Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953856720200420IndexIndex1603810.22122/jims.v38i567.13387FAJournalIndex0000-0003-0874-1906Journal Article20200828Click to download the index of this issue.Click to download the index of this issue.https://jims.mui.ac.ir/article_16038_96fb9b48825b741083d35b0137af1be0.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953856720200420Evaluation of Cytotoxic Effects of the Combination of Metformin with Docetaxel and 5-Fluorouracil on the Gastric Cancer CellsEvaluation of Cytotoxic Effects of the Combination of Metformin with Docetaxel and 5-Fluorouracil on the Gastric Cancer Cells1391461603910.22122/jims.v38i567.12384FAMaryamFatehi-AghdamMSc Student, Department of Anatomical Sciences and Pathology, School of Medicine and Paramedicine, Ardabil University of Medical Sciences, Ardabil, Iran0000-0002-6517-9553NowruzNajafzadehAssociate Professor, Research Laboratory for Embryology and Stem Cells AND Department of Anatomical Sciences and Pathology, School of Medicine and Paramedicine, Ardabil University of Medical Sciences, Ardabil, Iran0000-0001-8870-1255Mohammad GhasemGolmohammadiAssociate Professor, Research Laboratory for Embryology and Stem Cells AND Department of Anatomical Sciences and Pathology, School of Medicine and Paramedicine, Ardabil University of Medical Sciences, Ardabil, Iran0000-0002-8454-6558Journal Article20191016Background: In the present study, the cytotoxic effects of the combination of metformin with docetaxel and 5- fluorouracil were studied on AGS gastric cancer cell line.Methods: In this experimental study, the cells were exposed to different concentrations of metformin (1-80 mM), docetaxel (0.6-22.5 ng/ml), 5-fluorouracil (0.045-12 μg/ml), and combination of them. The cell proliferation was evaluated using MTT assay, and the cell apoptosis was evaluated by acridine orange/ethidium bromide assay.Findings: Metformin, docetaxel, and 5-fluorouracil inhibited cell viability of AGS gastric cancer cells in a concentration- and time-dependent manner. The half-maximal inhibitory concentration (IC50) values were decreased after treatment with metformin/docetaxel and metformin/5-fluorouracil (P < 0.050). Moreover, the combination treatments significantly increased apoptosis of gastric cancer cells (P < 0.050).Conclusion: Our results showed that the combination of metformin with docetaxel and 5-fluorouracil could be effective in the treatment of patients with gastric cancer.Background: In the present study, the cytotoxic effects of the combination of metformin with docetaxel and 5- fluorouracil were studied on AGS gastric cancer cell line.Methods: In this experimental study, the cells were exposed to different concentrations of metformin (1-80 mM), docetaxel (0.6-22.5 ng/ml), 5-fluorouracil (0.045-12 μg/ml), and combination of them. The cell proliferation was evaluated using MTT assay, and the cell apoptosis was evaluated by acridine orange/ethidium bromide assay.Findings: Metformin, docetaxel, and 5-fluorouracil inhibited cell viability of AGS gastric cancer cells in a concentration- and time-dependent manner. The half-maximal inhibitory concentration (IC50) values were decreased after treatment with metformin/docetaxel and metformin/5-fluorouracil (P < 0.050). Moreover, the combination treatments significantly increased apoptosis of gastric cancer cells (P < 0.050).Conclusion: Our results showed that the combination of metformin with docetaxel and 5-fluorouracil could be effective in the treatment of patients with gastric cancer.https://jims.mui.ac.ir/article_16039_009d69d2d1b986815e7b825382d73af5.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953856720200420The Comparison of the Effect of Handmade Del Nido and St. Thomas Cardioplegia Solutions on Quantitative Levels of Troponin I and Creatine Kinase-Myocardial Band (CPK-MB) in Repair of Pediatric Ventricular Septal DefectThe Comparison of the Effect of Handmade Del Nido and St. Thomas Cardioplegia Solutions on Quantitative Levels of Troponin I and Creatine Kinase-Myocardial Band (CPK-MB) in Repair of Pediatric Ventricular Septal Defect1471531604010.22122/jims.v38i567.12155FAHamidBigdelianAssistant Professor, Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0001-9222-9184MortezaSadeghiMSc Student of Perfusion Science, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-2369-1564Journal Article20190815Background: The most common cardioplegic solutions in pediatric cardiac surgery are Del Nido and St. Thomas. The aim of this study was to compare of the effect of these two solutions on cardiac protection, and