Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522IndexIndex15222FAJournalIndex--0000-0003-0874-1906Journal Article20171107Click to download the index of this issue.Click to download the index of this issue.https://jims.mui.ac.ir/article_15222_7d4a769562e3528950e2d1aebdfb0550.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522Classification and Similarity Analysis of Binding-Database: A Survey on Application of Multi-Class Classifiers for Deriving General Rules from Large Compound DatabasesClassification and Similarity Analysis of Binding-Database: A Survey on Application of Multi-Class Classifiers for Deriving General Rules from Large Compound Databases40040515223FAMarziehMokhtariMSc Student, Department of Biomedical Engineering AND Student Research Committee, School of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, IranAhmadMani-VarnosfaderaniAssistant Professor, Department of Chemistry, School of Basic Sciences, Tarbiat Modares University, Tehran, IranJournal Article20170118Background: In this research, we extracted and modified features of active ligands related to specific biological targets with combination of data mining and classification methods to aid medicinal chemists in their drug discovery projects. Preparing an inactive ligand is the major problem for development of multi-class classifiers. Therefore, our models were developed based on only active ligands found in Binding-database (DB) without any needs for preparing inactive molecules.Methods: Our database consisted of 160372 ligands in 45 classes of common proteins and 1497 different features (topological, chemistry, physical, etc.) were calculated for each molecule. Then, the specific features of active ligands of any target were extracted based on combination of linear discriminate analysis and Apriori algorithm.Findings: Receiver operating characteristic (ROC) was a useful operator to analysis the accuracy and sensitivity of classification models and retrieving molecules from ZINC and Binding-DB databases. Area under curve (AUC) of this diagram was evaluated for analysis of each target in Zinc and Binding-DB and their results were 0.8341 ± 0.1495 and 0.8615 ± 0.1502, respectively.Conclusion: Specific features of active ligands could be found using the methodology described in this work and with these features, we can sort each database based on corresponding target. AUC shows that the present method is useful for virtual screening in big databases without survey on inactive ligands.Background: In this research, we extracted and modified features of active ligands related to specific biological targets with combination of data mining and classification methods to aid medicinal chemists in their drug discovery projects. Preparing an inactive ligand is the major problem for development of multi-class classifiers. Therefore, our models were developed based on only active ligands found in Binding-database (DB) without any needs for preparing inactive molecules.Methods: Our database consisted of 160372 ligands in 45 classes of common proteins and 1497 different features (topological, chemistry, physical, etc.) were calculated for each molecule. Then, the specific features of active ligands of any target were extracted based on combination of linear discriminate analysis and Apriori algorithm.Findings: Receiver operating characteristic (ROC) was a useful operator to analysis the accuracy and sensitivity of classification models and retrieving molecules from ZINC and Binding-DB databases. Area under curve (AUC) of this diagram was evaluated for analysis of each target in Zinc and Binding-DB and their results were 0.8341 ± 0.1495 and 0.8615 ± 0.1502, respectively.Conclusion: Specific features of active ligands could be found using the methodology described in this work and with these features, we can sort each database based on corresponding target. AUC shows that the present method is useful for virtual screening in big databases without survey on inactive ligands.https://jims.mui.ac.ir/article_15223_853125f5451f07f8b8ed6d166f96e0c3.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522Evaluation of the Performance of Cardiopulmonary Resuscitation (CPR) Team, Shahid Chamran Hospital, Isfahan, Iran, in 2015Evaluation of the Performance of Cardiopulmonary Resuscitation (CPR) Team, Shahid Chamran Hospital, Isfahan, Iran, in 201540641115224FAMojtabaMansouriAssistant Professor, Department of Anesthesiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0002-5725-3012GholamrezaMasoumiAssociate Professor, Department of Anesthesiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0002-9182-2338Sayed AliEmamiStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranFarhadMahmoudiStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAmirrezaShokraniStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20170201Background: One of the most important issues in order to save life in patients with cardiac arrest is cardiopulmonary