Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923IndexIndex14696FAJournal Article20151217Click to download the index of this issue.Click to download the index of this issue.https://jims.mui.ac.ir/article_14696_f74d6ef882234fd34400a296b1da6149.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923Effects of Estrogen on the Expression of Neural Markers in Differentiated Adipose-Derived Stem CellsEffects of Estrogen on the Expression of Neural Markers in Differentiated Adipose-Derived Stem Cells1239124914697FAShahnazRazaviProfessor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranNafisehAhmadiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranMohammadKazemiPhD Student, Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranHamid RezaSadeghianStudent of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20150311Background: Over the past decade, researchers have used the existing knowledge about pathways messaging protocols to successfully stimulate stem cells to generate neurons. Due to the ease of access to adipose tissue-obtained stem cells rather than other sources, whereas estrogen factor could be used to improve neural differentiation, antilogous transplantation of differentiated neurons would be widely used for certain degenerative neurological diseases such as Parkinson's disease and spinal cord injuries. The purpose of this study was to evaluate the effect of estrogen on expression of microtubule-associated protein-2 (MAP2), glial fibrillary acidic protein (GFAP) and Nestin markers.Methods: After the isolation of stem cells from adipose tissue, the neural induction was carried out through neurosphere construction; then, final differentiation of the cells was performed. Neurosphere-singed cell were transferred to neural induction medium (control group). In the estrogen-treated group, estrogen was added to the culture medium until the end of the day of distinction. Then, evaluation of the expression of neural markers, was performed using reverse transcription-polymerase chain reaction (RT-PCR) technique. In addition, MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was performed to assess cell viability.Findings: The mean expression of GFAP and Nestin markers were down regulated in treated group compared to controls (P < 0.05). The difference between the mean of MAP2 expression was not significant between the two groups. In addition, the difference between the mean of cell viability was not significant between two groups, too.Conclusion: In this study, we found that estrogen can decrease the expression of neuronal markers. However, to determine the effect of estrogen on neurogenic differentiation of stem cells, next studies should be done broader using other precise techniques.Keywords: Estrogen, Neurogenesis, Adipose-derived stem cell, Reverse transcription-polymerase chain reaction (RT-PCR)Background: Over the past decade, researchers have used the existing knowledge about pathways messaging protocols to successfully stimulate stem cells to generate neurons. Due to the ease of access to adipose tissue-obtained stem cells rather than other sources, whereas estrogen factor could be used to improve neural differentiation, antilogous transplantation of differentiated neurons would be widely used for certain degenerative neurological diseases such as Parkinson's disease and spinal cord injuries. The purpose of this study was to evaluate the effect of estrogen on expression of microtubule-associated protein-2 (MAP2), glial fibrillary acidic protein (GFAP) and Nestin markers.Methods: After the isolation of stem cells from adipose tissue, the neural induction was carried out through neurosphere construction; then, final differentiation of the cells was performed. Neurosphere-singed cell were transferred to neural induction medium (control group). In the estrogen-treated group, estrogen was added to the culture medium until the end of the day of distinction. Then, evaluation of the expression of neural markers, was performed using reverse transcription-polymerase chain reaction (RT-PCR) technique. In addition, MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was performed to assess cell viability.Findings: The mean expression of GFAP and Nestin markers were down regulated in treated group compared to controls (P < 0.05). The difference between the mean of MAP2 expression was not significant between the two groups. In addition, the difference between the mean of cell viability was not significant between two groups, too.Conclusion: In this study, we found that estrogen can decrease the expression of neuronal markers. However, to determine the effect of estrogen on neurogenic differentiation of stem cells, next studies should be done broader using other precise techniques.Keywords: Estrogen, Neurogenesis, Adipose-derived stem cell, Reverse transcription-polymerase chain reaction (RT-PCR)https://jims.mui.ac.ir/article_14697_d0ec7deb55fed8949efb61cf1fa39004.