Evaluation of Prevalence of the New Candida Species (C. Orthopsilosis and C. Metapsilosis) among C. Parapsilosis Group Isolated from Patients by PCR-RFLP of SADH Gene in Iran

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran.

2 Associate Professor, Department of Medical Mycology and Parasitology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.

3 Associate Professor, Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran.

4 Assistant Professor, Department of Medical Mycology, School of Basic Sciences, Imam Hossein University, Tehran, Iran.

5 Professor, Department of Medical Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

6 National Institute of Health Research, Tehran University of Medical Sciences, Tehran Iran.

7 Professor, Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Abstract

Background: Candida orthopsilosis and C. metapsilosis are recently introduced species that phenotipically indistinguishable from C. parapsilosis. In the present study, we evaluated the incidence and distribution of C. orthopsilosis and C. metapsilosis among 111 C. parapsilosis, isolated from different body locations by molecular methods.Methods: Yeast genomic DNAs were extracted from 935 isolates using Whatman FTA filter matrix technology from fresh cultures. First, ITS region of yeasts, were amplified by ITS1 and ITS4 primers, then Candida parapsilosis group were identified with digestion by MspI. SADH gene was amplified and three species of Candida including C. parapsilosis, C. orthopsilosis and C. metapsilosis were differentiated with digestion by NlaIII enzyme.Finding: Totally 111 isolates were identified as Candida parapsilosis among them, 18 isolates (16.2%) were identified as C. orthopsilosis and 93 isolates (83.7%) as C. parapsilosis. No isolates were identified as C. metapsilosis. The most isolates of C. orthopsilosis, were obtained from nail.Conclusion: C. ortopsilosis is not a rare species in Iran and it seems that a significant amounts of Candida strains which are considered as C. parapsilosis might be C. ortopsilosis.

Keywords

References

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Journal of Isfahan Medical School
Volume 29, Issue 134 - Serial Number 134
March and April 2011
Pages 376-385

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History

  • Receive Date: 01 April 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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