resuscitation (CPR). This study evaluated the performance of resuscitation team in Chamran hospital, Isfahan, Iran, in 2015.Methods: This cross-sectional study included 211 patients undergone CPR, in or out of the Chamran hospital. The CPR process data were collected from patients' files and the CPR heads filled out the checklists.Findings: The patients were 140 men (66.35%) and 71 women (33.65%). The age, sex, and location of CPR had not significant effect on the outcome of CPR (P > 0.050 for all). Use of advanced life support, the time between cardiac arrest and start of advanced life support, the time between cardiac arrest and applying first shock, use of electroshock, CPR due to basic life support (BLS), CPR due to advanced life support (ALS), and duration of CPR had significant effect in primary survey of CPR (P < 0.050 for all). Unless time between cardiac arrest and apply of first shock, there was no significant relationship between other criteria and 1-month survival after primary successful CPR (P = 0.010).Conclusion: In our study, demographic indices did not have significant effect on primary CPR outcome but need to basic and advanced life supports, applying shock to shockable rhythm and also the time between cardiac arrest and applying first shock had significant effect on primary successful CPR. Incompatibility in the percentage of CPR cases with American Heart Association (AHA) guideline notes the necessity of regular and continuous retraining.Background: One of the most important issues in order to save life in patients with cardiac arrest is cardiopulmonary resuscitation (CPR). This study evaluated the performance of resuscitation team in Chamran hospital, Isfahan, Iran, in 2015.Methods: This cross-sectional study included 211 patients undergone CPR, in or out of the Chamran hospital. The CPR process data were collected from patients' files and the CPR heads filled out the checklists.Findings: The patients were 140 men (66.35%) and 71 women (33.65%). The age, sex, and location of CPR had not significant effect on the outcome of CPR (P > 0.050 for all). Use of advanced life support, the time between cardiac arrest and start of advanced life support, the time between cardiac arrest and applying first shock, use of electroshock, CPR due to basic life support (BLS), CPR due to advanced life support (ALS), and duration of CPR had significant effect in primary survey of CPR (P < 0.050 for all). Unless time between cardiac arrest and apply of first shock, there was no significant relationship between other criteria and 1-month survival after primary successful CPR (P = 0.010).Conclusion: In our study, demographic indices did not have significant effect on primary CPR outcome but need to basic and advanced life supports, applying shock to shockable rhythm and also the time between cardiac arrest and applying first shock had significant effect on primary successful CPR. Incompatibility in the percentage of CPR cases with American Heart Association (AHA) guideline notes the necessity of regular and continuous retraining.https://jims.mui.ac.ir/article_15224_ebae5f81d1c4563ae8f8d07b9290324f.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522The Effect of Motivational Interviewing and Healthy Diet on Anthropometric Indices and Blood Pressure in Overweight and Obese School ChildrenThe Effect of Motivational Interviewing and Healthy Diet on Anthropometric Indices and Blood Pressure in Overweight and Obese School Children41242115225FAFatemehُShahriarzadehMSc Student, Department of Health Science in Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, IranRoyaKelishadiProfessor, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran0000-0001-7455-1495MaryamFatehizadehAssociate Professor, Department of Counseling, School of Education and Psychology, University of Isfahan, Isfahan, IranAkbarHassanzadehInstructor, Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, IranGholamrezaAskariAssistant Professor, Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-1194-9687Journal Article20170220Background: The prevalence of children obesity is increasing all over the world. Healthy diet is one of the interventions for obesity treatment. Behavioral therapy approaches can affect child’s food behaviors. One of these approaches is motivational interviewing.Methods: This study was a paralleled randomized clinical trial. 