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923Evaluating T-Cell Immunoglobulin Mucin-3 (TIM-3) Receptor in the Growth Inhibition of Acute Lymphoblastic Leukemia (ALL) Cell LinesEvaluating T-Cell Immunoglobulin Mucin-3 (TIM-3) Receptor in the Growth Inhibition of Acute Lymphoblastic Leukemia (ALL) Cell Lines1250126014698FANargesZargar-BalajamMSc Student, Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranMahdiShabaniAssistant Professor, Monoclonal Antibody Research Center, Avicenna Research Institute, The Academic Center for Education, Culture and Research (ACECR), Tehran, IranMansorehHaghighiPhD Student, Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranAhmad AliBayatMonoclonal Antibody Research Center, Avicenna Research Institute, The Academic Center for Education, Culture and Research (ACECR), Tehran, IranMahmoudAghaeiAssistant Professor, Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20150506Background: T-cell immunoglobulin-mucin (TIM) is a cell-surface and transmembrane glycoprotein. TIM-3 plays a pivotal role in proliferation, invasion and metastasis of tumor cells. The present study was designed to evaluate the expressions of the TIM-3 and the role of the galectin-9, as TIM-3 ligand, in the regulation of cell proliferation in human acute lymphoblastic leukemia (ALL) cell lines.Methods: The expression level of TIM-3 was examined in the Jurkat and KE-37 cell lines using real-time polymerase chain reaction method. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] viability test was used to study the cell proliferation effect of galectin-9.Findings: TIM-3 mRNAs were detected in the both Jurkat and KE-37 cell lines. The expression of TIM-3 in Jurkat cell line was higher than KE-37 cell line (P < 0.001). The MTS assay revealed that galectin-9 reduced cells proliferation in a dose-dependent manner (> 1 nM) in the both cell lines (P < 0.050).Conclusion: The present investigation introduced a possible mechanism for the control of acute lymphoblastic leukemia cell proliferation through TIM-3 and demonstrated that galectin-9 can inhibit the proliferation of Jurkat and KE-37 cell lines.Background: T-cell immunoglobulin-mucin (TIM) is a cell-surface and transmembrane glycoprotein. TIM-3 plays a pivotal role in proliferation, invasion and metastasis of tumor cells. The present study was designed to evaluate the expressions of the TIM-3 and the role of the galectin-9, as TIM-3 ligand, in the regulation of cell proliferation in human acute lymphoblastic leukemia (ALL) cell lines.Methods: The expression level of TIM-3 was examined in the Jurkat and KE-37 cell lines using real-time polymerase chain reaction method. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] viability test was used to study the cell proliferation effect of galectin-9.Findings: TIM-3 mRNAs were detected in the both Jurkat and KE-37 cell lines. The expression of TIM-3 in Jurkat cell line was higher than KE-37 cell line (P < 0.001). The MTS assay revealed that galectin-9 reduced cells proliferation in a dose-dependent manner (> 1 nM) in the both cell lines (P < 0.050).Conclusion: The present investigation introduced a possible mechanism for the control of acute lymphoblastic leukemia cell proliferation through TIM-3 and demonstrated that galectin-9 can inhibit the proliferation of Jurkat and KE-37 cell lines.https://jims.mui.ac.ir/article_14698_d604dd857d8434bd10c0f2fb2c8eef03.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923Exposure with Minor Risk Factors among Blood Donors in Tehran Province, Iran: A Cohort StudyExposure with Minor Risk Factors among Blood Donors in Tehran Province, Iran: A Cohort Study1261127114699FASeyed MohammadMirrezaieAssistant Professor, Center for Health-Related Social and Behavioral Sciences Research, Shahroud University of Medical Sciences, Shahroud, IranHamid RezaSaberResearcher, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine AND Tehran Regional Educational Blood Transfusion Center, Tehran, IranFatemehNadaliAssociate Professor, Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranAliAbbasianBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine AND Tehran Regional Educational Blood Transfusion Center, Tehran, IranBashirHajibeigiBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine AND Tehran Regional Educational Blood Transfusion Center, Tehran, IranSeyed RoohallahMirrezaieLegal Medicine Organization, Semnan Province, Shahrood, IranJournal Article20150727Background: For screening and selecting healthy volunteer blood donors, it is important how to face with the minor risk factors.Methods: In a cohort study during the 2005-2011, minor risk factors for transmission of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) among blood donors in Tehran, Iran, were evaluated. Data for the study were obtained from the database of MAK-SYSTEM International Group. Odds ratio and relative risk were calculated. To calculate the incidence among repeated donors, the number of seroconverters was divided to person-years at risk.Findings: From 