Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 43 802 2025 03 21 Investigation of the Healing Effects of Hydroethanolic Extract of Heracleum Persicum on Full-thickness Incisional Cutaneous Wounds in Rats Investigation of the Healing Effects of Hydroethanolic Extract of Heracleum Persicum on Full-thickness Incisional Cutaneous Wounds in Rats 30 45 31389 10.48305/jims.v43.i802.0030 FA Ali Davoudi Graduated in Veterinary Medicine, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran 0009-0001-1645-1485 Fatemeh Hoseinpour Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran 0000-0003-4869-0242 Mohammad Hashemnia Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran 0000-0002-2899-4794 Journal Article 2024 10 17 Background: Wound healing is the body's natural repair process after injury. Due to its high antioxidant capacity and many effective components, this study aimed to assess the impact of topically applied Heracleum persicum extract on rat full-thickness incisional cutaneous wound healing. Methods: After induction the wound, sixty male rats were divided into four groups: control, basal cream, H. persicum 5% and 10% and were treated daily with saline, basal cream, H. persicum 5% and H. persicum 10% for 14 days, respectively. The animals were euthanized at 7, 14 and 21 days post-injury, and samples were collected for histopathological and biochemical analysis. Findings: A prominent decrease in the wound area was observed in the treatment groups, particularly H. persicum 10% compared to the control group. Additionally, treatment with H. persicum decreased the number of lymphocytes and improved the number of fibroblasts at the earlier stages and increased the number of fibrocytes at the later stages of wound healing. Also, re-epithelization, tissue alignment, higher maturity of the collagen fibers and large capillary-sized blood vessels revealed significant changes in the treatment groups, especially H. persicum 10% compared to the control group. H. persicum extracts increased hydroxyproline and glycosaminoglycans contents and elevated total antioxidant capacity, while decreased malondialdehyde compared to the control group. Conclusion: This study showed that H. persicum promotes wound healing activity. This property can be attributed to its action on blood vessels, modulation of inflammation, its role in initiating more collagen and glycosaminoglycan production and decreasing oxidative stress. Background: Wound healing is the body's natural repair process after injury. Due to its high antioxidant capacity and many effective components, this study aimed to assess the impact of topically applied Heracleum persicum extract on rat full-thickness incisional cutaneous wound healing. Methods: After induction the wound, sixty male rats were divided into four groups: control, basal cream, H. persicum 5% and 10% and were treated daily with saline, basal cream, H. persicum 5% and H. persicum 10% for 14 days, respectively. The animals were euthanized at 7, 14 and 21 days post-injury, and samples were collected for histopathological and biochemical analysis. Findings: A prominent decrease in the wound area was observed in the treatment groups, particularly H. persicum 10% compared to the control group. Additionally, treatment with H. persicum decreased the number of lymphocytes and improved the number of fibroblasts at the earlier stages and increased the number of fibrocytes at the later stages of wound healing. Also, re-epithelization, tissue alignment, higher maturity of the collagen fibers and large capillary-sized blood vessels revealed significant changes in the treatment groups, especially H. persicum 10% compared to the control group. H. persicum extracts increased hydroxyproline and glycosaminoglycans contents and elevated total antioxidant capacity, while decreased malondialdehyde compared to the control group. Conclusion: This study showed that H. persicum promotes wound healing activity. This property can be attributed to its action on blood vessels, modulation of inflammation, its role in initiating more collagen and glycosaminoglycan production and decreasing oxidative stress.