96 children were randomly allocated to 3 groups: first group, diet and motivational interview, second group, diet, and the third one, control. Participants were under intervention for 8 weeks. Anthropometric indecies and blood pressure were measured at baseline and at the end of the intervention. To make sure about the maintenance of intervention effects, participates were assessed 3 month later again. Adherence to diet was assessed by measuring healthy eating index.Findings: The mean body mass index (BMI) and waist circumference (WC) in first (P < 0.001) and second (P = 0.001) groups decreased after intervention, but this difference were not significant in control group. In first group, this reduction was more than the second group. The mean blood pressure had no significant difference between the 3 groups. The mean healthy eating index was higher in first group than the second group. There was an inverse relation between healthy eating index with body mass index and waist circumference (P = 0.001).Conclusion: Healthy diet can reduce mean body mass index and waist circumference in overweight and obese children. Using diet therapy with motivational interview increases this effect and also the adherence to diet.Background: The prevalence of children obesity is increasing all over the world. Healthy diet is one of the interventions for obesity treatment. Behavioral therapy approaches can affect child’s food behaviors. One of these approaches is motivational interviewing.Methods: This study was a paralleled randomized clinical trial. 96 children were randomly allocated to 3 groups: first group, diet and motivational interview, second group, diet, and the third one, control. Participants were under intervention for 8 weeks. Anthropometric indecies and blood pressure were measured at baseline and at the end of the intervention. To make sure about the maintenance of intervention effects, participates were assessed 3 month later again. Adherence to diet was assessed by measuring healthy eating index.Findings: The mean body mass index (BMI) and waist circumference (WC) in first (P < 0.001) and second (P = 0.001) groups decreased after intervention, but this difference were not significant in control group. In first group, this reduction was more than the second group. The mean blood pressure had no significant difference between the 3 groups. The mean healthy eating index was higher in first group than the second group. There was an inverse relation between healthy eating index with body mass index and waist circumference (P = 0.001).Conclusion: Healthy diet can reduce mean body mass index and waist circumference in overweight and obese children. Using diet therapy with motivational interview increases this effect and also the adherence to diet.https://jims.mui.ac.ir/article_15225_7c72900e0b6e4108fdf991b696776f6c.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522Mutations of gyrA and parC Genes in Ciprofloxacin-Resistance Isolates of Pseudomonas Aeruginosa from Burn Wounds in Yazd City, IranMutations of gyrA and parC Genes in Ciprofloxacin-Resistance Isolates of Pseudomonas Aeruginosa from Burn Wounds in Yazd City, Iran42242715226FASahar SadatEmadiDepartment of Medical Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranAhmadMosadeghInstructor, Department of Medical Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0002-3695-9681MahmoudVakiliAssociate Professor, Department of Community Medicine, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0002-0113-7100MajidEbrahimzadehDepartment of Medical Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranGolnarIzadiDepartment of Medical Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranAkramAstaniAssistant Professor, Department of Medical Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0002-6915-1320Journal Article20170114Background: Pseudomonas aeruginosa is one of the most important factors for nosocomial infections with percentage of resistance to the drug, particularly in the burn wounds. One of the mechanisms of resistance in these bacteria is chromosomal mutation in the quinolone-resistance-determining region (QRDR) of chromosome gene. The aim of this study was to evaluate gyrA and parC gene mutation in Pseudomonas aeruginosa isolates from burn wound infections resistant to ciprofloxacin.Methods: 50 clinical Pseudomonas aeruginosa isolates were identified from patients admitted to burn hospital. Minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated by E-test method and polymerase chain reaction-sequencing (PCR-sequencing) method was carried out to assess the gyrA and parC mutations in ciprofloxacin-resistant isolates.Findings: From 50 isolates, 62% were resistant to ciprofloxacin. Mutations were detected in all (100%) and 41 isolates (82%) in gyrA and parC genes, respectively. The most frequent mutations were observed in gyrA gene conversion (T83I) and parC (S87L). No mutation was found in sensitive isolates.Conclusion: Results indicate that mutations in the quinolone-resistance-determining-region are the major mechanisms for ciprofloxacin resistance in clinical isolates of Pseudomonas aeruginosa. Considering the prevalence of these genes, these mutations play a major role in the development of resistance.Background: Pseudomonas aeruginosa is one of the most important factors for nosocomial infections with percentage of resistance to the drug, particularly in the burn wounds. One of the mechanisms of resistance in these bacteria is chromosomal mutation in the quinolone-resistance-determining region (QRDR) of chromosome