prevention of myocardial injury during the aortic clamp time.Methods: This randomize clinical trial study was accomplished on 60 children under 12 years who were candidates for large ventral septal defect (VSD) repair surgery at Shahid Chamran hospital in Isfahan, Iran. One of handmade St. Thomas or Del Nido cardioplegic solutions was used randomly for electromechanical cardiac arrest in each children. Quantitative levels of cardiac troponin I and creatine kinase-myocardial band (CPK-MB) was measured and recorded before and 6 and 12 hours after the surgery.Findings: There was no significant difference between the two markers of troponin I and CPK-MB in both groups (P > 0.050). There was no difference regarding the duration of cardiopulmonary bypass (P = 0.120) and cardiac rhythm return time (P = 0.200) after removal of aortic clamp in both groups. The time of aortic clamp was significantly shorter in Del Nido group (P = 0.009).Conclusion: Due to the lack of statistically significant difference in quantitative levels of troponin I and CPK-MB between the two groups, it seems that efficiency of both solutions are similar.Background: The most common cardioplegic solutions in pediatric cardiac surgery are Del Nido and St. Thomas. The aim of this study was to compare of the effect of these two solutions on cardiac protection, and prevention of myocardial injury during the aortic clamp time.Methods: This randomize clinical trial study was accomplished on 60 children under 12 years who were candidates for large ventral septal defect (VSD) repair surgery at Shahid Chamran hospital in Isfahan, Iran. One of handmade St. Thomas or Del Nido cardioplegic solutions was used randomly for electromechanical cardiac arrest in each children. Quantitative levels of cardiac troponin I and creatine kinase-myocardial band (CPK-MB) was measured and recorded before and 6 and 12 hours after the surgery.Findings: There was no significant difference between the two markers of troponin I and CPK-MB in both groups (P > 0.050). There was no difference regarding the duration of cardiopulmonary bypass (P = 0.120) and cardiac rhythm return time (P = 0.200) after removal of aortic clamp in both groups. The time of aortic clamp was significantly shorter in Del Nido group (P = 0.009).Conclusion: Due to the lack of statistically significant difference in quantitative levels of troponin I and CPK-MB between the two groups, it seems that efficiency of both solutions are similar.https://jims.mui.ac.ir/article_16040_af12e5e50bc88cf41244cf4ced8988c7.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953856720200420Recent Findings of Coronavirus: The Pathogenesis and TreatmentRecent Findings of Coronavirus: The Pathogenesis and Treatment1541641604110.22122/jims.v38i567.12889FARezaBastanAssistant Professor, Department of Human Vaccines, Razi Serum and Vaccine Research Institute, Karaj, Iran0000-0001-6025-2822NedaKasiriDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0001-6025-2822NahidEskandariAssociate Professor, Department of Immunology, School of Medicine AND Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-1910-9359Journal Article20200330Coronavirus disease-2019 (COVID-19) is a type of acute viral pneumonia, which began with a sudden outbreak in Wuhan, China. Although closely related to severe acute respiratory syndrome, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), the disease excessive transmission rate distinguishes it from other viral pneumonia diseases. The pulmonary complications and infections caused by this virus, as well as the lack of specific therapeutic have caused one of the most complicated epidemics of the last century. In this review article, we attempted to discuss and describe the latest findings of immunopathogenesis, as well new and applicable treatments for coronavirus disease.Coronavirus disease-2019 (COVID-19) is a type of acute viral pneumonia, which began with a sudden outbreak in Wuhan, China. Although closely related to severe acute respiratory syndrome, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), the disease excessive transmission rate distinguishes it from other viral pneumonia diseases. The pulmonary complications and infections caused by this virus, as well as the lack of specific therapeutic have caused one of the most complicated epidemics of the last century. In this review article, we attempted to discuss and describe the latest findings of immunopathogenesis, as well new and applicable treatments for coronavirus disease.https://jims.mui.ac.ir/article_16041_3e49d4ec10aad7c623659c37e011665a.pdf