Document Type : Review Article
Authors
1
Assistant Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
2
Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
10.48305/jims.2022.41138.1194
Abstract
Background : The emergence of Extended-Spectrum Beta-Lactamase- and Carbapenemase- Producing Enterobacteriaceae is a threat to global health. Fast and correct detection of these strains plays a key role in controlling nosocomial infections and correct treatment. In present study, the detection methods of carbapenemase and broad-spectrum β-lactam-producing Enterobacteriaceae have been investigated, focusing on the summary of culture-based techniques and molecular methods.
Methods: The present study is a narrative review. The articles published between 2000 and 2022 were searched in international authoritative databases Scopus, PubMed, Scholar, Google, Web of Science, Science direct.
Findings: To identify Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, conventional culture methods, biochemical methods, mass spectrometry, molecular methods, next generation sequencing, microarrays, whole genome sequencing, hybridization-based system, starch iodine assay, immunochromatography, colorimetry, mass spectrometry, electrochemical measurement, and flow cytometry are used.
Conclusion: To detect of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight), next-generation sequencing and whole genome sequencing are among the new and selected techniques.
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