اثر محافظتی سیر بر سمیت ناشی از استرس اکسیداتیو آمینوگلیکوزیدها

نصری, حمید; رفیعیان کوپایی, محمود

اثر محافظتی سیر بر سمیت ناشی از استرس اکسیداتیو آمینوگلیکوزیدها

نوع مقاله : مقاله های پژوهشی

نویسندگان

¹ استاد، گروه نفروپاتولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

² استاد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

چکیده

مقدمه: نفروتوکسیسیتی ناشی از مصرف آمینوگلیکوزیدها به تولید گونه‌های آزاد اکسیژنی (ROS یا Reactive oxygen species) در کلیه نسبت داده شده است. سیر دارای خواص آنتی اکسیدانی است و هدف از این مطالعه، بررسی اثر سیر بر بهبود نفروتوکسیسیتی ناشی از جنتامایسین بود.روش‌ها: در یک تحقیق مداخله‌ای، 50 رت نر نژاد ویستار در 5 گروه با تعداد مساوی بدین شرح تقسیم شدند: گروه I بدون دریافت دارو، گروه II دریافت کننده‌ی جنتامایسین (mg/kg 100 به مدت 10 روز)، گروه III گروه عصاره‌ی سیر (mg/kg 20 از طریق تزریق داخل صفاقی به مدت 10 روز)، گروه IV گروهی که ابتدا جنتامایسین و سپس عصاره‌ی سیر (mg/kg 20 به مدت 10 روز) دریافت کرد و گروه V گروه جنتامایسین و عصاره‌ی سیر به طور همزمان (10 روز). نیتروژن اوره‌ی خون (BUN یا Blood urea nitrogen) و کراتینین (Cr یا Creatinine) سرم اندازه‌گیری و کلیه‌ها از نظر معیارهای مورفولوژیکی درگیر کننده‌ی سلول توبولی بررسی و با یکدیگر مقایسه شدند.یافته‌ها: مصرف سیر پس از مصرف جنتامایسین سطوح BUN و Cr را به طور قوی کاهش داد (050/0 > P). مصرف 10 روزه‌ی سیر پس از درمان با جنتامایسین، درجه‌ی آسیب پاتولوژی را نیز به طور معنی‌داری برگشت داد.نتیجه‌گیری: سیر یک داروی نفروپروتکتیو است که شاید بتوان به منظور کاهش آسیب توبولی ایجاد شده توسط جنتامایسین از آن استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Preventive and Curative Activity of Garlic Extract on Gentamicin-Induced Oxidative Stress

نویسندگان [English]

Hamid Nasri ¹
Mahmoud Rafieian-Kopaei ²

¹ Professor, Department of Nephrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

² Professor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

چکیده [English]

Background: Nephrotoxicity is a serious side effect of using gentamicin. Garlic is an important component in the complementary medicine. The aim of this study was to find out whether garlic has ameliorative effects on gentamicin-induced nephrotoxicity.Methods: 50 male Wistar rats were divided into 5 groups of 10; I: They kept in the same condition as others without receiving any drug for 10 days and then sacrificed. II: Rats in this group were injected intraperitoneally (i.p.) with 100 mg/kg of gentamicin for 10 consecutive days and then sacrificed. Group III: Rats in this group received garlic juice 20 mg/kg intraperitoneally for 10 days and then sacrificed. IV: Rats in this group received gentamicin for 10 days, then received 20 mg/kg garlic intraperitoneally for the next 10 days and then sacrificed on day 20th. V: Rats in this group received a combination of intraperitoneal gentamicin and garlic 20 mg/kg for 10 days and then sacrificed. Serum blood urea nitrogen (BUN) and creatinine (Cr) were measured and the kidneys were processed for histopathological examinations. All specimens were examined for morphologic parameters involving tubular cells.Findings: The post administration of garlic after gentamicin treatment potentially attenuated the serum levels of BUN and Cr. The pathology damage scores indicated that post administration of garlic after 10 days of gentamicin treatment attenuated the damage score significantly.Conclusion: We concluded that garlic is a nephroprotective drug to attenuate tubular injury by gentamicin.

کلیدواژه‌ها [English]

Garlic
Gentamicine
Nephrotoxicity

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