بررسی سمیت عصاره‌ی هیدروالکلی هندوانه‌ی کوهی Momordica Charantia بر کبد موش‌های سوری نر

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 استاد، گروه داخلی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 استادیار، گروه داخلی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

3 استاد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

چکیده

مقدمه: هندوانه‌ی کوهی، به علت خاصیت هیپوگلایسمیک شهرت دارد و به صورت گسترده در جامعه جهت درمان دیابت استفاده می‌شود. در این تحقیق، علاوه بر بررسی ظرفیت آنتی‌اکسیدانی گیاه و اثر آن بر ظرفیت آنتی‌اکسیدانی خون، احتمال هپاتوتوکسیسیتی این گیاه با بررسی شیمیایی و هیستولوژیک مورد ارزیابی قرار گرفت.روش‌ها: در این مطالعه، 70 موش سوری نر با وزن 30-25 گرم وارد مطالعه شدند و به طور تصادفی در 7 گروه قرار گرفتند. موش‌ها به مدت 7 روز در شرایط آزمایشگاهی نگهداری و سپس در روز هشتم دوزهای مختلف عصاره‌ی هندوانه‌ی کوهی از طریق تزریق داخل صفاقی دریافت کردند. گروه‌‌های درمان تک دوز شامل 100، 500، 1000، 2000 و 4000 میلی‌گرم بر کیلوگرم و گروهی که یک هفته تحت درمان با دوز 500 میلی‌گرم بر کیلوگرم به صورت روزانه قرار گرفت. سپس به مدت 72 ساعت موش‌ها تحت نظر بودند و بعد از آن کشته شدند. کبد موش‌ها جهت بررسی هیستولوژی برداشته و تغییرات هیستوپاتولوژیک کبد بررسی شد. سرم موش‌ها نیز از نظر آنزیم‌های کبدی (ALP یا Alkaline phosphatase، SGPT یا Serum glutamic-pyruvic transaminase و SGOT یا Serum glutamic oxaloacetic transaminase) بررسی شد. ظرفیت آنتی‌اکسیدانی خون و گیاه نیز محاسبه شد.یافته‌ها: در کلیه‌ی گروه‌هایی که دارو را به صورت تک دوز دریافت کرده بودند، تغییرات آماری معنی‌داری در میزان آنزیم‌های کبدی SGOT، SGPT و ALP و همچنین در مطالعات بافت‌شناسی مشاهده نشد (05/0 < P). مقادیر آنزیم‌های SGOT، SGPT و ALP به ترتیب 0/76 ± 8/180، 0/54 ± 6/80 و 0/88 ± 7/204 بود. ظرفیت آنتی‌اکسیدانی هندوانه‌ی کوهی 68 درصد و ظرفیت آنتی‌اکسیدانی خون 564 میکرومول بر لیتر به دست آمد.نتیجه‌گیری: هندوانه‌ی کوهی تا دوز 4000 میلی‌گرم بر کیلوگرم به صورت تک دوز برای کبد آسیب و سمیتی ایجاد نمی‌کند، اما انجام مطالعات بیشتر با دوز‌های مختلف دارو پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluating the Liver Toxicity of Hydroalcoholic Extract of Momordica Charantia in Male Balb/C Mice

نویسندگان [English]

  • Hamid Nasri 1
  • Saeed Mardani 2
  • Mahmoud Rafieian-Kopaei 3
1 Professor, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Internal Medicine, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Professor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده [English]

Background: Momordica charantia (bitter melon) is known for its hypoglycemic effect and widely used for the treatment of diabetes. This study other than evaluating plant antioxidant and its effect on blood antioxidant capacity, examined the effects and safety of bitter melon fruit in laboratory mice.Methods: 70 male mice (2-3 weeks old, body weight 25-30 g) were randomly divided into 7 groups. The mice were acclimatized to laboratory conditions for 7 days and at day 8, they were dosed intraperitoneally (single dose groups: 0, 100, 500, 1000, 2000, 4000 mg/kg and the group which was treated for 7 days: 500 mg/kg/day). Mice were then observed for 72 hours before they were scarified, immediately livers were taken for histology. Serum samples were assayed for liver functions [alkaline phosphatase (ALT), serum glutamic-pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT)]. Blood and bitter melon antioxidant activity was measured.Findings: All single dose groups showed normal behavior after the dosing and no statistical changes were observed in all liver parameters including for SGOT, SGPT or ALP (P > 0.05). Lab data were shown as follow: ALP = 204.7 ± 88.0, SGOT = 180.8 ± 76.0, SGPT = 80.6 ± 54.0. Histological examinations revealed normal organ structures. Antioxidant activity of bitter melon was 68% and blood antioxidant activity was 564 µmol/ml.Conclusion: Doses up to 4000 mg/kg did not have any effects on the mice liver functions nor its histology. We suggest more studies with different doses.

کلیدواژه‌ها [English]

  • Momordica charantia
  • Liver
  • Alkaline phosphatase (ALT)
  • Serum glutamic-pyruvic transaminase (SGPT)
  • Serum glutamic oxaloacetic transaminase (SGOT)

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مجله دانشکده پزشکی اصفهان
دوره 34، شماره 376 - شماره پیاپی 376
فروردین و اردیبهشت 1395
صفحه 277-284

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  • تاریخ دریافت: 18 شهریور 1394
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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