type of muscle fiber is more trainable.

https://jims.mui.ac.ir/article_31302_2cf7926aeec52fbe4f1a6ae2a1770329.pdf

Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 42 779 2024 09 22 Investigating the Relationship between Sleep Quality and Pain Perception in Patients with Rheumatoid Arthritis Investigating the Relationship between Sleep Quality and Pain Perception in Patients with Rheumatoid Arthritis 703 710 31300 10.48305/jims.v42.i779.0703 FA Mahdi Rezazadeh Medical Student, Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 0009-0009-3021-0117 Mohammad Amini PhD Student, Department of Neuroscience and Addiction Studies, Sa'ad Brain Studies Association, Student Scientific Research Center(SSRC), School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 0000-0001-7367-037X Fatemeh Toufan Assistant Professor, Department of Clinical Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 0000-0001-6380-5461 Zahra Yousefi Assistant Professor School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran 0000-0002-2114-0964 Journal Article 2024 06 01 Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. Patients with RA experience a lower quality of life due to joint damage and associated limitations in social activities and physical functions. These patients often have poor sleep quality, which may affect other aspects, including pain perception. This study aims to investigate the relationship between sleep quality and pain perception in these patients. Methods: This cross-sectional study was conducted on RA patients attending Imam Hussain Hospital, Shahroud, Iran. 137 RA patients were selected and asked to respond to relevant questions (Petersburg Sleep Quality Questionnaire, reliability 0.83, and McGill Pain Questionnaire, reliability 0.83 to 0.87). Descriptive and inferential statistics employed for data analysis. The normality of the data was assessed using the Kolmogorov-Smirnov test. Findings: The data indicated a statistically significant relationship between sleep quality and disease duration (P < 0.001), as well as between pain perception and disease duration (P > 0.02). However, no significant correlation was observed between pain perception and age or gender or between sleep quality and gender or age. The results of the Chi-square Test revealed a statistically significant correlation between sleep quality and pain perception in RA patients (P< 0.001). Conclusion: The results of the present study indicated a significant relationship between sleep quality and pain perception in patients with RA. These patients experience poorer sleep quality and greater pain perception with an increase in the duration of their disease. Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. Patients with RA experience a lower quality of life due to joint damage and associated limitations in social activities and physical functions. These patients often have poor sleep quality, which may affect other aspects, including pain perception. This study aims to investigate the relationship between sleep quality and pain perception in these patients. Methods: This cross-sectional study was conducted on RA patients attending Imam Hussain Hospital, Shahroud, Iran. 137 RA patients were selected and asked to respond to relevant questions (Petersburg Sleep Quality Questionnaire, reliability 0.83, and McGill Pain Questionnaire, reliability 0.83 to 0.87). Descriptive and inferential statistics employed for data analysis. The normality of the data was assessed using the Kolmogorov-Smirnov test. Findings: The data indicated a statistically significant relationship between sleep quality and disease duration (P < 0.001), as well as between pain perception and disease duration (P > 0.02). However, no significant correlation was observed between pain perception and age or gender or between sleep quality and gender or age. The results of the Chi-square Test revealed a statistically significant correlation between sleep quality and pain perception in RA patients (P< 0.001). Conclusion: The results of the present study indicated a significant relationship between sleep quality and pain perception in patients with RA. These patients experience poorer sleep quality and greater pain perception with an increase in the duration of their disease.

https://jims.mui.ac.ir/article_31300_c7602768a214451d3d91346fed37176b.pdf

Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 42 779 2024 09 22 The Effect of Exposure to Perfluorooctanoic Acid During Pregnancy on the Expression of Brain-Derived Neurotrophic Factor (BDNF) in the Newborn Rat Brain The Effect of Exposure to Perfluorooctanoic Acid During Pregnancy on the Expression of Brain-Derived Neurotrophic Factor (BDNF) in the Newborn Rat Brain 711 717 31301 10.48305/jims.v42.i779.0711 FA Satar Doghi MSc Student, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Zeinolabedin Sharifian Dastjerdi Assistant Professor, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Nazem Ghasemi Associate Professor, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-6729-0271 Journal Article 2024 07 10 Background: Following exposure to toxic agents, structural and functional changes occur in neurons. These changes can lead to the nervous system dysfunction, such as sensorimotor disorders. In the current study, we investigated how exposure to perfluorooctanoic acid during pregnancy affects the expression of brain-derived neurotrophic factors in the brains of newborn rats. Methods: The brains of newborn Wistar rats which divided into five groups include control, sham and three PFOA receiving groups were used. In the PFOA groups, this compound was gavage with a dose of 1, 5 and 10 mg daily. The brains of newborn mice were removed 20 days after birth and the level of neurotrophic factors derived from these samples was evaluated using ELISA and Real Time PCR methods. Findings: The results showed that the mean expression of the BDNF gene significantly increased in the PFOA-receiving groups compared to other groups (P ≤ 0.001). Also, there was a significant increase in BDNF protein expression in the groups that received doses of 5 and 10 mg of PFOA compared to the control and sham groups (P ≤ 0.05). Conclusion: The results of this study showed that exposure to a natural pollutant such as PFOA can lead to an increase in BDNF expression, and this increase is probably due to the prevention of the destructive effects of PFOA on the development of the nervous system. Therefore, it is recommended to minimize exposure to PFOA-containing sources as much as possible during pregnancy. Background: Following exposure to toxic agents, structural and functional changes occur in neurons. These changes can lead to the nervous system dysfunction, such as sensorimotor disorders. In the current study, we investigated how exposure to perfluorooctanoic acid during pregnancy affects the expression of brain-derived neurotrophic factors in the brains of newborn rats. Methods: The brains of newborn Wistar rats which divided into five groups include control, sham and three PFOA receiving groups were used. In the PFOA groups, this compound was gavage with a dose of 1, 5 and 10 mg daily. The brains of newborn mice were removed 20 days after birth and the level of neurotrophic factors derived from these samples was evaluated using ELISA and Real Time PCR methods. Findings: The results showed that the mean expression of the BDNF gene significantly increased in the PFOA-receiving groups compared to other groups (P ≤ 0.001). Also, there was a significant increase in BDNF protein expression in the groups that received doses of 5 and 10 mg of PFOA compared to the control and sham groups (P ≤ 0.05). Conclusion: The results of this study showed that exposure to a natural pollutant such as PFOA can lead to an increase in BDNF expression, and this increase is probably due to the prevention of the destructive effects of PFOA on the development of the nervous system. Therefore, it is recommended to minimize exposure to PFOA-containing sources as much as possible during pregnancy.

https://jims.mui.ac.ir/article_31301_61d427b812eea882235a3ae3ce613dd2.pdf