https://jims.mui.ac.ir/article_31389_edbd47c8e4cabdf0b4e1e7f1069a226b.pdf

Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 43 802 2025 03 21 Investigation of the Protective and Anti-Inflammatory Effects of Fisetin on Serum Levels of Interferon- Gamma and Myelin Basic Protein Expressing Cells Survival in the Mouse Brain Following Cuprisone Gavage Investigation of the Protective and Anti-Inflammatory Effects of Fisetin on Serum Levels of Interferon- Gamma and Myelin Basic Protein Expressing Cells Survival in the Mouse Brain Following Cuprisone Gavage 46 52 31391 10.48305/jims.v43.i802.0046 FA Armina Bahador MSc Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0009-0002-0836-7109 Ebrahim Esfandiari Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-6436-5672 Nazem Ghasemi Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0002-6729-0271 Journal Article 2024 12 14 Background: External toxic agents with nuclear antigens similar to myelin basic proteins can activate immune cells and destroy the myelin sheath. Fisetin is vital in preventing nerve damage because of its neuroprotective effects. In the present study, the effects of fisetin were investigated on the interferon-gamma (IFN-Ɣ) serum levels and prevention of Mbp-expressing cell death in the mouse brain. Methods: 20 C57BL/6 mice were divided into five groups: control, sham, cuprizone, fisetin, and cuprizone/fisetin. Cuprizone was used to induce the death of MBP-positive cells. At the end of the study, the serum level of Interferon-gamma was determined by ELISA, and the average percentage of Mbp-positive cells was determined by immunohistochemistry technique. Finally, the data were analyzed using the One-Way ANOVA test Findings: The mean expression of the inflammatory factor Interferon-gamma in the fisetin-receiving group was significantly reduced compared to the cuprizone group (P ≤ 0.001). Also, following the use of cuprizone, the mean percentage of Mbp-positive cells in the cuprizone group was significantly reduced compared to the other groups (P ≤ 0.05). Conclusion: Cuprizone can increase the death of neuroglial cells expressing the Mbp factor by raising serum levels of the inflammatory factor Interferon-gamma. The results of this study indicate that fisetin, as a type of flavonoid, can play a crucial role in protecting neuroglial cells by exerting anti-inflammatory and neuroprotective effects and reducing the destructive effects of inflammatory factors on nervous tissue. Background: External toxic agents with nuclear antigens similar to myelin basic proteins can activate immune cells and destroy the myelin sheath. Fisetin is vital in preventing nerve damage because of its neuroprotective effects. In the present study, the effects of fisetin were investigated on the interferon-gamma (IFN-Ɣ) serum levels and prevention of Mbp-expressing cell death in the mouse brain. Methods: 20 C57BL/6 mice were divided into five groups: control, sham, cuprizone, fisetin, and cuprizone/fisetin. Cuprizone was used to induce the death of MBP-positive cells. At the end of the study, the serum level of Interferon-gamma was determined by ELISA, and the average percentage of Mbp-positive cells was determined by immunohistochemistry technique. Finally, the data were analyzed using the One-Way ANOVA test Findings: The mean expression of the inflammatory factor Interferon-gamma in the fisetin-receiving group was significantly reduced compared to the cuprizone group (P ≤ 0.001). Also, following the use of cuprizone, the mean percentage of Mbp-positive cells in the cuprizone group was significantly reduced compared to the other groups (P ≤ 0.05). Conclusion: Cuprizone can increase the death of neuroglial cells expressing the Mbp factor by raising serum levels of the inflammatory factor Interferon-gamma. The results of this study indicate that fisetin, as a type of flavonoid, can play a crucial role in protecting neuroglial cells by exerting anti-inflammatory and neuroprotective effects and reducing the destructive effects of inflammatory factors on nervous tissue.

https://jims.mui.ac.ir/article_31391_4b997c95a3cd22f4f5a45903bc4f319a.pdf

Isfahan University of Medical Sciences Journal of Isfahan Medical School 1027-7595 43 802 2025 03 21 Determining the Passing Score as A Strategy to Achieve Justice in Assessment: A Review Study Determining the Passing Score as A Strategy to Achieve Justice in Assessment: A Review Study 53 62 31390 10.48305/jims.v43.i802.0053 FA Atefeh Heidari MSc Student, Department of Medical Education, Center for Studies and Development of Medical Education, Isfahan University of Medical Sciences, Isfahan, Iran 0009-0001-1639-8450 Shahram Shayan Assistant Professor, Department of Medical Education, Center for Studies and Development of Medical Education, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-8578-9538 Journal Article 2024 11 24 Background: One of the challenging issues in education is establishing fairness in student evaluation. Determining a fixed passing score for all tests without considering the specific characteristics of each test and test taker is unfair. The passing standard for each test should be determined based on factors such as the level of difficulty of the test, the ability of the students, the learning environment, and educational goals to establish fairness. This study was conducted to provide solutions for accurately determining the passing score threshold and improving the quality of tests. It is expected that the results of this study can help test administrators and university faculty members to improve the evaluation process and take an influential step toward establishing educational fairness. Methods: This study was a narrative review and systematic search. Published Persian and English articles were searched in databases such as PubMed, Google Scholar, Scopus, and SID using keywords including standard setting, passing score, minimum pass level, and cut point. Finally, 37 studies were included in the study based on the inclusion and exclusion criteria. Findings: The articles included in the study described 16 methods for determining the passing score. These were broadly divided into four categories: test-centered, examinee-centered, performance-centered, and outcome-centered. Conclusion: To improve the quality of their exams, universities should monitor this process by utilizing experts in the evaluation and using mixed methods or item mapping as a standard approach to achieving fairness in assessments. Background: One of the challenging issues in education is establishing fairness in student evaluation. Determining a fixed passing score for all tests without considering the specific characteristics of each test and test taker is unfair. The passing standard for each test should be determined based on factors such as the level of difficulty of the test, the ability of the students, the learning environment, and educational goals to establish fairness. This study was conducted to provide solutions for accurately determining the passing score threshold and improving the quality of tests. It is expected that the results of this study can help test administrators and university faculty members to improve the evaluation process and take an influential step toward establishing educational fairness. Methods: This study was a narrative review and systematic search. Published Persian and English articles were searched in databases such as PubMed, Google Scholar, Scopus, and SID using keywords including standard setting, passing score, minimum pass level, and cut point. Finally, 37 studies were included in the study based on the inclusion and exclusion criteria. Findings: The articles included in the study described 16 methods for determining the passing score. These were broadly divided into four categories: test-centered, examinee-centered, performance-centered, and outcome-centered. Conclusion: To improve the quality of their exams, universities should monitor this process by utilizing experts in the evaluation and using mixed methods or item mapping as a standard approach to achieving fairness in assessments.

https://jims.mui.ac.ir/article_31390_452dee7ed3aac40e980602f275eec5e5.pdf