gene. The aim of this study was to evaluate gyrA and parC gene mutation in Pseudomonas aeruginosa isolates from burn wound infections resistant to ciprofloxacin.Methods: 50 clinical Pseudomonas aeruginosa isolates were identified from patients admitted to burn hospital. Minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated by E-test method and polymerase chain reaction-sequencing (PCR-sequencing) method was carried out to assess the gyrA and parC mutations in ciprofloxacin-resistant isolates.Findings: From 50 isolates, 62% were resistant to ciprofloxacin. Mutations were detected in all (100%) and 41 isolates (82%) in gyrA and parC genes, respectively. The most frequent mutations were observed in gyrA gene conversion (T83I) and parC (S87L). No mutation was found in sensitive isolates.Conclusion: Results indicate that mutations in the quinolone-resistance-determining-region are the major mechanisms for ciprofloxacin resistance in clinical isolates of Pseudomonas aeruginosa. Considering the prevalence of these genes, these mutations play a major role in the development of resistance.https://jims.mui.ac.ir/article_15226_d31deb89a583277bf4f76c6dcef151e3.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953542620170522The Effect of Preoperative Education on Postoperative Pain and Anxiety in Patients Undergoing Percutaneous Nephrolithotomy (PCNL)The Effect of Preoperative Education on Postoperative Pain and Anxiety in Patients Undergoing Percutaneous Nephrolithotomy (PCNL)42843315227FAMehrdadMohammadiAssistant Professor, Department of Urology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranSayed AliEmamiStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranFarhadMahmoudiStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranSaeidehBahraniStudent of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-3826-942XJournal Article20170118Background: One of the most common urologic diseases is renal and urinary tract stone. Patients experience severe pain after lithotripsy operation; one cause maybe the limited knowledge about the procedures. Due to important and considerable application of percutaneous nephrolithotomy (PCNL) procedure, we decided to evaluate the effect of preoperative education on postoperative pain and anxiety in these patients.Methods: This clinical trial study included 130 patients who underwent the percutaneous nephrolithotomy. Nephrolithiasis was confirmed via spiral computed tomography (CT) scan in them. Patients divided randomly into two groups. This study followed with two parallel groups of equal size, and the effect of preoperative education on postoperative pain and anxiety evaluated by means of visual analog scale (VAS) and Beck Anxiety Inventory (BAI), respectively. Postoperative anxiety and pain intensity were compared using independent t test.Findings: The mean anxiety level was 34.89 ± 4.84 and 22.98 ± 2.37 in control and case groups, respectively (P < 0.050). In addition, the mean pain intensity level was 74.44 ± 26.74 and 34.18 ± 8.17 in control and case groups, respectively (P < 0.050).Conclusion: In our study, postoperative stress and pain intensity after percutaneous nephrolithotomy were significantly less in experimental group. Due to less costs of preoperative educations and potential physiological and psychological effects, it is acceptable for patients to get information before surgery.Background: One of the most common urologic diseases is renal and urinary tract stone. Patients experience severe pain after lithotripsy operation; one cause maybe the limited knowledge about the procedures. Due to important and considerable application of percutaneous nephrolithotomy (PCNL) procedure, we decided to evaluate the effect of preoperative education on postoperative pain and anxiety in these patients.Methods: This clinical trial study included 130 patients who underwent the percutaneous nephrolithotomy. Nephrolithiasis was confirmed via spiral computed tomography (CT) scan in them. Patients divided randomly into two groups. This study followed with two parallel groups of equal size, and the effect of preoperative education on postoperative pain and anxiety evaluated by means of visual analog scale (VAS) and Beck Anxiety Inventory (BAI), respectively. Postoperative anxiety and pain intensity were compared using independent t test.Findings: The mean anxiety level was 34.89 ± 4.84 and 22.98 ± 2.37 in control and case groups, respectively (P < 0.050). In addition, the mean pain intensity level was 74.44 ± 26.74 and 34.18 ± 8.17 in control and case groups, respectively (P < 0.050).Conclusion: In our study, postoperative stress and pain intensity after percutaneous nephrolithotomy were significantly less in experimental group. Due to less costs of preoperative educations and potential physiological and psychological effects, it is acceptable for patients to get information before surgery.https://jims.mui.ac.ir/article_15227_16db016ed3a5b8d7c596928306161ada.pdf