Isfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953860120210120Ocular Perfusion Pressure in Patients with Acute Ischemic Central Retinal Vein Obstruction in Comparing with Control GroupOcular Perfusion Pressure in Patients with Acute Ischemic Central Retinal Vein Obstruction in Comparing with Control Group8698741616610.22122/jims.v38i601.13031FAAliSalehiAssociate Professor, Department of Ophthalmology, School of Medicine AND Eye Research Center, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-3269-9656AfsanehNaderi-BeniAssistant Professor, Department of Ophthalmology, School of Medicine AND Eye Research Center, Isfahan University of Medical Sciences, Isfahan, Iran0000-0003-2958-7805MohammadArastoueiResident, Department of Ophthalmology, School of Medicine AND Eye Research Center, Isfahan University of Medical Sciences, Isfahan, Iran0000-0001-8497-7713Journal Article20200504Background: Ocular perfusion pressure (OPP) can be used as a predictive factor for the severity and prognosis of eye diseases. The aim of this study was to evaluate the rate of ocular perfusion pressure in ischemic retinal vein ischemia.Methods: This cross-sectional study was performed in two groups of 44 patients (patient and control) in Feyz hospital, Isfahan, Iran. The diagnosis of acute central retinal vein occlusion (CRVO) was made on the basis of clinical criteria. Effective risk factors and mean arterial pressure (MAP), OPP, intraocular pressure (IOP) were measured. The results were analyzed using SPPS software.Findings: OPP was 59.38 ± 4.35 in patients with CRVO and 53.49 ± 6.45 in the control group, and a significant difference was observed (P = 0.004). Intraocular pressure was 15.15 ± 2.61 and 13.77 ± 2.12 in the CRVO and control groups, respectively, with a significant difference (P = 0.008).Conclusion: OPP and also the IOP were significantly higher in the CRVO group. Increasing of OPP can be due to a relative increase in blood pressure (BP) or a relative increase in IOP, each of which alone or together can lead to vascular events. Ocular perfusion pressure is used as a risk factor of CRVO.Background: Ocular perfusion pressure (OPP) can be used as a predictive factor for the severity and prognosis of eye diseases. The aim of this study was to evaluate the rate of ocular perfusion pressure in ischemic retinal vein ischemia.Methods: This cross-sectional study was performed in two groups of 44 patients (patient and control) in Feyz hospital, Isfahan, Iran. The diagnosis of acute central retinal vein occlusion (CRVO) was made on the basis of clinical criteria. Effective risk factors and mean arterial pressure (MAP), OPP, intraocular pressure (IOP) were measured. The results were analyzed using SPPS software.Findings: OPP was 59.38 ± 4.35 in patients with CRVO and 53.49 ± 6.45 in the control group, and a significant difference was observed (P = 0.004). Intraocular pressure was 15.15 ± 2.61 and 13.77 ± 2.12 in the CRVO and control groups, respectively, with a significant difference (P = 0.008).Conclusion: OPP and also the IOP were significantly higher in the CRVO group. Increasing of OPP can be due to a relative increase in blood pressure (BP) or a relative increase in IOP, each of which alone or together can lead to vascular events. Ocular perfusion pressure is used as a risk factor of CRVO.https://jims.mui.ac.ir/article_16166_d801eb0fd6c4a9a6d5f7e5c76c6a60fc.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953860120210120Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform GlioblastomaInvestigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma8758811616710.22122/jims.v38i601.13007FAFahimehZamani-RaraniPhD Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0002-6367-8367ZeinolabedinShrifian-DastjerdiAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranAliValianiAssociate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranMohammadZamani-RaraniAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran0000-0003-2841-7927EliasKargar-AbargoueiAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran0000-0002-8520-6344EbrahimEftekharAssociate Professor, Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Science, Bandar Abbas, Iran0000-0002-4519-0595MajidPourentezariAssistant Professor, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0001-7549-4672JavadMohajer-AnsariAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran0000-0002-5090-592XJournal Article20200430Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied the role of agonist (N6-cyclopentyladenosine or CPA) and antagonist (8-cyclopentyl-1,3-dipropylxanthine or DPCPX) of this receptor on cell apoptosis and also expression of P53 genes and caspases 7, 8, and 9.Methods: In this study, MTT assay was used to investigate the rate of cellular proliferation, and flowcytometry method with annexin and Pi was also used to investigate early and late cell apoptosis. To evaluate the internal and external apoptotic pathways expression of P53 genes and caspases 7, 8, and 9, real-time reverse transcription polymerase chain reaction (real-time RT PCR) was used.Findings: The treatment of U87Mg cells with DPCPX increased the expression of P53 gene. Expression of caspase 7 as an executive caspase and caspase 9 as a caspase of the mitochondrial pathway of apoptosis increased, but no expression change was observed in the caspase 8 gene.Conclusion: The results of MTT and flowcytometry showed that DPCPX, in addition to suppressing cell proliferation, stimulated apoptosis in U87Mg cells. Inhibition of adenosine A1 receptors by stimulating the expression of genes involved in apoptotic pathways, especially mitochondrial pathway genes, suppressed cell proliferation and induced apoptosis in U87Mg cells.Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied the role of agonist (N6-cyclopentyladenosine or CPA) and antagonist (8-cyclopentyl-1,3-dipropylxanthine or DPCPX) of this receptor on cell apoptosis and also expression of P53 genes and caspases 7, 8, and 9.Methods: In this study, MTT assay was used to investigate the rate of cellular proliferation, and flowcytometry method with annexin and Pi was also used to investigate early and late cell apoptosis. To evaluate the internal and external apoptotic pathways expression of P53 genes and caspases 7, 8, and 9, real-time reverse transcription polymerase chain reaction (real-time RT PCR) was used.Findings: The treatment of U87Mg cells with DPCPX increased the expression of P53 gene. Expression of caspase 7 as an executive caspase and caspase 9 as a caspase of the mitochondrial pathway of apoptosis increased, but no expression change was observed in the caspase 8 gene.Conclusion: The results of MTT and flowcytometry showed that DPCPX, in addition to suppressing cell proliferation, stimulated apoptosis in U87Mg cells. Inhibition of adenosine A1 receptors by stimulating the expression of genes involved in apoptotic pathways, especially mitochondrial pathway genes, suppressed cell proliferation and induced apoptosis in U87Mg cells.https://jims.mui.ac.ir/article_16167_c8b4d673d23385e09789e7db8e3f431a.pdfIsfahan University of Medical SciencesJournal of Isfahan Medical School1027-75953860120210120Investigation the Radiation Effects of Natural-Enriched Uanium Hexafluoride (UF6) Gas Leakage by RASCAL Software SimulationInvestigation the Radiation Effects of Natural-Enriched Uanium Hexafluoride (UF6) Gas Leakage by RASCAL Software Simulation8828851616810.22122/jims.v38i601.12674FAMasoudSaebMSc Student, Department of Health and Environment, Najafabad Branch, Islamic Azad University, Najafabad, Iran0000-0002-3331-7501DaryoushShahbazi-GahroueiProfessor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran0000-0002-8538-1898Journal Article20200127Background: Radioactive release materials in nuclear facilities causes environmental pollution, and absorption of radiations by respiration by people and staff in the area of the accident is a vital problem. The aim of this study was to stimulate the event using Radiological assessment system for consequence analysis (RASCAL) software and compare the results with those of Gaussian experimental model.Methods: In this study, the accident included Uanium hexafluoride (UF6) gas leakage in the environment from the storage areas of the Sooreh Company, Isfahan, Iran. The geographical distribution of radioactive material and the received amount of staff and people around the site were estimated at a radius of ten kilometers.Findings: Uranium hexafluoride gas leakage event had the highest amount of radioactivity release that caused absorbed dose to people and staff in the company fence line.Conclusion: Findings showed that Uranium hexafluoride would not threaten any deterministic effects on staff and people. Also, it is recommended to use software codes to calculate event and radiation risk.Background: Radioactive release materials in nuclear facilities causes environmental pollution, and absorption of radiations by respiration by people and staff in the area of the accident is a vital problem. The aim of this study was to stimulate the event using Radiological assessment system for consequence analysis (RASCAL) software and compare the results with those of Gaussian experimental model.Methods: In this study, the accident included Uanium hexafluoride (UF6) gas leakage in the environment from the storage areas of the Sooreh Company, Isfahan, Iran. The geographical distribution of radioactive material and the received amount of staff and people around the site were estimated at a radius of ten kilometers.Findings: Uranium hexafluoride gas leakage event had the highest amount of radioactivity release that caused absorbed dose to people and staff in the company fence line.Conclusion: Findings showed that Uranium hexafluoride would not threaten any deterministic effects on staff and people. Also, it is recommended to use software codes to calculate event and radiation risk.https://jims.mui.ac.ir/article_16168_8d566a338d7758ba07b0fade172590cc.pdf