Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622IndexIndex14630FAJournal Article20151006Click to download the index of this issue.Click to download the index of this issue.https://jims.mui.ac.ir/article_14630_f0b875eb6cff6fd5f491e6b6521c7510.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622Epidemiology and Clinical Features of Cutaneous Leishmaniasis in Badrood City, Iran, in 2013Epidemiology and Clinical Features of Cutaneous Leishmaniasis in Badrood City, Iran, in 201367668414631FAMansoorehMomen-HeraviAssociate Professor, Social Determinants of Health (SDH) Research Center, Kashan University of Medical Sciences, Kashan, IranHasanAfzaliAssociate Professor, Department of Infectious Diseases, School of Medicine, Kashan University of Medical Sciences, Kashan, IranHasanaliAhmadiPediatrician, Isfahan University of Medical Sciences, Isfahan AND Badrood Hospital, Badrood, IranMaryamSaboori-KashaniNurse, Kashan University of Medical Sciences, Kashan, IranJournal Article20150203Background: Cutaneous Leishmaniasis (CL) is a common endemic parasitic disease in Iran. This disease is always a serious health problem in Isfahan province, especially in Badrood city, and its prevalence has been doubled over the last decade. This study was designed to determine the epidemiology and clinical features of cutaneous leishmaniasis in Badrood city in 2013.Methods: This descriptive cross-sectional study was done on all detected patients with cutaneous leishmaniasis in Badrood city via active detection during one year. Patients were visited by physician and a questionnaire including the demographic data and characteristics of the wounds were filled through interview and examination of patients. Diagnosis was confirmed via revealing of the leishman body in smear of the wounds. The results were analyzed using SPSS software.Findings: 55.6% of the patients were men. Age group of 15-30 years had the highest rate (23.8%) among the patients. 66.7% of patients had history of staying in Agha Ali Abbas region. All the wounds were of the rural form. The lesions of cutaneous leishmaniasis were painless, pruritus, less than 3 cm, and with secretion. Extremities were the most common sites for the infection. There was complete or partial healing after the treatment in most of the patients.Conclusion: Based on the findings of this study, cutaneous leishmaniasis in Badrood city is rural form and most of the patient are men and older than 15 years of age. Complete or partial healing can be seen after the treatment in most of the patients.Background: Cutaneous Leishmaniasis (CL) is a common endemic parasitic disease in Iran. This disease is always a serious health problem in Isfahan province, especially in Badrood city, and its prevalence has been doubled over the last decade. This study was designed to determine the epidemiology and clinical features of cutaneous leishmaniasis in Badrood city in 2013.Methods: This descriptive cross-sectional study was done on all detected patients with cutaneous leishmaniasis in Badrood city via active detection during one year. Patients were visited by physician and a questionnaire including the demographic data and characteristics of the wounds were filled through interview and examination of patients. Diagnosis was confirmed via revealing of the leishman body in smear of the wounds. The results were analyzed using SPSS software.Findings: 55.6% of the patients were men. Age group of 15-30 years had the highest rate (23.8%) among the patients. 66.7% of patients had history of staying in Agha Ali Abbas region. All the wounds were of the rural form. The lesions of cutaneous leishmaniasis were painless, pruritus, less than 3 cm, and with secretion. Extremities were the most common sites for the infection. There was complete or partial healing after the treatment in most of the patients.Conclusion: Based on the findings of this study, cutaneous leishmaniasis in Badrood city is rural form and most of the patient are men and older than 15 years of age. Complete or partial healing can be seen after the treatment in most of the patients.https://jims.mui.ac.ir/article_14631_67b3d697f52f61a5aae9588726d18edc.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622The Inhibitory Effect of Hydroalcoholic Extract of Black Punica Granatum Pericarp on the Number of Endothelial Progenitor Cells (EPCs) in Melanoma through Peroxisome Proliferator-Activated Receptor-α and γ (PPAR-α and γ) Pathways in C57BL6 MiceThe Inhibitory Effect of Hydroalcoholic Extract of Black Punica Granatum Pericarp on the Number of Endothelial Progenitor Cells (EPCs) in Melanoma through Peroxisome Proliferator-Activated Receptor-α and γ (PPAR-α and γ) Pathways in C57BL6 Mice68569314632FASimaSeifabadiApplied Physiology Research Center AND Department of Physiology, School of Medicine AND Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, IranLalehRafieeApplied Physiology Research Center AND Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranHajarNaji-EsfahaniApplied Physiology Research Center AND Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranShaghayeghHaghjooy-JavanmardAssociate Professor, Applied Physiology Research Center AND Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20150226Background: Endothelial progenitor cells (EPCs) play an important role in tumor growth and angiogenesis. Punica granatum, from Punicaceae family, has demonstrated anticancer effects in different types of cancers. This study aimed to determine