19,044 blood donor volunteers entered the study in 2005, 10,696 people were in the unexposed group (no risk) and 8,348 in exposed group to the minor risk factors. They were followed until 2011. The odds ratio was 2.7 for donors exposed group and 2.9 in cupping, 1.4 in dentistry, 1.5 in endoscopic plus biopsy, and 7.8 in household subgroups. Calculated relative risk for HBV was 6.9 in exposed group to the minor risk factors and 9.9 in cupping subgroup.Conclusion: The results of this study show while in the country, more accurate screening tests such as nucleic acid-based tests (NAT) or polymerase chain reaction (PCR) methods has not been used routinely for blood donors, deferral strategy applying in cases of exposure to minor risk factors, especially in the cupping exposed cases, is essential.Background: For screening and selecting healthy volunteer blood donors, it is important how to face with the minor risk factors.Methods: In a cohort study during the 2005-2011, minor risk factors for transmission of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) among blood donors in Tehran, Iran, were evaluated. Data for the study were obtained from the database of MAK-SYSTEM International Group. Odds ratio and relative risk were calculated. To calculate the incidence among repeated donors, the number of seroconverters was divided to person-years at risk.Findings: From 19,044 blood donor volunteers entered the study in 2005, 10,696 people were in the unexposed group (no risk) and 8,348 in exposed group to the minor risk factors. They were followed until 2011. The odds ratio was 2.7 for donors exposed group and 2.9 in cupping, 1.4 in dentistry, 1.5 in endoscopic plus biopsy, and 7.8 in household subgroups. Calculated relative risk for HBV was 6.9 in exposed group to the minor risk factors and 9.9 in cupping subgroup.Conclusion: The results of this study show while in the country, more accurate screening tests such as nucleic acid-based tests (NAT) or polymerase chain reaction (PCR) methods has not been used routinely for blood donors, deferral strategy applying in cases of exposure to minor risk factors, especially in the cupping exposed cases, is essential.https://jims.mui.ac.ir/article_14699_bc08ce9dbf5048faa8296452c57c64b6.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923Investigation the Effect of the Exposure Time of Docetaxel on MCF-7 Cell Line: An In-Vitro AssessmentInvestigation the Effect of the Exposure Time of Docetaxel on MCF-7 Cell Line: An In-Vitro Assessment1272128014700FAAliEbrahimifardMSc Student, Department of Medical Physics and Biomedical Engineering, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranMohammad BagherTavakoliProfessor, Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranHoseinSalehiAssistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0002-0231-4498HamidEmamiAssistant Professor, Department of Radiation and Oncology, Seyed Al-Shohada Hospital, Isfahan University of Medical Science, Isfahan, IranJournal Article20150801Background: Chemotherapy is one of the most applied methods in cancer treatment. Selection of the most effective cytostatic drug for cancer treatment is an important step in chemotherapy. Docetaxel is a drug that has especial effect when it combined with ionization radiation. The main effect of docetaxel is to block the cell cycle at the G2/M phase which has special sensitivity to the ionizing radiation. Different factors can impress the combination effect of their usage; one of them is exposure time of docetaxel with cells. In this study, we provided an experimental study about different exposure-time-induced effects of docetaxel on MCF-7 cells.Methods: We divided cells in three groups of control and 1 and 2 which were treated with different concentration of docetaxel for 5 or 24 hour, respectively. In-vitro cell viability tests were done to investigate their effect on MCF-7 cell line for 24, 48 and 72 hours after the experiment.Findings: The cytotoxicity was depended on the concentration of docetaxel. There was a significant decrease in cell viability at all concentration, especially in high concentrations for group 2 compared with group 1 which indicated time- and dose- dependency of the drug.Conclusion: Our study demonstrated that exposure time has an important effect on viability of the cells were treated with docetaxel. This phenomenon can affect treatment of cancer, especially in the field of combination therapy.Background: Chemotherapy is one of the most applied methods in cancer treatment. Selection of the most effective cytostatic drug for cancer treatment is an important step in chemotherapy. Docetaxel is a drug that has especial effect when it combined with ionization radiation. The main effect of docetaxel is to block the cell cycle at the G2/M phase which has special sensitivity to the ionizing radiation. Different factors can impress the combination effect of their usage; one of them is exposure time of docetaxel with cells. In this study, we provided an