the effect of hydroalcoholic extract of pomegranate peel on the number of EPCs in mouse model of melanoma and also to determine its mechanism of action.Methods: The hydroalcoholic extract of black pomegranate pericarp was prepared using 70% ethanol containing 1% acetic acid. 1 × 106 B16F10 melanoma cells were injected to each of 88 C57BL6 male mice weighing 25 g on the day 0, subcutaneously (s.c). On 7th day, mice were randomly divided into 11 groups of 8 animals. The first group (control) received distilled water. Groups second to fifth from the seventh day of the study received 50, 100, 200 and 400 mg/kg of standardized polyphenon E (PPE) via gavage. The sixth group received PPE and peroxisome proliferator-activated receptor gamma (PPAR-γ) antagonist (5 mg/kg/day) intraperitoneally. The seventh group received PPE and PPAR-α antagonist (10 mg/kg/day) intraperitoneally. Eighth and ninth groups received fenofibrate (100 mg/kg) and rosiglitazone (100 mg/kg) as agonists of PPAR-α and PPAR-γ, respectively, via gavage. Tenth and eleventh groups received PPAR antagonists, merely. On 16th day, mice were euthanized and were bled through heart puncture for EPC enumeration via flow cytometry. In flow cytometry, CD34+/VEGFR2+ cells were enumerated.Findings: PPE dose-dependently decreased the number of EPCs (P < 0.05). Number of EPCs in the groups which received fenofibrate and rosiglitazone was less than the group that received the highest dose of PPE (P < 0.05). Moreover, EPCs in the groups which received both PPAR antagonists and PPE, was more than the group which received PPE (P < 0.01).Conclusion: In conclusion, we demonstrated that PPE is effective in reducing the number of EPCs and part of this effect is through modulation of PPAR pathway.Background: Endothelial progenitor cells (EPCs) play an important role in tumor growth and angiogenesis. Punica granatum, from Punicaceae family, has demonstrated anticancer effects in different types of cancers. This study aimed to determine the effect of hydroalcoholic extract of pomegranate peel on the number of EPCs in mouse model of melanoma and also to determine its mechanism of action.Methods: The hydroalcoholic extract of black pomegranate pericarp was prepared using 70% ethanol containing 1% acetic acid. 1 × 106 B16F10 melanoma cells were injected to each of 88 C57BL6 male mice weighing 25 g on the day 0, subcutaneously (s.c). On 7th day, mice were randomly divided into 11 groups of 8 animals. The first group (control) received distilled water. Groups second to fifth from the seventh day of the study received 50, 100, 200 and 400 mg/kg of standardized polyphenon E (PPE) via gavage. The sixth group received PPE and peroxisome proliferator-activated receptor gamma (PPAR-γ) antagonist (5 mg/kg/day) intraperitoneally. The seventh group received PPE and PPAR-α antagonist (10 mg/kg/day) intraperitoneally. Eighth and ninth groups received fenofibrate (100 mg/kg) and rosiglitazone (100 mg/kg) as agonists of PPAR-α and PPAR-γ, respectively, via gavage. Tenth and eleventh groups received PPAR antagonists, merely. On 16th day, mice were euthanized and were bled through heart puncture for EPC enumeration via flow cytometry. In flow cytometry, CD34+/VEGFR2+ cells were enumerated.Findings: PPE dose-dependently decreased the number of EPCs (P < 0.05). Number of EPCs in the groups which received fenofibrate and rosiglitazone was less than the group that received the highest dose of PPE (P < 0.05). Moreover, EPCs in the groups which received both PPAR antagonists and PPE, was more than the group which received PPE (P < 0.01).Conclusion: In conclusion, we demonstrated that PPE is effective in reducing the number of EPCs and part of this effect is through modulation of PPAR pathway.https://jims.mui.ac.ir/article_14632_5e83596334400305514565ee16b9106d.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622Investigating the Serum Levels of Glutathione and Iron and the Activity of Caspase 3 in Patients with Hashimoto’s ThyroiditisInvestigating the Serum Levels of Glutathione and Iron and the Activity of Caspase 3 in Patients with Hashimoto’s Thyroiditis69470214633FAHodaRashidianMSc Student, Department of Biochemistry, Islamic Azad University, Falavarjan Branch, Isfahan, IranKahinShahanipourAssistant Professor, Department of Biochemistry, Islamic Azad University, Falavarjan Branch, Isfahan, IranJournal Article20150226Background: Considering the prevalence of thyroid dysfunction and diseases such as Hashimoto's thyroiditis and Graves' disease in the Dezful city, Iran, this study aimed to evaluate the effect of Hashimoto's thyroiditis on serum levels of glutathione and iron and the activity of caspase-3.Methods: In this case-control study, 44 patients with Hashimoto's thyroiditis and 44 healthy controls were studied. The serum levels of glutathione and iron and the activity of caspase-3 were measured. The results were analyzed using Mann-Whitney test via SPSS software.Findings: The amount of serum Fe was significantly lower in patients (P = 0.041). The glutathione level in healthy people was lower; but tests showed no significant difference (P = 0.502). The caspase-3 enzyme activity in healthy subjects was lower; but Mann-Whitney test showed no significant difference (P = 0.089).Conclusion: In patients with Hashimoto's thyroiditis in hypothyroidism phase, the serum iron level was significantly lower than the control group. In addition, the mean serum glutathione level wsa not significantly different between the groups.Background: Considering the prevalence of thyroid dysfunction and diseases such as Hashimoto's thyroiditis and Graves' disease in the Dezful city, Iran, this study aimed to evaluate the effect of Hashimoto's thyroiditis on serum levels of glutathione and iron and the activity of caspase-3.Methods: In this case-control study, 44 patients with Hashimoto's thyroiditis and 44 healthy controls were studied. The serum levels of glutathione and iron and the activity of caspase-3 were measured. The results were analyzed using Mann-Whitney test via SPSS software.Findings: The amount of serum Fe was significantly lower in patients (P = 0.041). The glutathione level in healthy people was lower; but tests showed no significant difference (P = 0.502). The caspase-3 enzyme activity in healthy subjects was lower; but Mann-Whitney test showed no significant difference (P = 0.089).Conclusion: In patients with Hashimoto's thyroiditis in hypothyroidism phase, the serum iron level was significantly lower than the control group. In addition, the mean serum glutathione level wsa not significantly different between the groups.https://jims.mui.ac.ir/article_14633_6349ccd8e98a367ae2eba1acfa755850.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622The Effects of Simultaneous Administration of Granulocyte Colony-Stimulating Factor and Interferon-alpha on OsteoporosisThe Effects of Simultaneous Administration of Granulocyte Colony-Stimulating Factor and Interferon-alpha on Osteoporosis70371314634FARoyaLariAssistant Professor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, IranSimaJahan-BakhshiMSc Student, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, IranElahehMohammadianMSc Student, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, IranAliMirshahiAssistant Professor, Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranNasserMahdavi-ShahriProfessor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, IranAliMoghimiProfessor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, IranJournal Article20150225Background: Bone loss is one of the major pathological symptoms of rheumatoid arthritis (RA) and some types of cancers. Granulocyte colony-stimulating factor (G-CSF) and interferon-alpha (IFN-α) are widely used in patients with cancer as a drug and o have been known to play a key role in rheumatoid arthritis. This study aimed to investigate the effects of these factors on osteoporosis.Methods: 28 mouse (Mus musculus, male, and eight weeks of age) were divided into 4 groups of 7. They were injected subcutaneously with distilled water as control, 10 mg/kg IFN-α and 200 mg/kg G-CSF with or without 10 mg/kg IFN-α per day, for 28 days. After killing mice, standardized radiographs were taken from femur bones and the Image J program was used for measurement of the femoral bone density. The total femoral bone volume was measured using multidetector computed tomography (MDCT).Findings: G-CSF reduced the density of the head and greater trochanter of femural bone in mice. The IFN-a alone increased bone density in the greater trochanter and the volume of the trabecular bone. This factor, in combination with G-CSF, strongly enhanced bone density and increased the volume of the trabecular bone.Conclusion: Therefore, administration of IFN-a may inhibit G-CSF-induced bone loss in inflammatory and treatment conditions.Background: Bone loss is one of the major pathological symptoms of rheumatoid arthritis (RA) and some types of cancers. Granulocyte colony-stimulating factor (G-CSF) and interferon-alpha (IFN-α) are widely used in patients with cancer as a drug and o have been known to play a key role in rheumatoid arthritis. This study aimed to investigate the effects of these factors on osteoporosis.Methods: 28 mouse (Mus musculus, male, and eight weeks of age) were divided into 4 groups of 7. They were injected subcutaneously with distilled water as control, 10 mg/kg IFN-α and 200 mg/kg G-CSF with or without 10 mg/kg IFN-α per day, for 28 days. After killing mice, standardized radiographs were taken from femur bones and the Image J program was used for measurement of the femoral bone density. The total femoral bone volume was measured using multidetector computed tomography (MDCT).Findings: G-CSF reduced the density of the head and greater trochanter of femural bone in mice. The IFN-a alone increased bone density in the greater trochanter and the volume of the trabecular bone. This factor, in combination with G-CSF, strongly enhanced bone density and increased the volume of the trabecular bone.Conclusion: Therefore, administration of IFN-a may inhibit G-CSF-induced bone loss in inflammatory and treatment conditions.https://jims.mui.ac.ir/article_14634_54af6860114f54728b5c2fd9b5cfeca9.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953333420150622Nutritional Management in Non-Alcoholic Fatty Liver Disease (NAFLD)Nutritional Management in Non-Alcoholic Fatty Liver Disease (NAFLD)71472114635FASepidehMahboobiFood Security Research Centre AND Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical sciences, Isfahan, IranRezaRouzbahaniAssistant Professor, Department of Community Medicine, School of Medicine, Isfahan University of Medical sciences, Isfahan, IranJournal Article20150616Nutritional Management in Non-Alcoholic Fatty Liver Disease (NAFLD)Nutritional Management in Non-Alcoholic Fatty Liver Disease (NAFLD)https://jims.mui.ac.ir/article_14635_e2299ee16dcb4ea787ba9f9e8396a26e.pdf