experimental study about different exposure-time-induced effects of docetaxel on MCF-7 cells.Methods: We divided cells in three groups of control and 1 and 2 which were treated with different concentration of docetaxel for 5 or 24 hour, respectively. In-vitro cell viability tests were done to investigate their effect on MCF-7 cell line for 24, 48 and 72 hours after the experiment.Findings: The cytotoxicity was depended on the concentration of docetaxel. There was a significant decrease in cell viability at all concentration, especially in high concentrations for group 2 compared with group 1 which indicated time- and dose- dependency of the drug.Conclusion: Our study demonstrated that exposure time has an important effect on viability of the cells were treated with docetaxel. This phenomenon can affect treatment of cancer, especially in the field of combination therapy.https://jims.mui.ac.ir/article_14700_f71b273d03661de9d8ded6ff7ade4b7a.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953334520150923Frequency of Complications Following Laparoscopic Cholecystectomy in Alzahra Teaching Hospital, Isfahan, Iran (2009-2011)Frequency of Complications Following Laparoscopic Cholecystectomy in Alzahra Teaching Hospital, Isfahan, Iran (2009-2011)1281128914701FABehroozKaleidariAssistant Professor, Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranHassanDehghan-BanadkukiStudent of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranMehdiKhazaeiStudent of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranShahabShahabi-ShahmiriResident, Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20140427Background: Gallstone is one of the commonly encountered diseases in the general population. Laparoscopic cholecystectomy (LC) is now accepted as the “gold standard” for the treatment of gallstone. The purpose of this study was to present our data about the type and the incidence of complications of laparoscopic cholecystectomy.Methods: A retrospective study was performed on the medical records of patients underwent laparoscopic cholecystectomy during the 2009 to 2011 in Alzahra Hospital, Isfahan, Iran. 683 laparoscopic cholecystectomies were performed during this period, of which, 314 cases were selected randomly and studied. The data were analyzed using chi-square, Fisher's exact and t tests. Findings: Among 314 studied patients, 75 (23.9%) were men and 239 (76.1%) were women. 248 cases (79.0%) with indication of biliary colic, 56 (17.8%) with acute cholecystitis, 9 (2.9%) with biliary pancreatitis and 1 (0.3%) for gallbladder polyps were operated. The complications associated to laparoscopic cholecystectomy were reported in 12 cases (3.8%). The most common complication was bile leakage in 4 patients (1.3%) occurred by stenosis after the surgery in 3 cases (1.0%). Other complications were gallstones remaining in 2 cases (0.6%), bleeding in 1 case (0.3%), thromboembolism in 1 case (0.3%) and pneumothorax in 1 case (0.3%). Conversion to open surgery was required for 4 patients (1.3%) and no deaths were reported.Conclusion: Our study suggests that laparoscopic cholecystectomy, as the procedure of choice for gallbladder stone treatment, has been associated with a high success rate and the incidence of complications was acceptably low.Background: Gallstone is one of the commonly encountered diseases in the general population. Laparoscopic cholecystectomy (LC) is now accepted as the “gold standard” for the treatment of gallstone. The purpose of this study was to present our data about the type and the incidence of complications of laparoscopic cholecystectomy.Methods: A retrospective study was performed on the medical records of patients underwent laparoscopic cholecystectomy during the 2009 to 2011 in Alzahra Hospital, Isfahan, Iran. 683 laparoscopic cholecystectomies were performed during this period, of which, 314 cases were selected randomly and studied. The data were analyzed using chi-square, Fisher's exact and t tests. Findings: Among 314 studied patients, 75 (23.9%) were men and 239 (76.1%) were women. 248 cases (79.0%) with indication of biliary colic, 56 (17.8%) with acute cholecystitis, 9 (2.9%) with biliary pancreatitis and 1 (0.3%) for gallbladder polyps were operated. The complications associated to laparoscopic cholecystectomy were reported in 12 cases (3.8%). The most common complication was bile leakage in 4 patients (1.3%) occurred by stenosis after the surgery in 3 cases (1.0%). Other complications were gallstones remaining in 2 cases (0.6%), bleeding in 1 case (0.3%), thromboembolism in 1 case (0.3%) and pneumothorax in 1 case (0.3%). Conversion to open surgery was required for 4 patients (1.3%) and no deaths were reported.Conclusion: Our study suggests that laparoscopic cholecystectomy, as the procedure of choice for gallbladder stone treatment, has been associated with a high success rate and the incidence of complications was acceptably low.https://jims.mui.ac.ir/article_14701_744124ddc0b2f3e512a0b7a7ee60b645.pdf